A Novel Grape-Derived Prebiotic Selectively Enhances Abundance and Metabolic Activity of Butyrate-Producing Bacteria in Faecal Samples

Oliver, Lia; Ramió‐Pujol, Sara; Amoedo, Joan; Malagón, Marta; Serrano, Marta; Bahí, Anna; Lluansí, Aleix; Torrealba, Leyanira; Busquets, David; Pardo, Laura; Serra‐Pagès, Mariona; Aldeguer, Xavier; Garcia‐Gil, L. J.

doi:10.3389/fmicb.2021.639948

Cited by 3 publications

(1 citation statement)

References 55 publications

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“…accompanied by increased 4-hydroxybutyrate appeared to reduce butyrate production (Chiu et al, 2019). Butyrate, a type of SCFA, plays a trophic role as a nutrient for colonocytes and enhances the repair of the injured gut epithelium in IBD (Oliver et al, 2021). This suggests that 4hydroxybutyrate might exhibit an adverse effect on the tissue repair of epithelial cells.…”

Section: Discussionmentioning

confidence: 98%

Gut Microbiota and Metabolite Changes in Patients With Ulcerative Colitis and Clostridioides difficile Infection

Wan

Zhang

et al. 2022

Front. Microbiol.

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BackgroundPatients with ulcerative colitis (UC) are at an increased risk of developing Clostridioides difficile infection (CDI), which in turn leads to poor outcomes. The gut microbial structure and metabolites in patients with UC and CDI have been scarcely studied. We hypothesized that CDI changes the gut microbiota and metabolites of patients with UC.Materials and MethodsThis study included 89 patients: 30 healthy controls (HC group), 29 with UC alone (UCN group), and 30 with UC and CDI (UCP group). None of the participants has been exposed to antibiotic treatments during the 3 months before stool collection. Stool samples were analyzed using 16S rRNA gene sequencing of the V3–V4 region and gas chromatography tandem time-of-flight mass spectrometry.ResultsThe UCN group displayed lower diversity and richness in gut microbiota and a higher relative abundance of the phylum Proteobacteria than the HC group. There were no significant differences between the UCN and UCP groups in the α-diversity indices. The UCP group contained a higher relative abundance of the genera Clostridium sensu stricto, Clostridium XI, Aggregatibacter, and Haemophilus, and a lower relative abundance of genera Clostridium XIVb and Citrobacter than the UCN group. In the UCN group, the increased metabolites included putrescine, maltose, 4-hydroxybenzoic acid, 4-hydroxybutyrate, and aminomalonic acid. Spearman’s correlation analysis revealed that these increased metabolites negatively correlated with Clostridium XlVb and positively correlated with the four enriched genera. However, the correlations between hemoglobin and metabolites were contrary to the correlations between erythrocyte sedimentation rate and high-sensitivity C-reactive protein and metabolites.ConclusionOur study identified 11 differential genera and 16 perturbed metabolites in patients with UC and CDI compared to those with UC alone. These findings may guide the design of research on potential mechanisms and specific treatments for CDI in patients with UC.

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Section: Discussionmentioning

confidence: 98%