A novel multidomain transcription coactivator SAYP can also repress transcription in heterochromatin

Shidlovskii, Yulii V.; Краснов, А. Н.; Nikolenko, J. V.; Лебедева, Л. А.; Kopantseva, Marina; Ermolaeva, Maria A.; Ilyin, Yurij V; Набирочкина, Е. Н.; Georgiev, Pavel; Георгиева, С. Г.

doi:10.1038/sj.emboj.7600508

Cited by 43 publications

(61 citation statements)

References 52 publications

(57 reference statements)

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“…Brahma and TFIID are united by coactivator SAYP, which we have described in ref. 13. BTFly includes all subunits of TFIID and Brahma (PBAP subfamily), but not comparably abundant subunits of other coactivators, and is stable in the absence of a chromatin template according to biochemical evidence.…”

Section: Discussionmentioning

confidence: 99%

“…We have described a SAYP gene mutation, e(y)3 u1 , which reduces the level of SAYP (13). Close analysis of males hemizygous for e(y)3 u1 has shown that the viability of hemizygous males is decreased by 20%.…”

Section: Sayp Interacts With Tfiid and Brahma Components In Drosophilamentioning

confidence: 99%

“…4A). SAY was shown to be the minimal domain activating transcription in yeast (13). To ascertain the role of particular SAYP domains in the natural environment, we designed the pTrAssay construct (Fig.…”

Section: Sayp Interacts With Tfiid and Brahma Components In Drosophilamentioning

confidence: 99%

“…SAYP is present at numerous sites on polytene chromosomes and colocalizes with Pol II in transcriptionally active euchromatin. SAYP homologs in various metazoans have an evolutionarily conserved core containing the SAY domain, which is involved in transcription activation, and 2 PHD fingers (13). Recently, SAYP was found to be associated with the chromatinremodeling Brahma complex of the PBAP subfamily (14).…”

mentioning

confidence: 99%

“…We have described the coactivator SAYP in Drosophila (13). SAYP is present at numerous sites on polytene chromosomes and colocalizes with Pol II in transcriptionally active euchromatin.…”

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Transcription coactivator SAYP combines chromatin remodeler Brahma and transcription initiation factor TFIID into a single supercomplex

Vorobyeva

Сошникова

Nikolenko

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

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Transcription activation by RNA polymerase II is a complicated process driven by combined, precisely coordinated action of a wide array of coactivator complexes, which carry out chromatin-directed activities and nucleate the assembly of the preinitiation complex on the promoter. Using various techniques, we have shown the existence of a stable coactivator supercomplex consisting of the chromatin-remodeling factor Brahma (SWI/SNF) and the transcription initiation factor TFIID, named BTFly (Brahma and TFIID in one assembly). The coupling of Brahma and TFIID is mediated by the SAYP factor, whose evolutionarily conserved activation domain SAY can directly bind to both BAP170 subunit of Brahma and TAF5 subunit of TFIID. The integrity of BTFly is crucial for its ability to activate transcription. BTFly is distributed genome-wide and appears to be a means of effective transcription activation.coactivators ͉ protein complex A ctivation of transcription by eukaryotic RNA polymerase II (Pol II) requires different groups of coactivators (for reviews, see refs. 1 and 2). The primary function of coactivators is to remodel and modify the chromatin template. Thus, chromatin remodelers of the Brahma (SWI/SNF-related) family play a genome-wide role in activation of Pol II-transcribed genes (3, 4). One more function of coactivators is to further recruit general transcription factors (GTFs) to form the Pol II preinitiation complex. The TFIID coactivator performs this function for most of Pol II-dependent genes (5, 6).Different coactivators recruited to the promoter assist each other and interact in a highly organized gene-specific manner (for a review, see ref. 7). However, this important regulatory step is still poorly understood. The best studied model is that of successive one-by-one recruitment of coactivators, which, in particular, is confirmed by the fact that the recruitment of chromatin-remodeling complexes is usually a prerequisite for the efficient recruitment of GTFs to the promoter (8, 9). The opposite model proposes one-time recruitment of preexisting supercomplex of several coactivators (10-12), although the composition of such supercomplexes described to date appears to be either ambiguous or incomplete.We have described the coactivator SAYP in Drosophila (13). SAYP is present at numerous sites on polytene chromosomes and colocalizes with Pol II in transcriptionally active euchromatin. SAYP homologs in various metazoans have an evolutionarily conserved core containing the SAY domain, which is involved in transcription activation, and 2 PHD fingers (13). Recently, SAYP was found to be associated with the chromatinremodeling Brahma complex of the PBAP subfamily (14). Here, we show that SAYP interacts both with Brahma and with TFIID, assembling them into a stable supercomplex named BTFly (Brahma and TFIID in one assembly). The presence of all BTFly components is crucial for its function in transcription activation. An important fact is that highly purified BTFly contains the full set of TFIID and Brahma subunits and, t...

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Section: Discussionmentioning

confidence: 99%

Section: Sayp Interacts With Tfiid and Brahma Components In Drosophilamentioning

confidence: 99%

Section: Sayp Interacts With Tfiid and Brahma Components In Drosophilamentioning

confidence: 99%

mentioning

confidence: 99%

“…We have described the coactivator SAYP in Drosophila (13). SAYP is present at numerous sites on polytene chromosomes and colocalizes with Pol II in transcriptionally active euchromatin.…”

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confidence: 99%

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