A novel biosensor array with a wheel-like pattern for glucose, lactate and choline based on electrochemiluminescence imaging

Abstract

Electrochemiluminescence (ECL) imaging provides a superior approach to achieve array detection because of its ability for ultrasensitive multiplex analysis. In this paper, we reported a novel ECL imaging biosensor array modified with an enzyme/carbon nanotubes/chitosan composite film for the determination of glucose, choline and lactate. The biosensor array was constructed by integrating a patterned indium tin oxide (ITO) glass plate with six perforated poly(dimethylsiloxane) (PDMS) covers. ECL is generated by the electrochemical reaction between luminol and hydrogen peroxide that is produced by the enzyme catalysed oxidation of different substrates with molecular oxygen, and ECL images were captured by a charge-coupled device (CCD) camera. The separated electrochemical micro-cells enabled simultaneous assay of six samples at different concentrations. From the established calibration curves, the detection limits were 14 μM for glucose, 40 μM for lactate and 97 μM for choline, respectively. Moreover, multicomponent assays and cross reactivity were also studied, both of which were satisfied for the analysis. This biosensing platform based on ECL imaging shows many distinct advantages, including miniaturization, low cost, and multi-functionalization. We believe that this novel ECL imaging biosensor platform will have potential applications in clinical diagnostics, medicine and food inspection.

Electrochemiluminescence DNA sensor array for multiplex detection of biowarfare agents

“…Novel ECL labels, including quantum dots with different emission wavelengths, have been used for multiplex ECL protein assays [14,15]. Multiplex enzyme assays for the multiplex imaging of glucose, choline, and lactate on carbon nanotubes functionalised with chitosan and specific enzymes have been reported [16]. Six individually addressable areas were created with a PDMS mask, and a CCD camera was used to detect the ECL signal generated upon interaction of luminol with hydrogen peroxide formed via the enzymatic reactions.…”

Parallel Detection of Refractive Index Changes in a Porous Silicon Microarray Based on Digital Images

“…The other detection method is based on the CCD fluorescence scanner. Both fluorescence intensity and fluorescence position of each reaction point could be collected by a CCD camera, whereas the relevant biological information could be obtained through the relevant software analysis [4,5,6]. Moreover, the detection methods of fluorescent labeling are proven complex and difficult to be labeled, which is time-consuming and laborious, whereas the fluorescent markers are significantly expensive, resulting in high costs.…”

Image Processing of Porous Silicon Microarray in Refractive Index Change Detection

“…This allows analysis of the sequence and quantities of target molecules by detection of the intensity distribution of the hybridization signal [5]. Up to now, commercial biological microarray detection has been done by fluorescence and isotope labeling techniques [6,7,8]. However, there are some problems in the detection methods of fluorescent labeling: conventional microarray and sequencing technologies often require several hours of manual operation to complete the labeling process, complex labeling is time-consuming, which leads to high costs, poor reproducibility, and difficulty of use in clinics.…”