A new study of the bacterial lipidome: HPTLC-MALDI-TOF imaging enlightening the presence of phosphatidylcholine in airborne Pseudomonas fluorescens MFAF76a

Glycerophospholipid synthesis and functions in Pseudomonas

“…Integral cellular analysis gives the composition of about 75% PE and 20% PG. Remainders are represented by cardiolipin (CL) (Diedrich and Cota-Robles, 1974) and phosphatidylcholine (PC), recently found in Pseudomonas strains (Boeris and Lucchesi, 2012;Geiger et al, 2013;Kondakova et al, 2014). CL is accumulated in Pseudomonas spp.…”

“…Recently, strains of P. syringae, virulent on diverse species of crop plants, were isolated from epilithic biofilms of rivers (Morris et al, 2007) and clouds at several kilometers altitude (Amato et al, 2007) outside the zones of agricultural production (Morris et al, 2008). Bacteria of the P. fluorescens group commonly found in soil (Bodilis et al, 2006) and water (Janek et al, 2010) are also able to develop in air Kondakova et al, 2014). Some of P. fluorescens group members, adapted to the human temperature are members of the skin microbiota or behave as opportunistic pathogens (Chapalain et al, 2008).…”

Direct Coupling of HPTLC with MALDI‐TOF MS for Qualitative Detection of Flavonoids on Phytochemical Fingerprints

“…Until now, this approach has been successfully applied for the analysis of various types of molecules e.g. lipids in (i) stem cells (Fuchs et al , ), (ii) soybean and sunflower (Krüger et al , ), and (iii) bacteria (Kondakova et al , ), oligosaccharides (Nimptsch et al , ), as well as synthetic materials (Leriche et al , ). Although conventional MALDI‐TOF MS is commonly used for analysis of flavonoids (Wang and Sporns, ; Frison‐Norrie and Sporns, ; Grant and Helleur, ), to the best of our knowledge no reports of MALDI MS analysis of flavone glycosides and flavonol glycosides and their corresponding aglycones directly from TLC plates have been made to date.…”

Recent advances in the preparation of adsorbent layers for thin‐layer chromatography combined with matrix‐assisted laser desorption/ionization mass‐spectrometric detection

“…In Ref. , the first adaptation of the HPTLC–MALDI TOF MS to the study of the bacterial lipidome was described. The authors discussed an analytical method coupling HPTLC to MALDI TOF MS for bacterial lipid analysis, fragmentation, and identification.…”