A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays

Egert, Ulrich; Schlosshauer, Bürkhard; Fennrich, Stefan; Nisch, W.; Fejtl, Michael; Knott, Th.; Müller, T.; Hämmerle, H.

doi:10.1016/s1385-299x(98)00013-0

Cited by 220 publications

(156 citation statements)

References 22 publications

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“…of existing microelectrode arrays [5,6,31] has mainly been brane or on an intact membrane displayed a histological tested in terms of electrophysiology, while the histological organization, which by stereomicroscopical inspection and investigations are poor [6] or non-existing [5,31]. In in Nissl and Timm stained sections was similar to cultures general, we found that slice cultures grown on the silicongrown on membranes or by the roller drum method, as based microelectrode arrays displayed few or no differpreviously described [37].…”

Section: Discussionmentioning

confidence: 99%

“…There are existing microelectrode arrays that can be combined with hippocampal slice cultures [5,6,31]. For use with the Physiocard, the slice cultures are grown on a semiporous membrane in an incubator ad modum Stoppini [30] and thereafter transferred to the Physiocard, where the stimulating and recording electrodes are inserted into the cultures from above [31].…”

Section: Introductionmentioning

confidence: 99%

“…2B). Such an Silicon [27], silicon dioxide [29], silicon nitride [6] and arrangement might be appropriate for maintaining the platinum [29], are in general considered to be biocompatcultures between sessions of electrophysiological measureible materials, meaning that the materials do not induce ments, where the chip is mounted in a printed circuit toxic reactions in tissues they are placed in intimate board. The membrane inserts were transferred to 6-well contact with.…”

Section: Introductionmentioning

confidence: 99%

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Biocompatibility of silicon-based arrays of electrodes coupled to organotypic hippocampal brain slice cultures

et al. 2001

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In this study we examined the passive biocompatibility of a three-dimensional microelectrode array (MEA), designed to be coupled to organotypic brain slice cultures for multisite recording of electrophysiological signals. Hippocampal (and corticostriatal) brain slices from 1-week-old (and newborn) rats were grown for 4-8 weeks on the perforated silicon chips with silicon nitride surfaces and 40 mm sized holes and compared with corresponding tissue slices grown on conventional semiporous membranes. In terms of preservation of the basic cellular and connective organization, as visualized by Nissl staining, Timm sulphide silver-staining, microtubule-associated protein 2 (MAP2) and glial fibrillary acidic protein (GFAP) immunostaining, the slice cultures grown on chips did not differ from conventionally grown slice cultures. Neither were there any signs of astrogliosis or neurodegeneration around the upper recording part of the 47-mm-high platinum-tip electrodes. Slice cultures grown on a separate set of chips with platinum instead of silicon nitride surfaces also displayed normal MAP2 and GFAP immunostaining. The width of the GFAP-rich zone (glia limitans) at the bottom surface of the slice cultures was the same (|20 mm) in cultures grown on chips with silicon nitride and platinum surfaces and on conventional insert membranes. The slice cultures grown on chips maintained a normal, subfield differentiated susceptibility to the glutamate receptor agonist N-methyl-D-aspartate (NMDA) and the neurotoxin trimethyltin (TMT), as demonstrated by the cellular uptake of propidium iodide (PI), which was used as a reproducible and quantifiable marker for neuronal degeneration. We conclude that organotypic brain slice cultures can grow on silicon-based three-dimensional microelectrode arrays and develop normally with display of normal subfield differentiated susceptibilities to known excito-and neurotoxins. From this it is anticipated that the set-up, designed for recording of electrophysiological parameters, can be used for long-term studies of defined neuronal networks and provide valuable information on both normal, neurotoxicological and neuropathological conditions. Theme: Disorders of the nervous systemTopic: Neurotoxicity

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biocompatibility of silicon-based arrays of electrodes coupled to organotypic hippocampal brain slice cultures

et al. 2001

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“…Each MEA consisted of a square glass plate (5×5 cm, 1 mm thickness) with a square array of 60 microelectrodes made of titanium nitride at its center (8×8 grid with the corners missing; inter-electrode distance of 200 μm; (Egert, Schlosshauer et al, 1998). The titanium nitride electrodes were flat, circular discs with a diameter of 30 μm and were connected to gold leads that served as contacts for the amplifier head stage.…”

Section: Incubation and Recording Conditionsmentioning

confidence: 99%

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Stewart

Plenz

2008

Journal of Neuroscience Methods

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Cortical networks in vivo and in vitro are spontaneously active in the absence of inputs, generating highly variable bursts of neuronal activity separated by up to seconds of quiescence. Previous measurements in adult rat cortex revealed an intriguing underlying organization of these dynamics, termed neuronal avalanches, which is indicative of a critical network state. Here we demonstrate that neuronal avalanches persist throughout development in cortical slice cultures from newborn rats. More specifically, we find that in spite of large variations of average rate in activity, spontaneous bursts occur with power-law distributed sizes (exponent -1.5) and a critical branching parameter close to 1. Our findings suggest that cortical networks homeostatically regulate a critical state during postnatal maturation.

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“…162,163 Planar MEAs, consisting of 10-100 electrode sites (with diameters on the order of the cells-i.e., ranging from a few microns up to a few tens of microns) spaced at 100-200 μm interdistance on glass plates, were used by Tomas et al, 164 Gross et al, 165 Novak and Wheeler, 166 and many others followed in studies of the activity and plasticity of developing cultured neuronal networks 167,168 or slices. 162,163,[169][170][171][172][173] MEAs are now commercially available in a variety of formats.…”

Section: Extracellular Electrode Arrays For Multisite Recording and Smentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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A novel organotypic long-term culture of the rat hippocampus on substrate-integrated multielectrode arrays

Cited by 220 publications

References 22 publications

Biocompatibility of silicon-based arrays of electrodes coupled to organotypic hippocampal brain slice cultures

Biocompatibility of silicon-based arrays of electrodes coupled to organotypic hippocampal brain slice cultures

Homeostasis of neuronal avalanches during postnatal cortex development in vitro

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

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