A novel fluorescence biosensor based on double-stranded DNA branch migration-induced HCR and DNAzyme feedback circuit for sensitive detection of Pseudomonas aeruginosa (clean version)

Cascade branch migration-triggered strand displacement amplification for specific and sensitive detection of microRNA

“…21 In the previous research, we utilized the double-stranded DNA branch migration to produce two Y-shaped probes, which initiated a DNAzyme circuit and hybridization chain reaction (HCR) to achieve effective signal amplification. 22 Considering that the binding of one Y-shaped probe and a ssDNA strand can easily form a 3WJ structure, the 3WJ-triggered SDA may be an ideal amplification method for cascading the CBM process. A G-quadruplex (G4) is a short DNA fragment formed by the folding of G-rich ssDNA in the presence of specific cations, especially potassium ions.…”

Hook-Like DNAzyme-Activated Autocatalytic Biosensor for the Universal Detection of Pathogenic Bacteria

“…Although the integration of DNAzyme and enzyme-free signal amplification has been explored for bacterial detection, 8,18,31 Inspired by the versatility of a tractor hook, which adapts to various attachments, we introduced a hook element to seamlessly transmit bacterial recognition signals from different DNAzymes to the signal amplification process. In this study, we developed a hook-like DNAzyme-activated autocatalytic hybridization assembly (DAHA) circuit for universal bacterial detection.…”

“…Functional nucleic acids (DNAzyme and aptamer) have emerged as rapid, cost-effective, and robust alternatives for bacterial detection. − In particular, RNA-cleaving fluorogenic DNAzyme serves as a potent tool for bacterial recognition, catalyzing the cleavage of chimeric RNA/DNA substrates and producing fluorescence signals upon the presence of target bacteria. , DNAzyme-based biosensors have been developed for the detection of various bacteria. − Nonetheless, a significant limitation of DNAzyme is the low enzymatic activity, which necessitates the employment of signal amplification strategies to achieve enhanced detection sensitivity …”

“…Although the integration of DNAzyme and enzyme-free signal amplification has been explored for bacterial detection, ,, it presents notable challenges in developing a universal detection platform. These methods entail the incorporation of DNAzyme-recognized molecular signals into signal amplification processes, either by establishing a novel amplification circuit specific to the DNAzyme system or by devising connecting modules between the DNAzyme and an existing circuit.…”