A novel necroptosis-related lncRNA signature for predicting prognosis and immune response of colon cancer

Abstract

Background: Numerous lncRNAs have been shown to affect colon cancer (CC) progression, and tumor necroptosis is regulated by several of them. However, the prognostic value of necroptosis-related lncRNA in CC has rarely been reported. In this study, a necroptosis-related lncRNA prognostic model was constructed, which can provide a reference for clinical diagnosis and treatment.Methods: The Cancer Genome Atlas (TCGA) database provided gene expression and lncRNA sequencing data for CC patients, and GSEA provided necroptosis gene data. Differentially expressed necroptosis-related lncRNAs related to prognosis were identified by differential expression analysis, Pearson correlation analysis, and least absolute shrinkage and selection operator (LASSO) regression. Based on the results of the multivariate COX regression analysis, a risk scoring model was constructed, A Kaplan-Meier analysis was performed to compare overall survival (OS) between low-risk and high-risk groups. A nomogram was then developed and validated based on the clinical data and risk scores of CC patients. In addition, Gene Set Enrichment Analysis (GSEA) and immune correlation analysis were conducted to explore the possible pathways and immune regulatory effects of these necroptosis-related lncRNAs.Results: In total, we identified 326 differentially expressed necroptosis-related lncRNAs in the TCGA database. Survival analysis showed that the OS of patients in the low-risk group was significantly better than that in the high-risk group (p < 0.05). Finally, 10 prognostic necroptosis-related lncRNAs were used to construct the nomogram. The composite nomogram prediction model evaluated and validated with good prediction performance (3-year AUC = 0.85, 5-years AUC = 0.82, C-index = 0.78). The GSEA and immune correlation analyses indicated that these lncRNAs may participate in multiple pathways involved in CC pathogenesis and progression.Conclusion: We established a novel necroptosis-related lncRNA CC prognosis prediction model, which can provide a reference for clinicians to formulate personalized treatment and review plans for CC patients. In addition, we also found that these necroptosis-related lncRNAs may affect the pathogenesis and progression of colon cancer through multiple pathways, including altering the activity of various immune cells.

Necroptosis-related lncRNAs signature predicts prognosis and influences immune microenvironment in patients with colon adenocarcinoma

“…Presently, some models based on NRGs can effectively predict immune landscape, therapeutic response, drug sensitivity, and prognosis of patients with COAD [39,40]. Additionally, features based on necroptosis-related miRNA or lncRNA have also been established in COAD [41][42][43]. These models have optimized the survival assessment and individualized management of patients with COAD.…”

Exploring the prognostic significance of iron death‑related lncRNAs in colorectal cancer: A systematic review and meta‑analysis

“…The surge in molecular biology, transcriptomics and genomics research has spotlighted the pivotal role of long non-coding (lnc)RNAs in cancer development, growth and metastasis (7). lncRNAs are more complex than small RNAs and involve multiple regulatory mechanisms, including gene silencing, chromosomal alterations and protein translation regulation (8,9). Specifically, ferroptosis-related lncRNAs have emerged as key players in the pathogenesis if colorectal cancer (10)(11)(12).…”