A novel paper based immunoassay of breast cancer specific carbohydrate (CA 15.3) using silver nanoparticles-reduced graphene oxide nano-ink technology: A new platform to construction of microfluidic paper-based analytical devices (μPADs) towards biomedical analysis

Hassanpour, Soodabeh; Hasanzadeh, Mohammad; Saadati, Arezoo; Shadjou, Nasrin; Soleymani, Jafar; Jouyban, Abolghasem

doi:10.1016/j.microc.2019.01.018

Cited by 59 publications

(26 citation statements)

References 66 publications

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“…Newly, designing conductive models on paper has used as affordable and easy technique for sketching paper-based sensors [ 18 ]. So this technique helps to have low-cost and rapid screening to designing the customized electrodes.…”

Section: Introductionmentioning

confidence: 99%

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

Farshchi

Saadati

Hasanzadeh

2020

Heliyon

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Prostate cancer is the most significant reason for deaths in men, outside of lung cancer. The clinical examination of cancer proteins or biomarkers is extremely significant in early examination and monitoring of recurrence of disease after treatment. Biomarkers have expanded great clinical significance owing to their extensive spectra in the identification, elimination, early diagnosis and cure of cancer. In this work, novel, ultrasensitive sandwich-type portable bio device based on citrate-capped silver nanoparticles (Citrate-AgNPs) modified graphene quantum dots (GQDs) nano ink for detection of Prostate specific antigen (PSA) was fabricated. Functionalized cysteamine with gold nanoparticles (Cys-AuNPs) was also utilized to amplify the signal. It provides a good and high external area for the immobilization biotinylated antibody of PSA in the large amount. For the first time, citrate-AgNPs-GQDs nano ink was directly written on the cellulose paper surface (ivory sheet and photographic paper) and modified by Cys-AuNPs. So, final structure of the immunodevices was completed after including of Ab1 and PSA (antigen). The immunosensors were used for the recognition of PSA by using DPVs (differential pulse voltammetry) technique. The obtained low limit of quantification (LLOQ) of the first immunodevice (ivory sheet/Citrate AgNPs-GQDs nano-ink/CysA-Au NPs/Ab1/BSA/PSA/Ab2) was 0.07 μg/L and the linear range for the calibration plot was from 0.07 to 60 μg/L. Also, the achieved LLOQ of the second immunodevice (photographic paper/Citrate AgNPs-GQDs nano-ink/Cys-Au NPs/Ab1/BSA/PSA/Ab2) was 0.05 μg/L with the linear range of 10 to 0.05 μg/L. It is noteworthy that, proposed immunoassay was effectively utilized to the monitoring of PSA glycoprotein in unprocessed human plasma sample. Obtained results show that the constructed immunosensor is able to apply as portable bio device for the clinical analysis of PSA in human plasma samples.

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Section: Introductionmentioning

confidence: 99%

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

Farshchi

Saadati

Hasanzadeh

2020

Heliyon

Self Cite

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“…These processes involve several standard laboratory functions including sample handling, mixing, and reacting of samples and reagents, separation, and detection. LOC can be fabricated on different materials such as glass , silicon , PDMS , PMMA [, paper or a hybrid of PDMS/paper/glass . Presently, thread/PADs can also be fabricated as a simple platform for bioassays .…”

Section: Brief History Of Paper‐based Analytical Devicesmentioning

confidence: 99%

“…Carbohydrate antigen 15‐3 (CA 15‐3) is another biomarker, which is important for screening and diagnosis of breast cancer. Levels of CA 15‐3 (a cut‐off value of 100 U/mL) allow doctors to provide therapy adjustment and to predict breast cancer reoccurrence after curative surgery . Generally, CA 15‐3 levels are determined by quartz crystal microbalance , CLIA , ELISA , sandwich‐type immunosensor , and optical immunoassay .…”

Section: Applications For Biomarker Detectionmentioning

confidence: 99%

“…These techniques are valuable for detection of CA 15‐3, however, demands on rapid and simple immunosensors with low cost and POC capability are on the rise. Hassanpour and co‐workers developed a disposable µPAD, using silver and nanoparticles (AgNPs)‐rGO, as a novel immunosensor for measuring of CA 15‐3 levels . The device was fabricated from nano‐ink technology printing and detection was done electrochemically.…”

Section: Applications For Biomarker Detectionmentioning

confidence: 99%

“…cancer, non‐communicable diseases, and metabolic disorders) relating to current life‐styles of the world population. Tables and describe the target biomarkers, types of paper substrates, fabrication, and detection techniques, analytical performance characteristics with their respective applications in real sample matrices, for tumor marker and other diseased‐related biomarker detection, respectively. Highlights of selected examples are discussed.…”

Section: Applications For Biomarker Detectionmentioning

confidence: 99%

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Recent applications of paper‐based point‐of‐care devices for biomarker detection

Suntornsuk

2019

Electrophoresis

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Detection of biomarkers is essential for diagnosis and prognosis of diseases for healthcare teams to provide timely appropriate treatments. Reliable, inexpensive, and sensitive devices are desirable to serve this purpose. Paper‐based analytical devices (PADs) have gained enormous attention during the past decade as point‐of‐care devices for biomarker detection due to their simplicity, portability, and biocompatibility. Importantly, the devices highly comply with the WHO “ASSURED” concept (i.e. Affordable, Sensitive, Specific, User‐friendly, Rapid and Robust, Equipment free, and Deliverable to end‐users). Thus, PADs could be sustainably alternative tools for biomarker detection, especially in resources‐constraint areas where budget, skillful operators, and health infrastructure are limited. First, this review introduces overviews of biomarkers, their detection and brief history of PADs. Second, description of common fabrication and detection techniques in PADs are provided. Then, it highlights recent state of the art technologies and applications of PADs for various biomarker detection published during 2018 to May 2019. Applications for tumor marker, cardiac and coronary heath disease, and metabolic disorder biomarker detection are tabulated and selected examples are described in details. Finally, it discusses the current challenges, advances, and novel applications of PADs. The review would be valuable for healthcare workers using PADs as cost‐effective point‐of‐care devices for biomarker detection and for researchers working on future developments of the devices.

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Flexible Sensing Systems for Cancer Diagnostics

Brooks

Chakravarty

Yadavalli

2022

Advances in Experimental Medicine and Biology

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A novel paper based immunoassay of breast cancer specific carbohydrate (CA 15.3) using silver nanoparticles-reduced graphene oxide nano-ink technology: A new platform to construction of microfluidic paper-based analytical devices (μPADs) towards biomedical analysis

Cited by 59 publications

References 66 publications

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

A novel immunosensor for the monitoring of PSA using binding of biotinylated antibody to the prostate specific antigen based on nano-ink modified flexible paper substrate: efficient method for diagnosis of cancer using biosensing technology

Recent applications of paper‐based point‐of‐care devices for biomarker detection

Flexible Sensing Systems for Cancer Diagnostics

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