A novel animal model of long-term sustainable anal sphincter dysfunction

Yamaguchi, Izumi; Fujita, Fumihiko; Yamanouchi, Kaoru; Mishima, Takehiro; Kawahara, Daisuke; Sakai, Yusuke; Ito, Shinichiro; Kanetaka, Kengo; Takatsuki, Mitsuhisa; Kuroki, Tamotsu; Eguchi, Susumu

doi:10.1016/j.jss.2013.04.010

Cited by 9 publications

(18 citation statements)

References 24 publications

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“…After anesthesia, all animals underwent sphincterotomy by the removal of a left semicircle of both the IAS and EAS via a posterior incision, as we reported previously [16]. The anal pressure of the ADSC sheet transplantation group is significantly higher than that of the control group (p = 0.05).…”

Section: Surgical Proceduresmentioning

confidence: 80%

“…The anal pressure was recorded just before surgery as a baseline and on postoperative days (PODs) 1, 7, 14, 28, and 56 in all animals, as we reported previously [16]. Under anesthesia, the resting anal pressure was measured using a catheter with a microminiature silicon strain gauge type sensor mounted at one end (CODMAN MICROSENSOR ICP Transducer, Codman, Raynham, MA, USA), connected through a low profile connector (TEC-10D cable, Millar Instruments, Houston, TX, USA), pressure control unit (TCB-500, Millar Instruments), and digital data recording system (PowerLab 4/30, ADInstruments, Nagoya, Japan) as described in our previous study [16].…”

Section: Anal Resting Pressure Measurement and Analysismentioning

confidence: 99%

“…Under anesthesia, the resting anal pressure was measured using a catheter with a microminiature silicon strain gauge type sensor mounted at one end (CODMAN MICROSENSOR ICP Transducer, Codman, Raynham, MA, USA), connected through a low profile connector (TEC-10D cable, Millar Instruments, Houston, TX, USA), pressure control unit (TCB-500, Millar Instruments), and digital data recording system (PowerLab 4/30, ADInstruments, Nagoya, Japan) as described in our previous study [16]. We evaluated the mean pressures at each time point and compared the control group with the ADSC sheet transplantation group.…”

Section: Anal Resting Pressure Measurement and Analysismentioning

confidence: 99%

“…We previously reported an experimental rat model of anal sphincter dysfunction with anal sphincter resection [16], and the goal of this study is to investigate the contribution of ADSC sheets to our previously established animal model.…”

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confidence: 99%

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Improvement of Anal Function by Adipose-Derived Stem Cell Sheets

Inoue

Fujita²,

Yamaguchi³

et al. 2017

Dig Surg

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Aim: One of the most troublesome complications of anal preserving surgery is anal sphincter dysfunction. The aim of this study was to evaluate functional recovery after implantation of adipose-derived stem cell (ADSC) sheets, novel biotechnology, for an anal sphincter resection animal model. Methods: Eighteen female Sprague-Dawley rats underwent removal of the nearest half of the internal and external anal sphincter muscle. Nine rats received transplantation with ADSC sheets to the resected area while the remaining rats received no transplantation. The rats were evaluated for the anal function by measuring their resting pressure before surgery and on postoperative days 1, 7, 14, 28, and 56. In addition, the rats were examined for the presence of smooth muscle and also to determine its origin. Results: The improvement of the anal pressure was significantly greater in the ADSC sheet transplantation group compared with the control group. Histologically, at the vicinity of the remaining smooth muscle, reproduction of smooth muscle was detected. Using in fluorescence in situ hybridization, the cells were shown to be from the recipient. Conclusion: Regenerative therapy using ADSC sheet has the potential to recover anal sphincter dysfunction due to anorectal surgery.

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Section: Surgical Proceduresmentioning

confidence: 80%

Section: Anal Resting Pressure Measurement and Analysismentioning

confidence: 99%

Section: Anal Resting Pressure Measurement and Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Improvement of Anal Function by Adipose-Derived Stem Cell Sheets

Inoue

Fujita²,

Yamaguchi³

et al. 2017

Dig Surg

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“…[9,10] The external sphincter is relevant in preventing urge incontinence [11,12] and contributes to the maintenance of passive continence. [13] However, in previous studies, a variety of methods including sphincterotomy [14,15], resection of the internal and external sphincter [16], electrocoagulation [17] or cryoinjury [18] to generate models for sphincter muscle insufficiency have been used. Therefore data comparison across the literature is difficult.…”

Section: Methodsmentioning

confidence: 99%

A novel animal model for external anal sphincter insufficiency

Brügger

Inglin

Candinas

et al. 2014

Int J Colorectal Dis

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Abstract:Purpose: Reliable animal models are essential to evaluate future therapeutic options like cell-based therapies for external anal sphincter insufficiency. The goal of our study was to describe the most reliable model for external sphincter muscle insufficiency by comparing three different methods to create sphincter muscle damage. Methods: In an experimental animal study female Lewis rats (200-250 g) were randomly assigned to three treatment groups (n=5, each group). The external sphincter muscle was weakened in the left dorsal quadrant by microsurgical excision, cryosurgery, or electrocoagulation by diathermy. Functional evaluation included in vivo measurements of resting pressure, spontaneous muscle contraction, and contraction in response to electrical stimulation of the afferent nerve at baseline and at 2, 4, and 6 weeks after sphincter injury. Mason trichrome staining and immunofluorescence for skeletal muscle markers was performed for morphological analysis. Results: Peak contraction after electrical stimulation was significantly decreased after sphincter injury in all groups. Contraction forces recovered partially after cryosurgery and electrocoagulation but not after microsurgical excision. Morphological analysis revealed an incomplete destruction of the external sphincter muscle in the cryosurgery and electrocoagulation groups compared to the microsurgery group. Conclusions: For the first time three different models of external sphincter muscle insufficiency were directly compared. The animal model using microsurgical sphincter destruction offers the highest level of consistency regarding tissue damage and sphincter insufficiency, and therefore represents the most reliable model to evaluate future therapeutic options. In addition this study represents a novel model to specifically test external sphincter muscle function.Additional Information:Question Response Was this manuscript been published previously or under consideration in wholeThis Manuscript has not been published in whole or in part elsewhere. Powered by Editorial Manager® and ProduXion Manager® from Aries Systems Corporationor in part somewhere else?2. Was this manuscript in its present form has been read and approved to submission to "International Journal of colorectal disease" for publication by all participated authors?This manuscript in its present form has been read and approved to submission to "International Journal of colorectal disease" for publication by all participated authors. Acknowledgments:The authors thank Meline Stoelting, Souzan Salemi, and Fatma Kivrak for the introduction in analyzing techniques and for technical assistance.4. Conflict of interest or financial disclosure:The authors declare no conflict of interest or financial disclosure. Do you certify that this research is entirely original?This research is entirely original.6. Which of the following government agencies provided funding for your research?No government agency provided funding for this research. 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15...

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