A novel epigenetic regulation of circFoxp1 on Foxp1 in colon cancer cells

Luo, Yanwei; Liu, Fengxia; Ma, Jinqi; Fu, Yunfeng; Gui, Rong

doi:10.1038/s41419-020-03007-6

Cited by 12 publications

(5 citation statements)

References 20 publications

(28 reference statements)

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“…In colon cancer tissues, the expression of circFOXP1 and FOXP1 was negatively correlated. CircFOXP1 recruits DNMT1 to hypermethylated the promoter of FOXP1, thereby inhibiting the expression of FOXP1, and ultimately facilitating tumor progression in colon cancer 28 . In our previous study, we identified that circFOXP1 expression was remarkably upregulated in gallbladder cancer tissues and cells, and promoted tumor progression and the Warburg effect in GBC cells by regulating PKLR expression 12 .…”

Section: Discussionmentioning

confidence: 99%

ALKBH5-mediated m6A modification of circFOXP1 promotes gastric cancer progression by regulating SOX4 expression and sponging miR-338-3p

Wang,

Zhu,

Hao

et al. 2024

Commun Biol

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Circular RNAs (circRNAs) have recently been suggested as potential functional modulators of cellular physiology processes in gastric cancer (GC). In this study, we demonstrated that circFOXP1 was more highly expressed in GC tissues. High circFOXP1 expression was positively associated with tumor size, lymph node metastasis, TNM stage, and poor prognosis in patients with GC. Cox multivariate analysis revealed that higher circFOXP1 expression was an independent risk factor for disease-free survival (DFS) and overall survival (OS) in GC patients. Functional studies showed that increased circFOXP1 expression promoted cell proliferation, cell invasion, and cell cycle progression in GC in vitro. In vivo, the knockdown of circFOXP1 inhibited tumor growth. Mechanistically, we observed ALKBH5-mediated m6A modification of circFOXP1 and circFOXP1 promoted GC progression by regulating SOX4 expression and sponging miR-338-3p in GC cells. Thus, our findings highlight that circFOXP1 could serve as a novel diagnostic and prognostic biomarker and potential therapeutic target for GC.

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Section: Discussionmentioning

confidence: 99%

ALKBH5-mediated m6A modification of circFOXP1 promotes gastric cancer progression by regulating SOX4 expression and sponging miR-338-3p

Wang,

Zhu,

Hao

et al. 2024

Commun Biol

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“…Moreover, it was shown that downregulation of the miR-34 family in OC is associated with more aggressive disease . FOXP1, a transcription factor, a known target of miR-34a, is identified as an oncogene that upregulated in several malignancies. − Upregulation of FOXP1 correlated with chemoresistance in FIGO stage III serous OC and high cytoplasmic FOXP1 expression in EOC was associated with a higher tumor grade …”

Section: Discussionmentioning

confidence: 99%

miR-34a-FOXP1 Loop in Ovarian Cancer

Dirimtekin,

Mortoglou,

Alavanda

et al. 2023

ACS Omega

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Ovarian cancer (OC) is the main cause of gynecological cancer mortality in most developed countries. microRNA (miR) expression dysregulation has been highlighted in human cancers, and miR-34a is found to be downregulated and associated with inhibition of tumor growth and invasion in several malignancies, including OC. The winged helix transcription factor forkhead box P1 (FOXP1) is reported as either an oncogene or tumor suppressor in various cancers. This study aimed to elucidate potential clinical and biological associations of miR-34a and transcription factor FOXP1 in OC. We investigated nine OC patients’ blood samples and two OC cell lines (SKOV-3 and OVCAR-3) using quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) to determine both miR-34a and FOXP1 expressions. We have found that miR-34a and FOXP1 are reversely correlated in both in vitro and in vivo. Inhibition of miR-34a transiently led to upregulation of FOXP1 mRNA expression and increased cellular invasion in vitro. Our data indicate that miR-34a could be a potential biomarker for improving the diagnostic efficiency of OC, and miR-34a overexpression may reduce OC pathogenesis by targeting FOXP1.

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“…CCK-8 assay was performed as described in our previous described ( 27 ). A CCK-8 kit was used for determining the cell viability of NCCIT and Tcam-2 cells after siRNA transfection according to the instructions.…”

Section: Methodsmentioning

confidence: 99%

Long Non-Coding RNA RFPL3S Functions as a Biomarker of Prognostic and Immunotherapeutic Prediction in Testicular Germ Cell Tumor

et al. 2022

Self Cite

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The incidence of testicular germ cell tumor (TGCT) is currently on the rise worldwide, of which 15%-30% of patients have occur recurrence and metastasis. However, clinical methods for diagnosing TGCT and judging its prognosis remained inadequate. In this study, we aimed to explore the possibility of testis-specific long-chain non-coding RNA (lncRNA) Ret finger protein-like 3S (RFPL3S) as a biomarker for TGCT diagnosis, prognosis, and treatment response by reviewing the TGCT gene expression data in Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases. The cohort data and DNA methylation data of TGCT in TCGA were downloaded from TGCA, UCSC XENA, and GEO. The bioinformatic tools were used, including GEPIA2, Kaplan-Meier Plotter, LinkedOmics, UCSC XENA, Sangerbox Tools, GSCA, and Tumor Immune Dysfunction and Exclusion. Compared with normal testicular tissues, the RFPL3S expression was significantly reduced in TGCT, and was significantly negatively correlated with the patient’s Tumor, Node, Metastasis stage. Hypermethylation and low copy number of RFPL3S were present in TGCT, and low RFPL3S was associated with short disease-free and progression-free intervals. Silencing RFPL3S significantly enhanced the invasion ability and proliferation ability of TGCT cells as evaluated by Transwell and CCK-8 experiments. Additionally, RFPL3S expression was positively correlated with the infiltration of immune-activating cells such as B cells, CD8+ T cells, cytotoxic T cells, and natural killer cells, and negatively correlated with the infiltration of immunosuppressive cells such as Th17 and Th2. Higher RFPL3S expression was present in patients with immunotherapy benefits. In conclusion, we determined that the testis-specific lncRNA RFPL3S functioned as a tumor suppressor in TGCT and could be used as a prognostic predictor of TGCT, as well as a marker to predict the effect of TGCT immunotherapy.

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A novel epigenetic regulation of circFoxp1 on Foxp1 in colon cancer cells

Cited by 12 publications

References 20 publications

ALKBH5-mediated m6A modification of circFOXP1 promotes gastric cancer progression by regulating SOX4 expression and sponging miR-338-3p

ALKBH5-mediated m6A modification of circFOXP1 promotes gastric cancer progression by regulating SOX4 expression and sponging miR-338-3p

miR-34a-FOXP1 Loop in Ovarian Cancer

Long Non-Coding RNA RFPL3S Functions as a Biomarker of Prognostic and Immunotherapeutic Prediction in Testicular Germ Cell Tumor

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