A Novel Cell Disruption Approach: Effectiveness of Laser-induced Cell Lysis of Pichia pastoris in the Continuous System

Gazor, Maryam; Talesh, S. Siamak Ashraf; Kavianpour, Alireza; Khatami, Maryam; Javidanbardan, Amin; Hosseini, Seyed Nezamedin

doi:10.1007/s12257-017-0261-6

Cited by 17 publications

(14 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analytical grade chemicals, purchased from Merck Company (Darmstadt, Germany), were used to prepare culture media and buffers. The Mut + strain of P. pastoris GS115 (His4) expressing intracellular HBsAg under the control of the AOX1 promoter was used for commercial production purposes . For the ANN program, Matlab toolbox from Matlab R2016b (ver.…”

Section: Methodsmentioning

confidence: 99%

Accurate and cost‐effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pastoris by using neural network based soft sensor

Hosseini

Javidanbardan

Khatami

2019

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

In the current work, the attempt was made to apply best-fitted artificial neural network (ANN) architecture and the respective training process for predicting final titer of hepatitis B surface antigen (HBsAg), produced intracellularly by recombinant Pichia pastoris Mut + in the commercial scale. For this purpose, in large-scale fed-batch fermentation, using methanol for HBsAg induction and cell growth, three parameters of average specific growth rate, biomass yield, and dry biomass concentration-in the definite integral form with respect to fermentation time-were selected as input vectors; the final concentration of HBsAg was selected for the ANN output. Used dataset consists of 38 runs from previous batches; feed-forward ANN 3:5:1 with training algorithm of backpropagation based on a Bayesian regularization was trained and tested with a high degree of accuracy. Implementing the verified ANN for predicting the HBsAg titer of the five new fermentation runs, excluded from the dataset, in the full-scale production, the coefficient of regression and root-mean-square error were found to be 0.969299 and 2.716774, respectively. These results suggest that this verified soft sensor could be an excellent alternative for the current relatively expensive and time-intensive analytical techniques such as enzyme-linked immunosorbent assay in the biopharmaceutical industry.

show abstract

Section: Methodsmentioning

confidence: 99%

Accurate and cost‐effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pastoris by using neural network based soft sensor

Hosseini

Javidanbardan

Khatami

2019

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

show abstract

“…Yeast cells have a relatively more rigid cell wall structure than prokaryotes like bacteria; thus, cell disruption technique must be carefully evaluated to release all the desired intracellular recombinant products . Different studies have evaluated the advantages and disadvantages of various well‐established cell disruption methods for bioanalytical and industrial purposes . Non‐mechanical cell lysis techniques including enzymatic and chemical ones are mild and selective methods that are mostly applied in laboratory‐scale for bioanalytical purposes .…”

Section: Introductionmentioning

confidence: 99%

“…Different studies have evaluated the advantages and disadvantages of various well‐established cell disruption methods for bioanalytical and industrial purposes . Non‐mechanical cell lysis techniques including enzymatic and chemical ones are mild and selective methods that are mostly applied in laboratory‐scale for bioanalytical purposes . The major drawback of these methods is their inapplicability in large scale .…”

Section: Introductionmentioning

confidence: 99%

“…The major drawback of these methods is their inapplicability in large scale . Physical techniques including ultrasonic and thermolysis are another category of non‐mechanical techniques, which can be employed in both laboratory‐scale and large‐scale processes . Nonetheless, these methods are rarely applied in the production process of recombinant proteins, specifically therapeutic ones since these methods could adversely affect the stability and potency of the protein of interest .…”

Section: Introductionmentioning

confidence: 99%

“…The mechanical methods including bead mill and high‐pressure homogenizer are based on solid shears and liquid shears, respectively, and they are widely used in commercial scale . The robustness of these techniques on cell disruption is well proven; however, since these techniques are non‐specific in cell disruption, they make the subsequent clarification and purification processes more challenging . Possible denaturation due to shear stress, high capital investment, and energy dissipation are reported as other drawbacks of utilizing these techniques in cell disruption .…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

High recovery of intracellular recombinant HBsAg from Pichia pastoris via continuous pulsed laser cell disruption system optimized by response surface methodology

Gazor

Talesh

Hosseini

et al. 2018

Biotech and App Biochem

Self Cite

View full text Add to dashboard Cite

In our previous study, we demonstrated that continuous power laser could be a clean, rapid, and convenient alternative to the other conventional disruption techniques for the release of recombinant hepatitis B surface antigen (rHBsAg) from Pichia pastoris. In the current work, we examined the effect of pulsed laser in the continuous laboratory-scale process on cell disruption. Design-of-experiments (DOE) methodology was used for optimization of cell disruption process to obtain the highest protein concentration in the disruption buffer. Our investigations for the pulsed laser at wavelength of 1,064 nm demonstrated that for disrupting P. pastoris cell and releasing rHBsAg, the laser power was the most influential factor, and laser pulse duration and cycle number were in the second and third places. According to the results, the effect of laser power and pulse duration (time) had a direct relationship with protein concentration. For the number of cycles, however, increasing the value from the lowest point at first led to the enhancement and then reduction of protein concentration. The maximum cell disruption and rHBsAg release were recorded for the laser system in the energy input of 284 mW and the pulse duration of 100 mSec after four complete rounds of circulation. C 2018

show abstract

New horizons in developing cell lysis methods: A review

Danaeifar

2022

Biotech & Bioengineering

View full text Add to dashboard Cite

Cell lysis is an essential step in many studies related to biology and medicine. Based on the scale and medium that cell lysis is carried out, there are three main types of the cell lysis: (1) lysis of the cells in the surrounding environment, (2) lysis of the isolated or cultured cells, and (3) single cell lysis. Conventionally, several cell lysis methods have been developed, such as freeze‐thawing, bead beating, incursion in liquid nitrogen, sonication, and enzymatic and chemical‐based approaches. In recent years, various novel technologies have been employed to develop new methods of cell lysis. The aim of studies in this field is to introduce more precise and efficient tools or to reduce the costs of cell lysis procedures. Nanostructure‐based lysis methods, acoustic oscillation, electrical current, irradiation, bacteria‐mediated cell lysis, magnetic ionic liquids, bacteriophage genes, monolith columns, hydraulic forces, and steam explosion are some examples of newly developed cell lysis methods. Besides the significant advances in this field, there are still many challenges and tools must be further improved.

show abstract

A Novel Cell Disruption Approach: Effectiveness of Laser-induced Cell Lysis of Pichia pastoris in the Continuous System

Cited by 17 publications

References 22 publications

Accurate and cost‐effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pastoris by using neural network based soft sensor

Accurate and cost‐effective prediction of HBsAg titer in industrial scale fermentation process of recombinant Pichia pastoris by using neural network based soft sensor

High recovery of intracellular recombinant HBsAg from Pichia pastoris via continuous pulsed laser cell disruption system optimized by response surface methodology

New horizons in developing cell lysis methods: A review

Contact Info

Product

Resources

About