A novel Ehrlichia genotype strain distinguished by the TRP36 gene naturally infects cattle in Brazil and causes clinical manifestations associated with ehrlichiosis

Aguiar, Daniel Moura de; Ziliani, Thayza F.; Zhang, Xiaofeng; Melo, Andréia Lima Tomé; Braga, Ísis Assis; Witter, Rute; Freitas, Leodil C.; Rondelli, André Luiz Hoeppner; Luis, Michele A.; Sorte, Eveline C.B.; Jaune, Felipe Wolf; Santarém, Vamilton Álvares; Horta, Maurício Cláudio; Pescador, Carolina Argenta; Colodel, Edson Moleta; Soares, Herbert Sousa; Pacheco, Richard C.; Onuma, Selma Samiko Miyazaki; Labruna, Marcelo B.; McBride, Jere W.

doi:10.1016/j.ttbdis.2014.03.010

Cited by 68 publications

(79 citation statements)

References 28 publications

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“…The existence of cross-reactivity observed in the IFA among the species of the genus Ehrlichia (HARRUS & WANER, 2011) implies the possibility that an unrecognized ehrlichial agent is circulating among these animals. To date, only E. canis and an ehrlichial agent related to cattle and Rhipicephalus (Boophilus) microplus ticks have been isolated in Brazil (CABEZAS-CRUZ et al, 2012;AGUIAR et al, 2013AGUIAR et al, , 2014.…”

Section: Discussionmentioning

confidence: 99%

Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of São Paulo, Brazil

Melo

Aguiar

Spolidorio

et al. 2016

Rev. Bras. Parasitol. Vet.

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Evidência sorológica de exposição a agentes transmitidos por carrapatos em gambás (Didelphis spp.) AbstractThis work involved a serological investigation of tick-borne pathogens in opossums in eight municipalities of the state of São Paulo, Brazil. Serum samples from 109 opossums (91 Didelphis aurita and 18 Didelphis albiventris) were tested to detect antibodies to Rickettsia rickettsii (Taiaçu strain, 1:64 cut-off) and Ehrlichia canis (São Paulo strain, 1:40 cut-off), by indirect immunofluorescence assay (IFA); and against Borrelia burgdorferi (strain G39/40) by enzyme-linked immunosorbent assay (ELISA). The presence of antibodies to anti-R. rickettsii, anti-E. canis and anti-B. burgdorferi was detected in 32 (29.35%), 16 (14.67%) and 30 (27.52%) opossums, respectively. Opossum endpoint titers ranged from 64 to 1,024 for R. rickettsii, from 40 to 160 for E. canis, and from 400 to >51,200 for B. burgdorferi. These serological results suggest that opossums have been exposed to Rickettsia spp., Ehrlichia spp., and B. burgdorferi-related agents in the state of São Paulo. Our study underscores the need for further research about these agents in this study area, in view of the occurrence of Spotted Fever and Baggio-Yoshinari Syndrome disease in humans in the state of São Paulo, Brazil.

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Section: Discussionmentioning

confidence: 99%

Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of São Paulo, Brazil

Melo

Aguiar

Spolidorio

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…UFMG-EV and Ehrlichia sp. UFMT-BV, however, present new sequence of tandem repeats different to the one reported for E. canis TRP36 [2,5,6].…”

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confidence: 60%

“…Secondly, while E. canis is mainly pathogenic for dog [10], Ehrlichia sp. UFMT-BV was found to be pathogenic for cattle [5]. How pathogens can colonize new hosts is a challenging question in evolutionary biology [13].…”

Section: Model Of Emergence Of Ehrlichia Sp Ufmg-ev and Ehrlichia Spmentioning

confidence: 99%

“…UFMG-EV (referred as E. mineirensis in [2,3]) that was isolated from R. microplus hemolymph [2][3][4], and (iii) Ehrlichia sp. UFMT-BV that was found to be pathogenic for cattle in Brazil [5]. These three organisms are closely related to E. canis [1,2,5].…”

mentioning

confidence: 99%

“…UFMT-BV that was found to be pathogenic for cattle in Brazil [5]. These three organisms are closely related to E. canis [1,2,5]. Ehrlichia sp.…”

mentioning

confidence: 99%

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The glycoprotein TRP36 of Ehrlichia sp. UFMG-EV and related cattle pathogen Ehrlichia sp. UFMT-BV evolved from a highly variable clade of E. canis under adaptive diversifying selection

Cabezas‐Cruz

Valdés

Fuente

2014

Parasites & Vectors

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Background: A new species of Ehrlichia, phylogenetically distant from E. ruminantium, was found in 2010 infecting cattle in Canada. In 2012 and 2013, we reported the in vitro propagation, molecular and ultrastructural characterization of Ehrlichia sp. UFMG-EV (E. mineirensis), a new species of Ehrlichia isolated from the haemolymph of Brazilian Rhipicephalus (Boophilus) microplus ticks. A new organism, named Ehrlichia sp. UFMT-BV, closely related to Ehrlichia sp. UFMG-EV, was recently described in Brazil and after experimental infection it was shown to be pathogenic for cattle. This new emerging clade of cattle Ehrlichia pathogens is closely related to E. canis. The major immunogenic Tandem Repeat Protein (TRP36; also known as gp36) is extensively used to characterize the genetic diversity of E. canis. Homologs of TRP36 were found in both Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV. Findings: Herein, we characterized the evolution of this new Ehrlichia clade using TRP36 sequences. Our working hypothesis is that Ehrlichia sp. UFMG-EV and related microorganisms evolved from a highly variable E. canis clade. In support of our hypothesis we found that Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV TRP36 evolved from a highly divergent and variable clade within E. canis and this clade evolved under episodic diversifying selection with a high proportion of sites under positive selection. Conclusion: Our results suggest that Ehrlichia sp. UFMG-EV and Ehrlichia sp. UFMT-BV evolved from a variable clade within E. canis.

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Rickettsiales

McBride¹,

Ganta²,

Walker³

2022

Pathogenesis of Bacterial Infections in Animals

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A novel Ehrlichia genotype strain distinguished by the TRP36 gene naturally infects cattle in Brazil and causes clinical manifestations associated with ehrlichiosis

Cited by 68 publications

References 28 publications

Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of São Paulo, Brazil

Serological evidence of exposure to tick-borne agents in opossums (Didelphis spp.) in the state of São Paulo, Brazil

The glycoprotein TRP36 of Ehrlichia sp. UFMG-EV and related cattle pathogen Ehrlichia sp. UFMT-BV evolved from a highly variable clade of E. canis under adaptive diversifying selection

Rickettsiales

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