A new turbidimetric immunoassay for serum calprotectin for fully automatized clinical analysers

Abstract

Serum and plasma calprotectin concentration is shown to be elevated when neutrophils are activated, and may therefore be used as a marker for inflammatory diseases. A serum calprotectin immunoassay was developed based on calprotectin values observed in samples from the intensive care unit. The polyclonal avian antibodies were raised and affinity purified with calprotectin antigens. The performance was tested and it was observed that the assay was linear in the range 0.3–24.7 mg/L, the limit of quantitation was observed to be lower than 0.3 mg/L, no antigen excess was observed up to 54 mg/L, all CVs were lower than 1.8 % in the precision study, the calibration curve stability was longer than 6 weeks, and there was no significant interference detected for haemoglobin, intralipid or bilirubin. The serum calprotectin immunoassay presented in this paper performs well within the criteria carefully set from the limited clinical experience obtained in both serum and plasma. In addition it is commutable with Bühlmann MRP8/14 ELISA.

Polycystic Ovary Syndrome and Insulin Physiology: An Observational Quantitative Serum Proteomics Study in Adolescent, Normal‐Weight Females

“…[21] Serum S100A8 levels have been shown to increase in inflammatory disease, such as autoimmune disease (including rheumatoid arthritis and inflammatory bowel disease), various types of cancer, cystic fibrosis, and neurodegeneration. [22][23][24] In our study, serum S100A8 levels were higher in females with PCOS compared to controls.…”

“…Calgranulin A (S100A8) is a low molecular weight protein with calcium binding properties that is released by activated granulocytes and mediates inflammatory signaling pathways, such as the NF‐kB pathway . Serum S100A8 levels have been shown to increase in inflammatory disease, such as autoimmune disease (including rheumatoid arthritis and inflammatory bowel disease), various types of cancer, cystic fibrosis, and neurodegeneration . In our study, serum S100A8 levels were higher in females with PCOS compared to controls.…”

Fecal calprotectin: beyond intestinal organic diseases

“…More recently, a novel particle enhanced turbidimetric immunoassay (PETIA) for FC quantitation have been proposed as a less expensive and a less labor intensive alternative method to traditional ELISA. 48 Turbidimetry measure the loss of intensity of transmitted light due to the scattering effect of particles in a solution. 49 The peculiarity of PETIA method is that can be applied on standard chemistry analyzers.…”

Extraction, isolation, and concentration of calprotectin antigen (S100A8/S100A9) from granulocytes

“…No. 1201 and 1251), as described in Nilsen et al, on the automatic clinical chemistry analyser, Mindray BS‐380 (Mindray, Shenzhen, Kina). The fractions with high calprotectin levels were pooled.…”