A novel neuropeptide, Hym-355, positively regulates neuron differentiation in Hydra

Takahashi, Toshio; Koizumi, Osamu; Ariura, Yuki; Romanovitch, Anna; Bosch, Thomas C. G.; Kobayakawa, Yoshitaka; Yum, Seungshic; Hatta, Masayuki; Fujisawa, Toshitaka

doi:10.1016/s1095-6433(99)90367-7

Cited by 41 publications

(85 citation statements)

References 56 publications

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“…In this context, it is interesting to note that many of these epithelial cell-specific genes are involved in intracellular signaling and signal transduction. Another example of such signaling is the release from epithelial cells of peptide signals regulating nerve cell differentiation (37). Both observations are consistent with the idea that, in multicellular animals, there is cross talk between different cell lineages to maintain constant proportions.…”

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confidence: 68%

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The evolutionary emergence of cell type-specific genes inferred from the gene expression analysis of Hydra

Hwang

Ohyanagi

Hayakawa

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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Cell lineages of cnidarians including Hydra represent the fundamental cell types of metazoans and provides us a unique opportunity to study the evolutionary diversification of cell type in the animal kingdom. Hydra contains epithelial cells as well as a multipotent interstitial cell (I-cell) that gives rise to nematocytes, nerve cells, gland cells, and germ-line cells. We used cDNA microarrays to identify cell type-specific genes by comparing gene expression in normal Hydra with animals lacking the I-cell lineage, so-called epithelial Hydra. We then performed in situ hybridization to localize expression to specific cell types. Eighty-six genes were shown to be expressed in specific cell types of the I-cell lineage. An additional 29 genes were expressed in epithelial cells and were down-regulated in epithelial animals lacking I-cells. Based on the above information, we constructed a database (http://hydra.lab. nig.ac.jp/hydra/), which describes the expression patterns of cell type-specific genes in Hydra. Most genes expressed specifically in either I-cells or epithelial cells have homologues in higher metazoans. By comparison, most nematocyte-specific genes and approximately half of the gland cell-and nerve cell-specific genes are unique to the cnidarian lineage. Because nematocytes, gland cells, and nerve cells appeared along with the emergence of cnidarians, this suggests that lineage-specific genes arose in cnidarians in conjunction with the evolution of new cell types required by the cnidarians.cnidarian ͉ database ͉ epithelial hydra ͉ microarray ͉ nematocyte

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supporting

confidence: 68%

“…Six of them encoded neuropeptides and were expressed in ganglion cells (SI Table 5). Various neuropeptides have been isolated from Hydra by using systematic screening for peptide signaling molecules (37)(38)(39), and four sequences reported in this study encode such known neuropeptides (SI Fig. 3D).…”

mentioning

confidence: 91%

The evolutionary emergence of cell type-specific genes inferred from the gene expression analysis of Hydra

Hwang

Ohyanagi

Hayakawa

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

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“…Hym355 appears to be involved in a positive feedback loop stabilizing the pattern of nerve cells throughout Hydra tissue. A second peptide, Hym33H (AALPW), which is synthesized by epithelial cells, counteracts the effect of Hym355 (Takahashi et al, 2000).…”

Section: Factors Regulating Nerve Cell Differentiationmentioning

confidence: 99%

Interstitial stem cells in Hydra: multipotency and decision-making

David¹

2012

Int. J. Dev. Biol.

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Interstitial stem cells in Hydra constitute a population of multipotent cells, which continuously give rise to differentiated products during the growth and budding of Hydra polyps. They also give rise to germ cells in animals undergoing sexual differentiation. Cloning experiments have shown that interstitial stem cells are multipotent. In vivo tracing of stem cell lineages has revealed that stem cells divide symmetrically to yield two stem cells or asymmetrically to yield one stem cell daughter and one daughter cell which initiates nerve or nematocyte differentiation. Following commitment, some nerve cell precursors migrate from the body column into the head or foot region, thus giving rise to the high density of nerve cells observed in these regions. Stem cell proliferation is regulated by changes in the self-renewal probability and is controlled by stem cell density. Nerve cell commitment is controlled by several peptides including the Head Activator. Factors affecting nematocyte commitment are not known, but wnt and notch signaling are both required for differentiation of committed precursors.

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“…This possibility was the motivation for the Hydra peptide project (Takahashi et al, 1997;Fujisawa, 2008), which had the goal of identifying novel peptides involved in developmental processes in Hydra (Fujisawa and Hayakawa, 2012). This project has led to the identification of the Hym-301 peptide, which is involved in head formation, Hym-355 and the PW peptides, which play stimulatory and inhibitory roles, respectively in nerve cell differentiation, and Hym-346 and Hym-323 peptides, which have roles in foot formation (Grens et al, 1999;Takahashi et al, 2000;Takahashi et al, 2005;. Although Hayakawa et al, (2007) have shown that a computational approach can be used to identify potential precursors to peptides in Hydra in ESTs or in gene models from the Hydra genome, functional screens of the sort developed by the Hydra Peptide Project clearly still have a role to play in defining the molecules that control developmental processes in Hydra, with the genome sequence playing an enabling role.…”

Section: What Genes Are Novel?mentioning

confidence: 99%

The Hydra genome: insights, puzzles and opportunities for Developmental Biologists

Steele

2012

Int. J. Dev. Biol.

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The sequencing of a Hydra genome marked the beginning of a new era in the use of Hydra as a developmental model. Analysis of the genome sequence has led to a number of interesting findings, has required revisiting of previous work, and most importantly presents new opportunities for understanding the developmental biology of Hydra. This review will describe the history of the Hydra genome project, a selection of results from it that are relevant to developmental biologists, and some future research opportunities provided by Hydra genomics.

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A novel neuropeptide, Hym-355, positively regulates neuron differentiation in Hydra

Cited by 41 publications

References 56 publications

The evolutionary emergence of cell type-specific genes inferred from the gene expression analysis of Hydra

The evolutionary emergence of cell type-specific genes inferred from the gene expression analysis of Hydra

Interstitial stem cells in Hydra: multipotency and decision-making

The Hydra genome: insights, puzzles and opportunities for Developmental Biologists

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