A novel reassortant mammalian orthoreovirus with a divergent S1 genome segment identified in a traveler with diarrhea

Mikuletič, Tina; Steyer, Andrej; Kotar, Tadeja; Zorec, Tomaž Mark; Poljak, Mario

doi:10.1016/j.meegid.2019.06.002

Cited by 8 publications

(3 citation statements)

References 19 publications

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“…These results suggested that there is little antigenic variation among the σ2 proteins of these viruses. While the M3 segments of MRV-85 and MRV-96 shared the highest nucleotide and amino acid identities with those of the porcine strain (BM-100) (90.1–90.3 and 95.5–95.7 %, respectively) [2], the pairwise nucleotide and amino acid sequence identities of the M3 segment of MRV-117 with that of SI-MRV07 identified in patients with diarrhea who traveled to Southeast Asia was 89.8 and 95.7 %, respectively [39]. The S3 segment of MRV-96 shared the highest identity with the HB-B strain isolated from minks in China [21], whereas the S3 segments of the MRV-85 and MRV-117 strains shared the highest sequence identities with the T1/Human/Wash.D.C./clone62/1957 and T3/Bovine/Maryland/clone18/1961 strains [40].…”

Section: Resultsmentioning

confidence: 99%

“…Contrarily, the M3 segment of MRV-117 most likely originated from human strains, such as SI-MRV07 and CMR-HP55, but there is no information about human MRV strains in Zambia. Interestingly, these strains were recently detected in patients with gastroenteritis in South Asia and Africa [27,39], thus suggesting that MRV causing gastroenteritis in humans might present in Zambia. Understanding the consequences of viral reassortment is important to prepare for newly emerging high pathogenic viruses, and further surveillance of MRVs circulating in human and other animals in Zambia is required to enhance veterinary and public health.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

Harima

Sasaki

Kajihara

et al. 2020

Journal of General Virology

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Mammalian orthoreovirus (MRV) has been identified in humans, livestock and wild animals; this wide host range allows individual MRV to transmit into multiple species. Although several interspecies transmission and genetic reassortment events of MRVs among humans, livestock and wildlife have been reported, the genetic diversity and geographic distribution of MRVs in Africa are poorly understood. In this study, we report the first isolation and characterization of MRVs circulating in a pig population in Zambia. In our screening, MRV genomes were detected in 19.7 % (29/147) of faecal samples collected from pigs by reverse transcription PCR. Three infectious MRV strains (MRV-85, MRV-96 and MRV-117) were successfully isolated, and their complete genomes were sequenced. Recombination analyses based on the complete genome sequences of the isolated MRVs demonstrated that MRV-96 shared the S3 segment with a different MRV isolated from bats, and that the L1 and M3 segments of MRV-117 originated from bat and human MRVs, respectively. Our results suggest that the isolated MRVs emerged through genetic reassortment events with interspecies transmission. Given the lack of information regarding MRVs in Africa, further surveillance of MRVs circulating among humans, domestic animals and wildlife is required to assess potential risk for humans and animals.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

Harima

Sasaki

Kajihara

et al. 2020

Journal of General Virology

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“…Based on antigenicity, MRVs are subdivided into four groups: type 1 Lang, type 2 Jones, type 3 Dearing, and type 4 Ndelle ( 3 ). MRVs are commonly detected in humans, mammals, and aquatic environments ( 3 – 8 ). Although MRV infections in humans have previously been considered mild or asymptomatic ( 9 ), recent studies have indicated that they may cause respiratory or gastroenteritis problems in humans, especially in children ( 10 , 11 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization and pathogenicity in a mouse model of newly isolated bat-originated mammalian orthoreovirus in South Korea

Do,

Yeom,

Moon

et al. 2024

Microbiol Spectr

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Mammalian orthoreoviruses (MRVs) infect a wide range of hosts, including humans, livestock, and wildlife. In the present study, we isolated a novel Mammalian orthoreovirus from the intestine of a microbat ( Myotis aurascens ) and investigated its biological and pathological characteristics. Phylogenetic analysis indicated that the new isolate was serotype 2, sharing the segments with those from different hosts. Our results showed that it can infect a wide range of cell lines from different mammalian species, including human, swine, and non-human primate cell lines. Additionally, media containing trypsin, yeast extract, and tryptose phosphate broth promoted virus propagation in primate cell lines and most human cell lines, but not in A549 and porcine cell lines. Mice infected with this strain via the intranasal route, but not via the oral route, exhibited weight loss and respiratory distress. The virus is distributed in a broad range of organs and causes lung damage. In vitro and in vivo experiments also suggested that the new virus could be a neurotropic infectious strain that can infect a neuroblastoma cell line and replicate in the brains of infected mice. Additionally, it caused a delayed immune response, as indicated by the high expression levels of cytokines and chemokines only at 14 days post-infection (dpi). These data provide an important understanding of the genetics and pathogenicity of mammalian orthoreoviruses in bats at risk of spillover infections. IMPORTANCE Mammalian orthoreoviruses (MRVs) have a broad range of hosts and can cause serious respiratory and gastroenteritis diseases in humans and livestock. Some strains infect the central nervous system, causing severe encephalitis. In this study, we identified BatMRV2/SNU1/Korea/2021, a reassortment of MRV serotype 2, isolated from bats with broad tissue tropism, including the neurological system. In addition, it has been shown to cause respiratory syndrome in mouse models. The given data will provide more evidence of the risk of mammalian orthoreovirus transmission from wildlife to various animal species and the sources of spillover infections.

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Molecular characterization of an emerging reassortant mammalian orthoreovirus in China

Shi

et al. 2020

Arch Virol

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A novel reassortant mammalian orthoreovirus with a divergent S1 genome segment identified in a traveler with diarrhea

Cited by 8 publications

References 19 publications

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

Characterization of mammalian orthoreoviruses isolated from faeces of pigs in Zambia

Genetic characterization and pathogenicity in a mouse model of newly isolated bat-originated mammalian orthoreovirus in South Korea

Molecular characterization of an emerging reassortant mammalian orthoreovirus in China

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