A novel micropropagation of Lycium ruthenicum and epigenetic fidelity assessment of three types of micropropagated plants in vitro and ex vitro

Gao, Yue; Wang, Qin-Mei; An, Qinxia; Cui, Jianguo; Zhou, Yongbin; Qi, Xinyu; Zhang, Lijie; Li, Lujia

doi:10.1371/journal.pone.0247666

Cited by 7 publications

(3 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although no reports were found on the micropropagation of L. schweinfurthii, it is well studied in other species of the Lycium genus. Multiple shoots and adventitious buds of L. ruthenicum were developed in vitro not only from stems but also from leaf explants [9]. Moreover, the best shoot proliferation of L. depressum was achieved at a low concentration of BA (6-benzyl adenine) and rooted in full-strength MS medium (Murashige and Skoog medium) supplemented with IBA (indole-3-butyric acid) with a high survival rate [10].…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

View full text Add to dashboard Cite

Lycium schweinfurthii is a Mediterranean wild shrub rich in plant secondary metabolites. In vitro propagation of this plant may support the production of valuable dietary supplements for humanity, introduction of it to the world market, and opportunities for further studies. The presented study aimed to introduce an efficient and reproducible protocol for in vitro micropropagation of L. schweinfurthii and assess the genetic stability of micropropagated plants (MiPs) as well as to estimate phenolic, flavonoid, ferulic acid contents, and the antioxidant activity in leaves of micropropagated plants. Two DNA-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), and one biochemical technique, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), were used to assess the genetic stability in MiPs. Spectrophotometric analysis was performed to estimate total phenolic and flavonoid contents and antioxidant activity of MiPs leaves, while ferulic acid content was estimated using high-performance thin-layer chromatography (HPTLC). Sufficient shoot proliferation was achieved at MS (Murashige and Skoog) medium supplemented with 0.4 mg L−1 kinetin and rooted successfully on half-strength MS medium fortified with 0.4 mg L−1 Indole-3-butyric acid (IBA). The Jaccard’s similarity coefficients detected in MiPs reached 52%, 55%, and 82% in the RAPD, ISSR, and SDS-PAGE analyses, respectively. In the dried leaves of MiPs, the phenolic, flavonoid, and ferulic acid contents of 11.53 mg gallic acid equivalent, 12.99 mg catechin equivalent, and 45.52 mg were estimated per gram, respectively. However, an IC50 of 0.43, and 1.99 mg mL−1 of MiP dried leaves’ methanolic extract was required to scavenge half of the DPPH, and ABTS free radicals, respectively. The study presented a successful protocol for in vitro propagation of a valued promising plant source of phenolic compounds.

show abstract

Section: Introductionmentioning

confidence: 99%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

View full text Add to dashboard Cite

show abstract

“…Other factors such as the presence of bioactive compounds in the explants are thought to be antagonistic to the presence of growth regulators, making it difficult to produce plant embryos. Micropropagation including callus induction and subculture have been carried out to obtain plant buds to be developed into plant seeds [23][24] [25]. The micropropagation carried out in this study has shown potential success in producing good quality seeds.…”

Section: Discussionmentioning

confidence: 99%

Micropropagation of Sumatran Frankincense (Styrax Benzoin) Forest Plants Producing Bioactive Raw Materials for Medicine

Nurwahyuni

Situmorang

Sinaga

2023

Advances in Science and Technology

View full text Add to dashboard Cite

Preservation of tropical forest plants that have high economic potential, such as Sumatran benzoin (Styrax benzoin Dryander), is urgently carried out through the provision of quality seeds. The purpose of this study was to propagate the Sumatran Frankincense plant to produce good quality seeds to meet the needs of seeds for forest conservation purposes. The micropropagation technique was carried out using explants from selected parent plants of very good quality. Micropropagation through the use of explants from several parts of the plant aims to produce seeds with the same incense production quality as the parent plant. Explants were selected from the young leaves of the shoots. The experiment was conducted in a completely randomized design with two factors: concentrations of NAA (0-3 mg/L) and BAP (0-3 mg/L). The combination of NAA and BAP in culture media under optimal conditions proved to be effective in inducing callus and plantlet growth. The success of micropropagation has the potential to produce seeds of the same excellent quality as the parent plant in large quantities in a relatively short time. This technique is expected to be able to meet the conservation needs of people's forests and national forests, and in the long term will increase the production of frankincense as a non-timber forest commodity as raw material for medicine.

show abstract

“…Each of the treatments includes five replicates. Cultures were maintained in a growth room with a 16-hour photoperiod under fluorescent light at 25 ± 2ºC (Gao et al, 2021;Vijay, Shukla, & Saxena, 2016). Micro solution 5 mL 5 ml 5 mL 5 mL 5 mL 5 mL 3.…”

Section: Preparation Of Media Using Different Carbon Sourcesmentioning

confidence: 99%

Effect of Plant Growth Regulators, Basal Media Strength and Carbon Sources on Hylocereus Costaricensis (Red Dragon Fruit) Seed Germination

2021

EAJSE

View full text Add to dashboard Cite

Hylocereus costaricensis, commonly known as red dragon fruit or 'pitaya,' is famous for its purple-red flesh. It has achieved international recognition as an ornamental plant and secondly as a commercial fruit crop. Containing high level of vitamin C and antioxidants like betalans, hydroxycinnamates and flavonoids that includes edible fibre with numerous health benefits including weight loss, improve digestion, lower LDL cholesterol, and boost immune system. However, there is no large-scale production of dragon fruit because the seed storage viability is not reliable; the present study aims to employ tissue culture techniques supplied with different plant growth regulators (PGRs), the strength of growth media, and carbon sources. Seed germination was determined by growing the seeds in media enriched with benzyl aminopurine (BAP) 1 mg/L, 2 mg/L BAP, and 15 mg/L gibberellins (GA). Then, the seed growth was measured under different strengths of Murashige and Skoog (MS) basal media, which are full strength, 1/2, 1/4 and 1/8 strength, that promoted the seed germination the most. The percentage of seed germination was determined, and the effects of seed germination was measured by the period of seed to germinate, shoot length, and root length of the plants. In this study, all the dragon fruit seeds were successfully germinated. The seed shows the higher germination is MS + 2 mg/L BAP within 2 days, but the elongation of the shoot is not the fastest. The seed germination is effective in 1/8 media strength on day 2; the seeds are already germinated. However, the elongation for roots and shoots is the best in 1/2 media strength. The effect of carbon sources influences the fastest day to germinate, demonstrated in sucrose 30 g/L but not for root and shoot length. The 30 g/L glucose enhances the root length the highest, which is 2.48 cm. The average shoot length shows in TCS0 and TCS3 was similar, which was 1.38 cm. The effect of carbon sources that boost the shoot length the most is in TCS3 (2.04 cm), fructose 15 g/L. Those factors should be considered to enhance the best condition for dragon fruit planting and achieve the large-scale production,

show abstract

A novel micropropagation of Lycium ruthenicum and epigenetic fidelity assessment of three types of micropropagated plants in vitro and ex vitro

Cited by 7 publications

References 51 publications

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Micropropagation of Sumatran Frankincense (Styrax Benzoin) Forest Plants Producing Bioactive Raw Materials for Medicine

Effect of Plant Growth Regulators, Basal Media Strength and Carbon Sources on Hylocereus Costaricensis (Red Dragon Fruit) Seed Germination

Contact Info

Product

Resources

About