A Novel Double‐Network Hydrogel Induces Spontaneous Articular Cartilage Regeneration <i>in vivo</i> in a Large Osteochondral Defect

Yasuda, Kazunori; Kitamura, Nobuto; Gong, Jian Ping; Arakaki, Keiichi; Kwon, Hyuck Joon; Onodera, Shin; Chen, Yong Mei; Kurokawa, Takayuki; Kanaya, Fuminori; Ohmiya, Yoshihiro; Osada, Yoshihito

doi:10.1002/mabi.200800223

Cited by 163 publications

(164 citation statements)

References 38 publications

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“…Despite their high water content (90 wt%), DN gels synthesized with such an optimized composition exhibit high elastic modulus (0.3-1.0 MPa), and high compressive fracture strengths, similar to natural cartilages (20 MPa) [11,13]. The DN principle to synthesize the tough hydrogels was further applied to various polymer combinations, and tough DN gels with good biocompatibility [14][15][16], low sliding friction [17], and excellent wear resistance [18] have been synthesized.…”

Section: Introductionmentioning

confidence: 99%

Formation of a strong hydrogel–porous solid interface via the double-network principle

et al. 2010

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Section: Introductionmentioning

confidence: 99%

Formation of a strong hydrogel–porous solid interface via the double-network principle

et al. 2010

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“…The overall charge carried on hydrogels can heavily influence cell adhesion and behavior. Positively charged surfaces are known to increase cell attachment and proliferation; as demonstrated by Schneider et al [23] Negatively charged hydrogels have also been shown to increase proliferation of endothelial cells [30] and improve cartilage regeneration in vivo, [31] although Liu et al [32] observed decreased fibroblast proliferation on negatively charged hydrogels. These differences in findings about which charge cells favor lead us to speculate that certain cells prefer a specific charge, be it positive or negative.…”

Section: Discussionmentioning

confidence: 99%

Peptide Hydrogels—A Tissue Engineering Strategy for the Prevention of Oesophageal Strictures

Kumar

Workman

O’Brien

et al. 2017

Adv Funct Materials

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Endoscopic treatment of Barrett's oesophagus often leads to further damage of healthy tissue causing fibrotic tissue formation termed as strictures. This study shows that synthetic, self-assembling peptide hydrogels (PeptiGelDesign) support the activity and function of primary oesophageal cells, leading to epithelialization and stratification during in vitro 3D co-culture. Following buffering in culture media, rat oesophageal stromal fibroblasts (rOSFs) are incorporated into a library of peptide hydrogels, whereas mouse oesophageal epithelial cells (mOECs) are seeded on the surface. Optimal hydrogels (PGD-AlphaProC and PGD-CGD2) support mOEC viability (>95%), typical cell morphology (cobblestone-like), and slower migration over a shorter distance compared to a collagen control, at 48 h. Positive expression of typical epithelial markers (ZO-1 and cytokeratins) is detected using immunocytochemistry at day 3 in culture. Furthermore, optimal hydrogels are identified which support rOSF viability (>95%) with homogeneous distribution when incorporated into the hydrogels and also promote the secretion of collagen type I detected using an enzyme linked immunosorbent assay (ELISA), at day 7. A 3D co-culture model using optimal hydrogels for both cell types supports a stratified epithelial layer (expressing involucrin and AE1/AE3 markers). Findings from this study could lead to the use of peptide hydrogels as a minimally invasive endoscopic therapy to manage oesophageal strictures.

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“…NaSS and NaAMPS have good biocompatibility, which means nontoxic due to their sodium-form of chemical structure. Indeed, DN gels are used as test beds for cell culture [6]. DMAAm is convenient to be used due to its high reactivity in gelation and good ductility.…”

Section: Introductionmentioning

confidence: 99%

Novel Optical Devices Developed with High-Strength Gels

Yokoo

Hidema

Furukawa

2011

JMMP

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Double network (DN) gels are anomalously robust but soft and wet materials, whose maximum stress in compression reaches 30MPa and surface friction coefficient reaches 0.001 simultaneously. The DN gels also have shock absorbency, permeability, and biocompatibility. Thus it is expected that the DN gels will be applied to artificial joints, artificial cartilages, and artificial blood vessels. In this study we focused on both high transparency and large transformation of the DN gels, and we tried to develop novel optical devices. Our purpose is to apply it to a deformable lens system. Indeed, we succeed to make an artificial gel lens, which is nontoxic and have biocompatibility. The value of strain of the gel lens can achieve more than 2, and Young's moduli of our two kinds of DN gels show 0.45MPa and 0.46 10 -1 MPa, which is enough higher than human lens. We will realize further miniaturization and simplification of various optical products containing the gel lens.

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A Novel Double‐Network Hydrogel Induces Spontaneous Articular Cartilage Regeneration in vivo in a Large Osteochondral Defect

Cited by 163 publications

References 38 publications

Formation of a strong hydrogel–porous solid interface via the double-network principle

Formation of a strong hydrogel–porous solid interface via the double-network principle

Peptide Hydrogels—A Tissue Engineering Strategy for the Prevention of Oesophageal Strictures

Novel Optical Devices Developed with High-Strength Gels

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