A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells

Chiou, Shih Hwa; Kao, Chung Lan; Peng, Chi Hsien; Chen, Shih‐Jen; Tarng, Yih-Wen; Ku, Hung Hai; Chen, Yu‐Chih; Shyr, Yi Ming; Liu, Ru‐Shi; Hsu, Chien Jen; Yang, De‐Ming; Hsu, Wen Ming; Kuo, Cheng Deng; Lee, Chen Hsen

doi:10.1016/j.bbrc.2004.11.061

Cited by 62 publications

(40 citation statements)

References 28 publications

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“…In accordance with Andreas et al [8,9] , under serum-free medium with EGF and bFGF for 14-day culture, BMSCs could aggregate into neuron spheres-like bodies and expressed high level of nestin protein. Maybe growth factors EGF and bFGF played an important role in differentiation.…”

Section: Discussionsupporting

confidence: 88%

In vitro culture of bone marrow mesenchymal stem cells in rats and differentiation into retinal neural-like cells

Sun

Jiang

Yang

2007

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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In order to study the in vitro culture and expansion of bone marrow mesenchymal stem cells in rats (rMSCs) and the possibility of rMSCs differentiation into retinal neural cells, the bone marrow-derived cells in SD rats were isolated and cultured in vitro. The retinal neural cells in SD rats were cultured and the supernatants were collected to prepare conditioned medium. The cultured rMSCs were induced to differentiate by two steps. Immunofluorescence method and anti-nestin, anti-NeuN, anti-GFAP and anti-Thy1.1 antibodies were used to identify the cells derived from the rMSCs. The results showed that the in vitro cultured rMSCs grew well and expanded quickly. After induction with two conditioned media, rMSCs was induced to differentiate into neural progenitor cells, then into retinal neural-like cells which were positive for nestin, NeuN, GFAP and Thy1.1 detected by fluorescence method. The findings suggested that rMSCs could be culture and expanded in vitro, and induced to differentiate into retinal neural-like cells.

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Section: Discussionsupporting

confidence: 88%

In vitro culture of bone marrow mesenchymal stem cells in rats and differentiation into retinal neural-like cells

Sun

Jiang

Yang

2007

J. Huazhong Univ. Sci. Technol. [Med. Sci.]

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“…Recently researches showed that BMSC don't express MHC class II antigens, co-stimulatory molecules CD80, CD 84 or CD40 and only express low quantity of MHC class I antigens, so they can be invisible for recipient immune system. This makes MSCs universal also forallo-and even xeno-transplantations (Chiou et al, 2005). It was also shown that MSC itself suppresses immune system by suppressing T-cell, Bcell and NK activity (Tse et al, 2003;Ribeiro et al, 2013).…”

Section: Bone Marrow-derived Mesenchymal Stem Cellsmentioning

confidence: 98%

Mesenchymal Stem Cell Transplantation for Retinal Degenerations and Dystrophies: Present and Future

Ghazaryan¹,

Wang²,

Zhang³

et al. 2014

American Journal of Biochemistry and Biotechnology

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Retinal degenerations are the main causes of irreversible blindness in developed countries. Up to date the main pathological mechanisms of these diseases are not fully understood and consequently there is no complete treatment option for those diseases. In this aspect stem cells have drawn attention of many researchers and health care professionals. Considering ethical issues, safety and facile isolation Mesenchymal Stem Cells (MSCs) are more preferable for practical use. They have been used for several preclinical and clinical trials. In general the results were promising, however broader practical use should be preceded by resolving many problems and questions. In this review we will describe mesenchymal stem cells, especially those derived from Bone-Marrow (BMSC), their main features, privilege, mechanisms of action and their potential use for the treatment of retinal degenerations. We will also discuss the results of several pre-clinical and clinical trials.

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“…For example, Yang et al reported that human BMSCs differentiated into neural-like cells expressing special neuronal markers, and the expression of these markers was significantly increased by co-culturing with a bovine retinal extract [32]. Chiou et al found that co-culturing with RPE and subsequent culturing with a neurogenic selection medium for two weeks facilitated the photoreceptor-lineage differentiation of hBMSCs [22]. BMSCs were also shown to differentiate to photoreceptor-like cells in an in vitro model by RPE-conditioned medium treatment [33].…”

Section: Discussionmentioning

confidence: 99%

“…After the eighth passage, hBMSCs were induced to undergo osteogenic, adipogenic, or chondrogenic differentiation as previously described [22]. To stimulate osteogenic differentiation, cells were treated with osteogenic medium for three weeks.…”

Section: Methodsmentioning

confidence: 99%

“…The microenvironment consists of many factors critically involved in the differentiation of stem cells [20,21]. Replicating the local environment offers a potentially successful way of mimicking the physiological conditions necessary to induce BMSC differentiation [22,23]. Our previous results demonstrated that BMSCs could be induced to express retinal neuron-specific phenotypic markers in response to retinal neuron co-culture using a transwell system [24].…”

Section: Introductionmentioning

confidence: 99%

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Human Bone Marrow Stromal Cells can Differentiate to a Retinal Pigment Epithelial Phenotype when Co-Cultured with Pig Retinal Pigment Epithelium using a Transwell System

Duan¹,

Xu²,

Zeng³

et al. 2013

Cell Physiol Biochem

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Background: There is an increasing interest in generating retinal pigment epithelial (RPE) cells from stem cells for therapy against degenerative eye diseases. Human bone marrow stromal cells (hBMSCs) can be induced to express retinal neuron-specific markers when co-cultured with retinal neurons, however, whether hBMSCs can differentiate into RPE-like cells in a co-culture system has not been clarified. Methods: The induction of hBMSCs into RPE-like cells was performed by combining hBMSCs and pig RPE cells in a transwell system. The biomarkers of hBMSCs-derived RPE cells were determined by quantitative RT-PCR and immunofluorescence. The function of induced cells was assayed by ELISA for secretion of neurotrophic factors. Results: Intracellular pigment granules and many RPE markers existed in hBMSCs-derived RPE cells after co-culturing with pig RPE cells for 14 days. Typical RPE functions, such as phagocytosis of photoreceptor outer segments and secretion of the trophic factors, brain-derived neurotrophic factor (BDNF) and glia-derived neurotrophic factor (GDNF), were observed in these induced cells. Conclusion: hBMSCs can be induced toward functional RPE cells simply by transwell-based co-culture with RPE cells.

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A novel in vitro retinal differentiation model by co-culturing adult human bone marrow stem cells with retinal pigmented epithelium cells

Cited by 62 publications

References 28 publications

In vitro culture of bone marrow mesenchymal stem cells in rats and differentiation into retinal neural-like cells

In vitro culture of bone marrow mesenchymal stem cells in rats and differentiation into retinal neural-like cells

Mesenchymal Stem Cell Transplantation for Retinal Degenerations and Dystrophies: Present and Future

Human Bone Marrow Stromal Cells can Differentiate to a Retinal Pigment Epithelial Phenotype when Co-Cultured with Pig Retinal Pigment Epithelium using a Transwell System

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