A novel method for the rapid detection of benzo(a)pyrene in liquid milk by dimethyl sulfoxide selectively enhanced synchronous fluorescence spectrometry

Abstract

Based on the high solubility efficiency and strong fluorescence response of benzo(a)pyrene (BaP) in dimethyl sulfoxide in combination with the high-performance derivative constant-energy synchronous fluorescence spectroscopic (DCESFS) technique, a simple, sensitive and economic method was developed for the determination of BaP in liquid milk. This method comprises ultrasound-assisted solvent extraction, solvent replacement and DCESFS detection. No saponification or other tedious clean-up procedures were needed. The recoveries of BaP in different milk samples were greater than 82%. Detection limits in full- and low-fat milk were 0.03 and 0.04 μg kg(-1), respectively.

Rapid determination of benzo[a]pyrene by membrane enrichment coupled with solid‐phase constant‐wavelength synchronous fluorescence spectrometry

“…Synchronous fluorescence spectrometry (SFS) has been developed for the simultaneous analysis of multi‐component samples without pre‐separation. Studies have shown that SFS can avoid interference between different PAHs . Conventional SFS maintains a constant‐wavelength differential (Δλ) between excitation and emission monochromators, as each is scanned through the spectral region of interest.…”