A novel lab-on-chip platform with integrated solid phase PCR and Supercritical Angle Fluorescence (SAF) microlens array for highly sensitive and multiplexed pathogen detection

Hung, Tran Quang; Chin, Wai Hoe; Sun, Yi; Wolff, Anders; Bang, Dang Duong

doi:10.1016/j.bios.2016.11.028

Cited by 41 publications

(31 citation statements)

References 20 publications

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“…According to the law of refraction (Snell's law), the critical angle for the two media in our investigation (air, n 1 = 1, and 12 see Fig. S2 in the ESI † for the measurement of COC refractive index.…”

Section: Modelling Of the Optimal Height Of The Saf Structuresmentioning

confidence: 99%

“…The limit of detection (LOD) was determined at a SNR of 3. 18 All the experiments were performed in triplicate.…”

Section: Experiments On the Fabricated Chipsmentioning

confidence: 99%

“…Instead of using millimetre-range solid paraboloid elements, our group has developed a technique to fabricate micro cone-shaped SAF arrays on which multiplexed detection of pathogens with high sensitivity and a low volume of reagents were achieved. 8,12 The detection technique is carried out based on two portions: (i) using solid-phase PCR and (ii) fabrication of micro SAF arrays. In the fabrication step of micro SAF arrays, the size optimisation of the SAF structures, however, has not yet been conducted, which gives rise to limitations of and obstacles in this method in massive chip production for industrial applications, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Optimising the supercritical angle fluorescence structures in polymer microfluidic biochips for highly sensitive pathogen detection: a case study on Escherichia coli

et al. 2019

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Section: Modelling Of the Optimal Height Of The Saf Structuresmentioning

confidence: 99%

“…The limit of detection (LOD) was determined at a SNR of 3. 18 All the experiments were performed in triplicate.…”

Section: Experiments On the Fabricated Chipsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Optimising the supercritical angle fluorescence structures in polymer microfluidic biochips for highly sensitive pathogen detection: a case study on Escherichia coli

et al. 2019

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“…Especially for plant samples, liquid nitrogen needs during grinding, and the entire process is time consuming and complicated. Exploiting microfluidic chips in nucleic acid analysis can greatly reduce reagent consumption, shorten assay time and realize precise temperature [18,19]. In the recent decades, microfluidic chips for nucleic acid analysis are mainly concentrated in biomedicine and pointof-care test (POCT) [20,21], but there are few studies in plants, especially in the detection of mutations.…”

Section: Introductionmentioning

confidence: 99%

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

Zhu

et al. 2019

Biotechnology & Biotechnological Equipment

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Rice is a crucial crop to meet global food demands, therefore, it is necessary to develop rapid, efficient and inexpensive methods for screening mutants of important genes in molecular breeding. In this research, a simple microfluidic chip-integrated one-step polymerase chain reaction (PCR) and high-resolution melting (HRM) analysis were designed for the rapid screening of rice mutants. In order to complete the above processes, we also installed a self-developed functional instrument integrated with a temperature control system and a fluorescence detection department. This microfluidic platform was validated by analysing genes related to insect resistance, phenotype and yield of two important varieties of rice, Oryza sativa L. ssp. japonica. and Oryza sativa L. ssp. indica (93-11). The results showed that the platform could detect singlenucleotide mutations between the wild and the mutant rice plants in 1 h and the accuracy was consistent with gene sequencing regarded as the golden standard of the mutants screening. Compared to the traditional method (requiring approximately 3 h), this platform can distinguish mutants from wild types more rapidly. This system is a simple, integrated, disposable and lowcost tool for high-throughput screening of mutants in molecular breeding. More important, this portable instrument can be used as a point-of-care platform for nucleic acid diagnostics in many scientific fields. ARTICLE HISTORY

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“…Obtaining quantitative answers is generally considered challenging, since it often requires complex and expensive electronics and electrical read-out systems and sensors. This in turn raises the cost, hence opposing the realization of PoC devices [ 10 ]. Now, however, we believe that a lot of the potential difficulties and obstacles for obtaining electronic control and read-out have been addressed, and to an increasingly larger extent, solved, in the last few years, due to the rise of the open source community, especially within open source hardware [ 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

From Lab on a Chip to Point of Care Devices: The Role of Open Source Microcontrollers

et al. 2018

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Microcontrollers are programmable, integrated circuit chips. In the last two decades, their applications to industrial instruments, vehicles, and household appliances have reached the extent that microcontrollers are now the number-one selling electronic chip of all kinds. Simultaneously, the field of lab-on-a-chip research and technology has seen major technological leaps towards sample handling, sample preparation, and sensing for use in molecular diagnostic devices. Yet, the transformation from a laboratory based lab-on-a-chip technology to actual point-of-care device products has largely been limited to a fraction of the foreseen potential. We believe that increased knowledge of the vast possibilities that becomes available with open source microcontrollers, especially when embedded in easy-to-use development environments, such as the Arduino or Raspberry Pi, could potentially solve and even bridge the gap between lab-on-a-chip technology and real-life point of care applications. The profuse availability and extraordinary capabilities of microcontrollers, namely within computation, communication, and networking, combined with easy-to-use development environments, as well as a very active and fast moving community of makers, who are eager to share their knowledge, could potentially be the difference between a dreadful “chip-in-a-lab”-situation, and the next successful start-up. Here follows a brief insight into how open source microcontrollers could potentially have a transformative effect on the field of lab-on-a-chip research and technology. Details in some specific areas of application are briefly treated before addressing challenges and future perspectives.

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A novel lab-on-chip platform with integrated solid phase PCR and Supercritical Angle Fluorescence (SAF) microlens array for highly sensitive and multiplexed pathogen detection

Cited by 41 publications

References 20 publications

Optimising the supercritical angle fluorescence structures in polymer microfluidic biochips for highly sensitive pathogen detection: a case study on Escherichia coli

Optimising the supercritical angle fluorescence structures in polymer microfluidic biochips for highly sensitive pathogen detection: a case study on Escherichia coli

A microfluidic system integrated one-step PCR and high-resolution melting analysis for rapid rice mutant detection

From Lab on a Chip to Point of Care Devices: The Role of Open Source Microcontrollers

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