A novel gene, CaATHB-12, negatively regulates fruit carotenoid content under cold stress in Capsicum annuum

Zhang, Rui-Xing; Zhu, Wenchao; Cheng, Guo-Xin; Yu, Yanan; Li, Quanhui; Haq, Saeed Ul; Said, Fazal; Gong, Zhen-Hui

doi:10.29219/fnr.v64.3729

Cited by 13 publications

(10 citation statements)

References 99 publications

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“…Pepper ( Capsicum annuum L.) is an economically important vegetable crop worldwide for its high contents of many essential nutrients. However, its growth and development are usually negatively influenced by various stresses, causing a serious loss of its yield and quality ( Guo et al, 2015 ; Zhang R.X. et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Phytochrome-Interacting Factor Genes in Pepper and Functional Analysis of CaPIF8 in Cold and Salt Stress

et al. 2021

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As a subfamily of basic helix-loop-helix (bHLH) transcription factors, phytochrome-interacting factors (PIFs) participate in regulating light-dependent growth and development of plants. However, limited information is available about PIFs in pepper. In the present study, we identified six pepper PIF genes using bioinformatics-based methods. Phylogenetic analysis revealed that the PIFs from pepper and some other plants could be divided into three distinct groups. Motif analysis revealed the presence of many conserved motifs, which is consistent with the classification of PIF proteins. Gene structure analysis suggested that the CaPIF genes have five to seven introns, exhibiting a relatively more stable intron number than other plants such as rice, maize, and tomato. Expression analysis showed that CaPIF8 was up-regulated by cold and salt treatments. CaPIF8-silenced pepper plants obtained by virus-induced gene silencing (VIGS) exhibited higher sensitivity to cold and salt stress, with an obvious increase in relative electrolyte leakage (REL) and variations in the expression of stress-related genes. Further stress tolerance assays revealed that CaPIF8 plays different regulatory roles in cold and salt stress response by promoting the expression of the CBF1 gene and ABA biosynthesis genes, respectively. Our results reveal the key roles of CaPIF8 in cold and salt tolerance of pepper, and lay a solid foundation for clarifying the biological roles of PIFs in pepper and other plants.

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Section: Introductionmentioning

confidence: 99%

Characterization of Phytochrome-Interacting Factor Genes in Pepper and Functional Analysis of CaPIF8 in Cold and Salt Stress

et al. 2021

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“…62 An HD-ZIP I family TF CaATHB-12 regulates the accumulation of carotenoids in C. annuum fruits and is responsive to cold stress. 63 MATE family genes such as CaMATE02, CaMATE05, CaMATE12, CaMATE25, and CaMATE30 show differential expression during green and red fruit ripening stage and may be involved in biosynthesis of different secondary metabolites in C. annuum. 64 Transcriptomic analysis of red and orange pepper fruits revealed that the differential regulation of CCS gene via F-box SKIP23, GATA TF 26, U-Box domain containing protein 52, zinc finger family FYVE/ PHD-type, RING/FYVE/PHD-type, and CONSTANS-Like 9 TFs results in different colors of the pepper fruit.…”

Section: Capsaicinoidsmentioning

confidence: 99%

“…129 Cold stress induced carotenoid biosynthesis via up regulating the gene expression of carotenoid biosynthesis genes such as LCYB, PSY, ZEP, and β-carotene hydroxylase in CaATHB-12 silenced C. annuum fruit. 63 High temperature and light irradiation activated carotenoid biosynthesis genes such as PSY, CRTZ, ζ-Carotene desaturase (ZDS), and CCS in green pepper fruit and induced the carotenoid formation in C. annuum (Figure 2). 130 Low temperature and pre-and postharvest application of low doses of UVB and C elicited chlorogenic acid, apigenin-7-Oglucoside, and luteolin-7-O-glucoside (flavonoids) and carotenoid accumulation in pepper fruits and leaves.…”

Section: Elicitor Treatment and Mineralmentioning

confidence: 99%

Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

Islam

Rawoof

Kumar

et al. 2023

J. Agric. Food Chem.

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Capsicum (chili pepper) is a widely popular and highly consumed fruit crop with beneficial secondary metabolites such as capsaicinoids, carotenoids, flavonoids, and polyphenols, among others. Interestingly, the secondary metabolite profile is a dynamic function of biosynthetic enzymes, regulatory transcription factors, developmental stage, abiotic and biotic environment, and extraction methods. We propose active manipulable genetic, environmental, and extraction controls for the modulation of quality and quantity of desired secondary metabolites in Capsicum species. Specific biosynthetic genes such as Pun (AT3) and AMT in the capsaicinoids pathway and PSY, LCY, and CCS in the carotenoid pathway can be genetically engineered for enhanced production of capsaicinoids and carotenoids, respectively. Generally, secondary metabolites increase with the ripening of the fruit; however, transcriptional regulators such as MYB, bHLH, and ERF control the extent of accumulation in specific tissues. The precise tuning of biotic and abiotic factors such as light, temperature, and chemical elicitors can maximize the accumulation and retention of secondary metabolites in pre-and postharvest settings. Finally, optimized extraction methods such as ultrasonication and supercritical fluid method can lead to a higher yield of secondary metabolites. Together, the integrated understanding of the genetic regulation of biosynthesis, elicitation treatments, and optimization of extraction methods can maximize the industrial production of secondary metabolites in Capsicum.

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“…In addition, studies have shown that overexpressing AtHDG11 in wild-type Arabidopsis and tobacco have altered stomatal density and root development, as well as increased ABA content and improved photosynthesis, resulting in increased drought tolerance and biomass ( Yu et al., 2008 ). Other studies have also shown that AtHDG11 improves drought tolerance in sweetpotato, tall fescue, rice, cotton and poplar due to a series of beneficial changes at the morphological and physiological level of transgenic plants, such as developed root systems and reduced stomatal density ( Cao et al., 2009 ; Ruan et al., 2012 ; Yu et al., 2013 ; Yu et al., 2016 ).…”

Section: Hd-zip Proteins Regulate Drought Tolerance By Controlling Pl...mentioning

confidence: 99%

The roles of HD-ZIP proteins in plant abiotic stress tolerance

Yang

Zhang

et al. 2022

Front. Plant Sci.

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Homeodomain leucine zipper (HD-ZIP) proteins are plant-specific transcription factors that contain a homeodomain (HD) and a leucine zipper (LZ) domain. The highly conserved HD binds specifically to DNA and the LZ mediates homodimer or heterodimer formation. HD-ZIP transcription factors control plant growth, development, and responses to abiotic stress by regulating downstream target genes and hormone regulatory pathways. HD-ZIP proteins are divided into four subclasses (I–IV) according to their sequence conservation and function. The genome-wide identification and expression profile analysis of HD-ZIP proteins in model plants such as Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) have improved our understanding of the functions of the different subclasses. In this review, we mainly summarize and discuss the roles of HD-ZIP proteins in plant response to abiotic stresses such as drought, salinity, low temperature, and harmful metals. HD-ZIP proteins mainly mediate plant stress tolerance by regulating the expression of downstream stress-related genes through abscisic acid (ABA) mediated signaling pathways, and also by regulating plant growth and development. This review provides a basis for understanding the roles of HD-ZIP proteins and potential targets for breeding abiotic stress tolerance in plants.

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A novel gene, CaATHB-12, negatively regulates fruit carotenoid content under cold stress in Capsicum annuum

Cited by 13 publications

References 99 publications

Characterization of Phytochrome-Interacting Factor Genes in Pepper and Functional Analysis of CaPIF8 in Cold and Salt Stress

Characterization of Phytochrome-Interacting Factor Genes in Pepper and Functional Analysis of CaPIF8 in Cold and Salt Stress

Genetic Regulation, Environmental Cues, and Extraction Methods for Higher Yield of Secondary Metabolites in Capsicum

The roles of HD-ZIP proteins in plant abiotic stress tolerance

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