“…The tissue homogenate was then mixed with a solution of chloroform/methanol (2:1, v/v), according to a ratio of 1:1 (v/v). The prepared sample was centrifuged at 1200 x g for 10 minutes, and the obtained substratum was used for measurement of total cholesterol, triglyceride and free fatty acid according to the colorimetric methods (Lian et al, 2011).…”