A Novel Laser Fiberscope for Simultaneous Imaging and Phototherapy of Peripheral Lung Cancer

Kinoshita, Tomonari; Effat, Andrew; Gregor, Alexander; Ishiwata, Tsukasa; Motooka, Yamato; Ujiie, Hideki; Wilson, Brian C.; Zheng, Gang; Weersink, Robert A.; Asamura, Hisao; Yasufuku, Kazuhiro

doi:10.1016/j.chest.2019.04.010

Cited by 18 publications

(8 citation statements)

References 17 publications

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“…However, it is worth noting that the number of image pixels obtained is similar to a 10,000 elements fiber bundle, which has been widely used for miniature endoscopes. 27…”

Section: Optical and Scanning System Configurationmentioning

confidence: 99%

Triple-modality co-registered endoscope featuring wide-field reflectance imaging, and high-resolution multiphoton and optical coherence microscopy

Vega

Galvez

Romano

et al. 2021

J. Optical Microsystems

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We present the design and feasibility testing of a multimodal co-registered endoscope based on a dual-path optical system integrated with a scanning piezo. This endoscope incorporates three different imaging modalities. A large field-of-view (FOV) reflectance imaging system enables visualization of objects several millimeters in front of the endoscope, while optical coherence microscopy (OCM) and multiphoton microscopy (MPM) are employed in contact with tissue to further analyze suspicious areas. The optical system allows multiple different imaging modalities by employing a dual optical path. One path features a low numerical aperture (NA) and wide FOV to allow reflectance imaging of distant objects. The other path features a high NA and short working distance to allow microscopy techniques such as OCM and MPM. Images of test targets were obtained with each imaging modality to verify and characterize the imaging capabilities of the endoscope. The reflectance modality was demonstrated with a 561 nm laser to allow high contrast with blood vessels. It achieved a lateral resolution of 24.8 μm at 5 mm and a working distance from 5 to 30 mm. OCM was performed with a 1300 nm super-luminescent diode since this wavelength experiences low relative scattering to allow for deeper tissue imaging. Measured OCM lateral and axial resolution was 4.0 and 14.2 μm, respectively. MPM was performed with a custom 1400 nm femtosecond fiber laser, a wavelength suitable for exciting multiple exogenous, and some endogenous fluorophores, as well as providing information on tissue composition through harmonic generation processes. A 4.0 × μm MPM lateral resolution was measured.

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“…However, it is worth noting that the number of image pixels obtained is similar to a 10,000 elements fiber bundle, which has been widely used for miniature endoscopes. 27…”

Section: Optical and Scanning System Configurationmentioning

confidence: 99%

Triple-modality co-registered endoscope featuring wide-field reflectance imaging, and high-resolution multiphoton and optical coherence microscopy

Vega

Galvez

Romano

et al. 2021

J. Optical Microsystems

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“…Porphysomes were synthesized as previously reported (24). For fluorescence imaging, laser excitation was performed at 50 mW using a 671-nm source (22). Fluorescence images were acquired through a 700-nm long-pass filter.…”

Section: P D T P T T ) W E H a V E P R E V I O U S L Y D E S C R I B E D ( 2 3 )mentioning

confidence: 99%

“…We have previously reported on an ultrasmall parallel-type composite optical fiberscope (COF) with a 0.97-mm outer diameter tip that was developed in collaboration with OK Fiber Technology (Kyoto, Japan). Its small size and tip flexibility enable insertion into ultrasmall airways (22). The integration of illumination and laser irradiation fibers inside the COF allows simultaneous white-light and fluorescence imaging, as well as real-time monitoring of tip position during laser phototherapy.…”

Section: Introductionmentioning

confidence: 99%

A preclinical research platform to evaluate photosensitizers for transbronchial localization and phototherapy of lung cancer using an orthotopic mouse model

Ishiwata¹,

Seki²,

Gregor³

et al. 2021

Transl Lung Cancer Res

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Background: Establishing the efficacy of novel photosensitizers (PSs) for phototherapy of lung cancer requires in vivo study prior to clinical evaluation. However, previously described animal models are not ideal for assessing transbronchial approaches with such PSs.Methods: An ultra-small parallel-type composite optical fiberscope (COF) with a 0.97 mm outer diameter tip. The integration of illumination and laser irradiation fibers inside the COF allows simultaneous whitelight and fluorescence imaging, as well as real-time monitoring of tip position during laser phototherapy. An orthotopic lung cancer mouse model was created with three human lung cancer cell lines transbronchially inoculated into athymic nude mice. The COF was inserted transbronchially into a total of 15 mice for tumor observation. For in vivo fluorescence imaging, an organic nanoparticle, porphysome, was used as a PS. Laser excitation through the COF was performed at 50 mW using a 671 nm source.Results: The overall success rate for creating orthotopic lung tumors was 71%. Transbronchial white light images were successfully captured by COF. Access to the left main bronchus was successful in 87% of mice (13/15), the right main bronchus to the cranial lobe bronchus level in 100% (15/15), and to the right basal trifurcation of the middle lobe, caudal lobe and accessory lobe in 93% (14/15). For transbronchial tumor localization of orthotopic lung cancer tumors, PS-laden tumor with the strong signal was clearly contrasted from the normal bronchial wall. Conclusions:The ultra-small COF enabled reliable transbronchial access to orthotopic human lung cancer xenografts in vivo. This method could serve as a versatile preclinical research platform for PS evaluation in lung cancer, enabling transbronchial approaches in in vivo survival models inoculated with human lung cancer cells.

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“…Estas complicações associadas ao compromisso funcional respiratório e baixa reserva cardiovascular em muitos doentes, tornam a terapêutica broncoscópica de ablação de tumores periféricos apelativa, contudo, para ser alcançada três premissas têm de se verificar: localização exata da lesão periférica; confirmação de que o tumor é alcançável; e capacidade para realizar um tratamento eficaz e seguro por broncoscopia. Os desenvolvimentos nestes últimos anos têm-se focado em responder a estes desafios com melhoria das plataformas de orientação e localização, anteriormente abordadas, desenvolvimento de novos métodos de tratamento ou adaptação de terapêuticas utilizadas a nível da via aérea central para a periferia 45 .…”

Section: Terapêutica Broncoscópica: Onde Estamos E Para Onde Vamos?unclassified

Papel da broncologia no cancro do pulmão: (R)evolução nos últimos 20 anos

Bugalho¹,

Guedes²

2020

gecp

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A Novel Laser Fiberscope for Simultaneous Imaging and Phototherapy of Peripheral Lung Cancer

Cited by 18 publications

References 17 publications

Triple-modality co-registered endoscope featuring wide-field reflectance imaging, and high-resolution multiphoton and optical coherence microscopy

Triple-modality co-registered endoscope featuring wide-field reflectance imaging, and high-resolution multiphoton and optical coherence microscopy

A preclinical research platform to evaluate photosensitizers for transbronchial localization and phototherapy of lung cancer using an orthotopic mouse model

Papel da broncologia no cancro do pulmão: (R)evolução nos últimos 20 anos

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