A novel animal model for investigating persistent airway hyperresponsiveness

Minshall, Eleanor; Riccio, Maggie M.; Herd, Caroline M.; Douglas, Garry J.; Seeds, Esther A.M.; McKenniff, M.G.; Sasaki, Masahiro; Spina, Domenico; Page, Clive

doi:10.1016/1056-8719(93)90015-7

Cited by 37 publications

(40 citation statements)

References 25 publications

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“…We also compared basal pulmonary function measurements of animals at 3 months of age. The measurements made for RL, Cdyn, respiration rates, tidal volumes and minute volumes compare favourably with values reported previously for the rabbit [19,36]. There were no differences between basal values obtained for RL, Cdyn, maximum TPP or respiration rate between naive rabbits, groups of immunized rabbits exposed to air or SO 2 , air or NO 2 , or HDM-immunized rabbits and their salineinjected controls.…”

Section: Discussionsupporting

confidence: 67%

“…These responses are attenuated by clinically useful anti-asthma drugs [16], and the animals develop 2-6 fold increases in acute airway responsiveness, which resolves in one week [14,15,17,18]. Repeated measurements of lung function are readily made in the rabbit, and each animal may be used as its own control [18,19]. Taking this all together, led us to use the rabbit as the species of choice in our experiments investigating the effects of exposure to pollutant gases from birth.…”

Section: Technical Notementioning

confidence: 99%

“…Pulmonary function measurements were carried out on spontaneously breathing anaesthetized rabbits (2.2-3.1 kg) using a minor modification of the method described previously [19]. Rabbits were first premedicated with diazepam (2.5 mg·kg -1 ), and were subsequently administered Hypnorm (0.4 ml·kg -1 ) to induce neuroleptalgesia.…”

Section: Pulmonary Function Measurementsmentioning

confidence: 99%

“…Histamine challenges were performed on anaesthetized rabbits as described previously [19]. Aerosols of saline and histamine were generated by an ultrasonic nebulizer.…”

Section: Aerosolized Histamine Challengesmentioning

confidence: 99%

“…Rabbits were immunized from birth against Alternaria tenuis according to a previously published protocol [19], and against house dust mite (HDM) antigen using a modification of this method. Rabbits were injected intraperitoneally (i.p.)…”

Section: Immunization Protocolmentioning

confidence: 99%

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A method for the long-term exposure of rabbits to environmental pollutant gases

Douglas

Price²,

Page³

1994

Eur Respir J

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A A m me et th ho od d f fo or r t th he e l lo on ng g--t te er rm m e ex xp po os su ur re e o of f r ra ab bb bi it ts s t to o e en nv vi ir ro on nm me en nt ta al l p po ol ll lu ut ta an nt t g ga as se es s ABSTRACT: The aims of the present study were twofold. Firstly, we wanted to develop a system for the exposure of rabbits to pollutant gases that would monitor gas concentrations accurately, allow flexibility, be simple to operate, and could be constructed at relatively modest cost. Additionally, we wanted to determine whether the procedures necessary for the daily exposure of young rabbits had any detrimental effect on their development. Using the environmental exposure system that we developed, littermate New Zealand White rabbits, neonatally immunized to either Alternaria tenuis or house dust mite antigen were exposed 2 h daily, from within 24 h of birth until 3 months of age, to either 4 ppm nitrogen dioxide (NO 2 ), or 5 ppm sulphur dioxide (SO 2 ) or ambient air.The environmental exposure system consists of four sections; a stainless steel exposure chamber; an airflow monitoring and control system and gas delivery system; a gas detector and monitoring system; and an exhaust fan. Equilibration and wash-out times of gas were short and the gas mixing within the chamber atmosphere was uniform. Levels of gases were reliably maintained throughout the period of exposure within predetermined limits. The weights of the immunized, gas-exposed animals did not differ significantly from those of the immunized, airexposed animals at any time throughout the 3 month period of exposure. At 3 months of age, the basal values for lung resistance and dynamic compliance did not differ between gas-and air-exposed rabbits. These values did not differ significantly from those obtained from naive animals of the same age.Our results suggest that we have developed a sensitive, reliable and simple environmental exposure and monitoring system. It is anticipated that the methodology described will allow the careful investigation of the effects of long-term exposure to pollutant gases from birth on the development of airways hyperresponsiveness.

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Section: Discussionsupporting

confidence: 67%

Section: Technical Notementioning

confidence: 99%

Section: Pulmonary Function Measurementsmentioning

confidence: 99%

“…Histamine challenges were performed on anaesthetized rabbits as described previously [19]. Aerosols of saline and histamine were generated by an ultrasonic nebulizer.…”

Section: Aerosolized Histamine Challengesmentioning

confidence: 99%

Section: Immunization Protocolmentioning

confidence: 99%

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