A novel method for determining articular cartilage chondrocyte mechanics in vivo

Abusara, Ziad; Seerattan, Ruth A.; Leumann, André; Thompson, R. I.; Herzog, Walter

doi:10.1016/j.jbiomech.2010.11.031

Cited by 44 publications

(46 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vivo measurement of cartilage and chondrocyte mechanics in human subjects, where the joints are exposed to loadings of daily activities, is not necessarily within the reach of the current state of the art. Recent experimentation illustrated the possibility to quantify cartilage and chondrocyte deformations of fresh cadaveric samples in situ [10] and of animals in vivo [11], through invasive approaches. Modelling and simulation provide a means for in-depth exploration of such data through descriptive analysis [12].…”

Section: Background and Motivationmentioning

confidence: 99%

“…Yet, an understanding of multi-modal loading and overall deformations of individual cells and/or populations of cells may be necessary. Metrics such as cell height and width, aspect ratio and volume (undeformed and deformed) have commonly been used for describing experimental data [11] and simulation results [56], with the intention to associate cell mechanics with cell damage and mechanical stimuli. Such descriptors, however, are not readily available in raw simulation results, and automated procedures are necessary if these variables need to be evaluated for hundreds of cells and for a large number of simulation conditions.…”

Section: Post-processingmentioning

confidence: 99%

See 1 more Smart Citation

Multiscale cartilage biomechanics: technical challenges in realizing a high-throughput modelling and simulation workflow

et al. 2015

View full text Add to dashboard Cite

show abstract

Section: Background and Motivationmentioning

confidence: 99%

Section: Post-processingmentioning

confidence: 99%

Multiscale cartilage biomechanics: technical challenges in realizing a high-throughput modelling and simulation workflow

et al. 2015

View full text Add to dashboard Cite

show abstract

“…The left knee joints were shaved and secured in a stereo-taxic frame built onto the stage of a dissecting microscope. The medial aspect of the joint was exposed with a 6 mm incision just posterior to the medial collateral ligament (Abusara et al, 2011).…”

Section: Animal Preparationmentioning

confidence: 99%

“…Recently, we developed methods to quantify the bone, cartilage, and chondrocyte mechanics in the intact knee joint of live mice loaded by controlled muscular contractions (Abusara et al, 2011). Simultaneously, we can also measure selected generic chondrocyte signalling events, such as intracellular calcium ''sparks'' (Han et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Muscular loading of joints triggers cellular secretion of PRG4 into the joint fluid

Abusara

Krawetz

Steele

et al. 2013

Journal of Biomechanics

Self Cite

View full text Add to dashboard Cite

We developed a novel testing system that allows quantification of joint loading and permits analysis of changes in total protein and PRG4 contents in joint fluid of intact knees in live mice. A sequence of 15 repeat, isometric muscular contractions of "low" intensity (less than 50% of the maximal isometric muscular force), and "high" intensity (greater than 55% of maximal) were applied repeatedly (up to five times with a 15 min rest between contractions) to the mouse knee. Increases in knee joint loading were accompanied with significant increases in total protein (p<0.0001) and PRG4 concentrations in the synovial fluid. Total protein and PRG4 concentrations decreased with repeated "high" intensity loading. However, the addition of cell secretion inhibitors to the knee prior to muscular loading resulted in PRG4 levels that remained below the detection limit for all loading conditions. These results suggest that changes in synovial fluid proteins and PRG4 concentrations upon joint loading are mediated by cells within the joint, and that these changes may be used as quantitative indicators for the intensity and duration of acute joint loading, and might serve as a powerful clinical tool to assess the effectiveness of rehabilitation and prevention exercise programs.

show abstract

“…The latter destabilize the joint, altering mechanics, and thus presumably damaging joint surfaces by abnormal loads on their surfaces. [7][8][9] In models that cause mechanical abnormalities along with biological changes, separating the potential damaging effects of the biological changes alone have not been attempted. We created a surgical model of OA in which we could investigate the isolated effects of injury-induced inflammation without destabilizing the knee.…”

mentioning

confidence: 99%

New surgical model of post‐traumatic osteoarthritis: Isolated intra‐articular bone injury in the rabbit

Huebner

Shrive

Frank

2013

Journal Orthopaedic Research

View full text Add to dashboard Cite

Osteoarthritis (OA) is a leading cause of disability worldwide. We hypothesized that inflammation following isolated intraarticular bone injury can stimulate post-traumatic OA and developed a rabbit model to test that concept. Sixty female New Zealand White Rabbits were used. Twenty-six experimental animals had two holes drilled into their right femoral-notch, 18 rabbits had sham surgery, and 16 were un-operated controls. Rabbits were euthanized in subgroups at 72 h, 3, 6, 9, and 52 weeks. Knees were assessed grossly and tissues collected. Cartilage and synovium were analyzed with histology and qPCR and subgroups compared statistically. All surgical joints showed gross and histological (modified Mankin score) cartilage damage after surgery, with experimentals worsening with time (p < 0.05). Cartilage qPCR showed fivefold increases in TGFb (p < 0.05) expression at 72 h and 3 weeks with sixfold increases in MMP13 (p < 0.025) expression at 72 h. By 6 weeks, expression of these markers was similar to baseline levels. Synovial membrane thickening with increased cellularity was seen at both 9 and 52 weeks (p < 0.05). Short-term synovial inflammatory marker (IL-1b, IL-Ra, IL-6, and IL-8) expression was three-to fourfold increase in experimentals at 72 h (p < 0.01) returning to baseline levels by 3 weeks. Intra-articular bone injury creates early joint inflammation with some chronic synovial changes and progressive cartilage damage consistent with OA in adult rabbits. This model provides an exciting new avenue to potentially explore some relevant inflammatory drivers of OA without major mechanical variables. ß

show abstract

A novel method for determining articular cartilage chondrocyte mechanics in vivo

Cited by 44 publications

References 21 publications

Multiscale cartilage biomechanics: technical challenges in realizing a high-throughput modelling and simulation workflow

Multiscale cartilage biomechanics: technical challenges in realizing a high-throughput modelling and simulation workflow

Muscular loading of joints triggers cellular secretion of PRG4 into the joint fluid

New surgical model of post‐traumatic osteoarthritis: Isolated intra‐articular bone injury in the rabbit

Contact Info

Product

Resources

About