A novel cysteine proteinase (CP65) of Trichomonas vaginalis involved in cytotoxicity

Álvarez-Sánchez, María Elizbeth; Ávila‐González, Leticia; Becerril-Garcia, C.; Fattel-Facenda, L. V.; Ortega‐López, Jaime; Arroyo, Rossana

doi:10.1006/mpat.1999.0336

Cited by 105 publications

(129 citation statements)

References 30 publications

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“…Some authors have claimed that T. vaginalis exhibited an increased ratio of attachment and cytotoxicity on epithelial cells compared to fibroblasts (Alderete & Garza 1985). However, these data were not confirmed and other studies have shown that trichomonads are capable of attaching to substrates such as laminin, fibronectin and collagen, which are known components of the extracellular matrix and basement membrane (Costa e Silva-Filho et al 1988, Benchimol et al 1990, Crouch & Alderete 1999, Alvarez-Sánchez et al 2000.…”

Section: Discussionmentioning

confidence: 93%

Interaction of Trichomonas vaginalis and Tritrichomonas foetus with keratin: an important role in parasite infection

Vilela

Benchimol

2011

Mem. Inst. Oswaldo Cruz

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Trichomonas vaginalis and Tritrichomonas foetus are human and bovine parasites, respectively, that provoke the sexually transmitted disease trichomoniasis. These extracellular parasites adhere to the host epithelial cell surface. Although mucinases and proteases have been described as important proteins for parasite adhesion to epithelial cells, no studies have examined the role of the keratin molecules that cornify the vaginal epithelium. Here, we investigated the interaction of T. vaginalis and T. foetus with human keratin in vitro; additionally, adherence assays were performed in cattle with T. foetus to elucidate whether trichomonads were able to interact with keratin in vivo. We demonstrated that both T. vaginalisand T. foetusinteracted directly with keratin. Additionally, the trichomonads ingested and digested keratin, shedding new light on the Trichomonas infection process

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Section: Discussionmentioning

confidence: 93%

Interaction of Trichomonas vaginalis and Tritrichomonas foetus with keratin: an important role in parasite infection

Vilela

Benchimol

2011

Mem. Inst. Oswaldo Cruz

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“…At least 23 and 15 different cysteine proteases have been identified in T. vaginalis and bovine T. foetus , respectively 60, 67, 68, 69, 70. At least three T. vaginalis surface‐expressed cysteine proteases (CP30, CP65 and TvLEGU‐1) play a key role in adhesion and adhesion‐dependent cytotoxicity to cervical and vaginal epithelial cells 48, 66, 71, 72, 73, 74…”

Section: Pathogenesis Of Trichomonosismentioning

confidence: 99%

“…Many of the T. vaginalis cysteine proteases have been identified in vaginal secretions from infected women 84, 85. The cytotoxic effects of cysteine proteases include degradation of mucus, complement and immunoglobulin, and destruction of extracellular matrix (fibronectin, several types of collagen, and laminin) 43, 72, 73, 84, 86, 87. Cysteine proteases therefore can function as epithelial cell detaching factors and can mediate direct cytotoxic effects on vaginal and uterine epithelial cells by induction of apoptosis72, 73, 84, 88, 89, 90 (Fig 1D).…”

Section: Pathogenesis Of Trichomonosismentioning

confidence: 99%

Mechanisms ofTritrichomonas foetusPathogenicity in Cats with Insights from Venereal Trichomonosis

Tolbert

Gookin

2016

Veterinary Internal Medicne

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Almost 20 years has passed since trichomonosis was first recognized as a potential cause of diarrhea in domestic cats. Despite progress in confirming disease causation, developing means for diagnosis, and identifying approaches to treatment of the infection, we still know very little about how this parasite causes diarrhea. With increasing recognition of resistance of trichomonosis to treatment with 5‐nitroimidazole drugs, new treatment strategies based on an understanding of disease pathogenesis are needed. In this review, lessons learned from the pathogenesis of venereal trichomonosis in people and cattle are applied to clinical observations of trichomonosis in cats in effort to generate insight into areas where further research may be beneficial.

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“…Cyto-adherence and cytotoxicity (Petrópolis et al 2008;Ramon-Luing Lde et al 2011) are thought to be the principal mechanism. Binding of trichomonads to vaginal epithelial cells for colonization and infection is dependent on adhesion molecules on the surface of the parasites, either alone or with other soluble parasite molecules such as hydrolytic enzymes (Peterson and Alderete 1982;Neale and Alderete 1990;Lockwood et al 1984;Hernandez et al 2011), cytotoxic molecules (Ramon-Luing Lde et al 2011;Lucas et al 2008), cell detaching (Garber et al 1989), or cell lytic factors (Alvarez-Sanchez et al 2000;Kennett et al 2002).…”

Section: Pathogenesismentioning

confidence: 99%

Comparative aspects of immunity and vaccination in human and bovine trichomoniasis: a review

Chapwanya

Usman

Irons

2015

Trop Anim Health Prod

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Trichomonas vaginalis and Tritrichomonas foetus are important extracellular protozoans that cause, respect i v e l y, h u m a n a n d b o v i n e v e n e r e a l d i s e a s e s . Trichomonads are extracellular parasites that primarily inhabit the genital tracts of the mammalian hosts where they overcome the mucus barrier and parasitize mucosa by contact-dependent or contact-independent cytotoxicity. Transient immunity is usually achieved by the host after clinical infection. At present, vaccination in cattle reduces infection rates and reproductive wastage in affected herds. After vaccination, immunoglobulin G (IgG) levels increase in systemic circulation while immunoglobulin A (IgA) levels rise in the vagina. Only moderate protection is conferred by means of vaccination. Future vaccine development strategies are needed for cattle to enhance the antigenic component or use adjuvant that strongly activates the innate immune response to produce safe and potent vaccines. This paper reviews the current knowledge of the immunology of trichomoniasis infection and the challenges and potential of vaccines in the control of the infection in human and bovine trichomoniasis.

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A novel cysteine proteinase (CP65) of Trichomonas vaginalis involved in cytotoxicity

Cited by 105 publications

References 30 publications

Interaction of Trichomonas vaginalis and Tritrichomonas foetus with keratin: an important role in parasite infection

Interaction of Trichomonas vaginalis and Tritrichomonas foetus with keratin: an important role in parasite infection

Mechanisms ofTritrichomonas foetusPathogenicity in Cats with Insights from Venereal Trichomonosis

Comparative aspects of immunity and vaccination in human and bovine trichomoniasis: a review

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