A novel, biased-like SDF-1 derivative acts synergistically with starPEG-based heparin hydrogels and improves eEPC migration in vitro

Baumann, Lars; Prokoph, Silvana; Gabriel, Christian; Freudenberg, Uwe; Werner, Carsten; Beck‐Sickinger, Annette G.

doi:10.1016/j.jconrel.2012.04.049

Cited by 61 publications

(38 citation statements)

References 53 publications

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“…Star poly(ethylene glycol) (PEG)-heparin hydrogels were modified by immobilizing a novel, biased-like SDF-1 derivative to mobilize EPCs. 47 The SDF-1 derivative (AAV-[S4V]-SDF-1a) was found to be more effective at increasing EPC migration compared to SDF-1a.…”

Section: Growth Factor Incorporationmentioning

confidence: 95%

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Melchiorri

Hibino

Fisher

2013

Tissue Engineering Part B: Reviews

154

147

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Section: Growth Factor Incorporationmentioning

confidence: 95%

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Melchiorri

Hibino

Fisher

2013

Tissue Engineering Part B: Reviews

154

147

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“…Several heparin-containing cytokine delivery systems have been developed for tissue regeneration and modified to control the release of cytokines. [24][25][26] We have previously published a paper demonstrating that self-assembled heparin/chitosan nanoparticles can load VEGF to stimulate proliferation of endothelial cells in vitro and accelerate vascularization in a murine subcutaneous implant model in vivo. 24 We hypothesized that CSO and heparin could be utilized to develop a novel nanoparticle as a delivery system to load cytokines for tissue regeneration and be stable in a solution with physiological pH.…”

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confidence: 99%

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Wang

Tan

Deng

et al. 2015

IJN

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Background Cell therapy is a promising strategy for tissue regeneration. Key to this strategy is mobilization and recruitment of exogenous or autologous stem/progenitor cells by cytokines. However, there is no effective cytokine delivery system available for clinic application, in particular for myocardial regeneration. The aim of this study was to develop a novel cytokine delivery system that is stable in solution at physiological pH. Methods Four groups of self-assembled chitosan oligosaccharide/heparin (CSO/H) nanoparticles were prepared with various volume ratios of chitosan oligosaccharide to heparin (5:2, 5:4, 4:15, 1:5) and characterized by laser diffraction, particle size analysis, and transmission electron microscopy. The encapsulation efficiency and loading content of two cytokines, ie, stromal cell-derived factor (SDF)-1α and vascular endothelial growth factor (VEGF) were quantified using an enzyme-linked immunosorbent assay. The biological activity of the loaded SDF-1α and VEGF was evaluated using the transwell migration assay and MTT assay. The dispersion profiles for the cytokine-loaded nanoparticles were quantified using fluorescence molecular tomography. Results CSO/H nanoparticles were prepared successfully in solution with physiological pH. The particle sizes in the four treatment groups were in the range of 96.2–210.5 nm and the zeta potential ranged from −29.4 mV to 24.2 mV. The loading efficiency in the CSO/H nanoparticle groups with the first three ratios was more than 90%. SDF-1α loaded into CSO/H nanoparticles retained its migration activity and VEGF loaded into CSO/H nanoparticles continued to show proliferation activity. The in vivo dispersion test showed that the CSO/H nanoparticles enabled to VEGF to accumulate locally for a longer period of time. Conclusion CSO/H nanoparticles have a high cytokine loading capacity and allow cytokines to maintain their bioactivity for longer, are stable in an environment with physiological pH, and may be a promising cytokine delivery system for tissue regeneration.

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“…Chemokines trigger cell migration in the direction of their gradient to home specific cell types to the injured site or the biomaterial surface. This has been employed to recruit endothelial progenitor cells by a SDF-1-gradient, to support neovascularization at injured cardiovascular sites (Baumann et al, 2012). Another beneficial function of SDF-1 is the chemotaxis of mesenchymal stem cells and the stimulation of osteogenic differentiation (Kitaori et al, 2009;Eman et al, 2014).…”

Section: Components Of Multifunctional Biomaterials Coatingsmentioning

confidence: 99%

Multifunctional biomaterial coatings: synthetic challenges and biological activity

Pagel

Beck‐Sickinger

2017

Biological Chemistry

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A controlled interaction of materials with their surrounding biological environment is of great interest in many fields. Multifunctional coatings aim to provide simultaneous modulation of several biological signals. They can consist of various combinations of bioactive, and bioinert components as well as of reporter molecules to improve cell-material contacts, prevent infections or to analyze biochemical events on the surface. However, specific immobilization and particular assembly of various active molecules are challenging. Herein, an overview of multifunctional coatings for biomaterials is given, focusing on synthetic strategies and the biological benefits by displaying several motifs.

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A novel, biased-like SDF-1 derivative acts synergistically with starPEG-based heparin hydrogels and improves eEPC migration in vitro

Cited by 61 publications

References 53 publications

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Multifunctional biomaterial coatings: synthetic challenges and biological activity

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A novel, biased-like SDF-1 derivative acts synergistically with starPEG-based heparin hydrogels and improves eEPC migration in vitro

Cited by 61 publications

References 53 publications

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Strategies and Techniques to Enhance theIn SituEndothelialization of Small-Diameter Biodegradable Polymeric Vascular Grafts

Novel stable cytokine delivery system&nbsp;in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles

Multifunctional biomaterial coatings: synthetic challenges and biological activity

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Novel stable cytokine delivery system in physiological pH solution: chitosan oligosaccharide/heparin nanoparticles