A new Zn(II)2Cys6-type transcription factor BglR regulates β-glucosidase expression in Trichoderma reesei

Nitta, Mikiko; Furukawa, Tatsuhiko; Shida, Yosuke; Mori, Kazuki; Kuhara, Satoru; Morikawa, Yasushi; Ogasawara, Wataru

doi:10.1016/j.fgb.2012.02.009

Cited by 134 publications

(144 citation statements)

References 36 publications

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“…26) Among these, one was found in the bglr gene, and it resulted in increased cellulase production in cellobiose medium. 27) Another was found in cre1, and was found to be responsible for catabolite de-repression in PC-3-7. 26) Due to its high cellulase productivity, PC-3-7 is also used industrially to enhance lignocellulosic biomass y To whom correspondence should be addressed.…”

Section: Identification Of Major Facilitator Transporters Involved Inmentioning

confidence: 97%

“…Our previous data showed that cellulase expression in PC-3-7 grown on lactose was about 2-fold greater than in T. reesei QM9414. 27) Morikawa et al (1995) 36) found that the transporters involved in lactose uptake can be induced by the inducers of cellulase, including cellobiose and sophorose. Because lactose is not a component of plant biomass, it is possible that oligosaccharide transporters in T. reesei have evolved to favor induction by cellulose-derived sugars.…”

Section: Identification Of Major Facilitator Transporters Involved Inmentioning

confidence: 99%

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Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Porciúncula

Furukawa

Shida

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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Section: Identification Of Major Facilitator Transporters Involved Inmentioning

confidence: 97%

Section: Identification Of Major Facilitator Transporters Involved Inmentioning

confidence: 99%

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Porciúncula

Furukawa

Shida

et al. 2013

Bioscience, Biotechnology, and Biochemistry

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“…It has been reported that BglR regulates expression of the β-glucosidase gene in T. reesei. 21) Therefore, ClbR might not be involved in regulation of the bgl1 expression in A. aculeatus.…”

Section: Discussionmentioning

confidence: 98%

Effects of clbR overexpression on enzyme production in Aspergillus aculeatus vary depending on the cellulosic biomass-degrading enzyme species

Kunitake

Kawamura

Tani

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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ClbR is a Zn(II)2Cys6 transcriptional activator that controls the expression of cellulase-related genes in response to Avicel and cellobiose in Aspergillus aculeatus. A clbR-overexpressing strain (clbR-OE) that expresses the clbR gene at levels sevenfold higher than the control strain sustainably produced xylanolytic and cellulolytic activities during 10-day cultivation of A. aculeatus, enabling synchronization of xylanolytic and cellulolytic activities at a maximum level. However, clbR overexpression did not simultaneously increase levels of all xylanolytic and cellulolytic enzymes. Peptide mass fingerprint analysis revealed markedly increased production of FIa-xylanase in clbR-OE, whereas expression of FIII-avicelase and FII-carboxymethyl cellulase was unaffected and expression of hydrocellulase was lower in clbR-OE than in the control. Northern blot analysis confirmed that these effects of clbR overexpression on enzyme production were mediated at the transcriptional level. These data suggest that ClbR participates in diverse signaling pathways to control the expression of cellulosic biomass-degrading enzymes in A. aculeatus.

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“…Whilst the expression of a range of CAZyme-encoding genes was partly dependent on AraR, there were few clear examples of CAZyme-encoding genes where the expression was solely dependent on AraR [88]. BglR (beta-glucosidase regulator) positively regulates the expression of genes encoding β-glucosidases in T. reesei [89]. This transcriptional activation could eventually lead to the repression of CAZyme-encoding genes: the β-glucosidases will hydrolyse cellobiose to glucose and, unless the glucose is metabolised by the fungus, repression of CAZyme-encoding genes via CRE1 will occur.…”

Section: Other Relevant Transcription Factorsmentioning

confidence: 99%

Transcriptional Regulation and Responses in Filamentous Fungi Exposed to Lignocellulose

2015

Mycology: Current and Future Developments

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Biofuels derived from lignocellulose are attractive alternative fuels but their production suffers from a costly and inefficient saccharification step that uses fungal enzymes. One route to improve this efficiency is to understand better the transcriptional regulation and responses of filamentous fungi to lignocellulose. Sensing and initial contact of the fungus with lignocellulose is an important aspect. Differences and similarities in the responses of fungi to different lignocellulosic substrates can partly be explained with existing understanding of several key regulators and their mode of action, as will be demonstrated for Trichoderma reesei, Neurospora crassa and Aspergillus spp. The regulation of genes encoding Carbohydrate Active enZymes (CAZymes) is influenced by the presence of carbohydrate monomers and short oligosaccharides, as well as the external stimuli of pH and light. We explore several important aspects of the response to lignocellulose that are not related to genes encoding CAZymes, namely the regulation of transporters, accessory proteins and stress responses. The regulation of gene expression is examined from the perspective of mixed cultures and models are presented for the nature of the transcriptional basis for any beneficial effects of such mixed cultures. Various applications in biofuel technology are based on manipulating transcriptional regulation and learning from fungal responses to lignocelluloses. Here we critically access the application of fungal transcriptional responses to industrial saccharification reactions. As part of this chapter, selected regulatory mechanisms are also explored in more detail.

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A new Zn(II)2Cys6-type transcription factor BglR regulates β-glucosidase expression in Trichoderma reesei

Cited by 134 publications

References 36 publications

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Identification of Major Facilitator Transporters Involved in Cellulase Production during Lactose Culture ofTrichoderma reeseiPC-3-7

Effects of clbR overexpression on enzyme production in Aspergillus aculeatus vary depending on the cellulosic biomass-degrading enzyme species

Transcriptional Regulation and Responses in Filamentous Fungi Exposed to Lignocellulose

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