A new strain of potato spindle tuber viroid (PSTVd-N) exhibits major sequence differences as compared to all other PSTVd strains sequenced so far

Puchta, Holger; Herold, Thomas; Verhoeven, J. Th. J.; Roenhorst, Annelien; Ramm, Karla; Schmidt-Puchta, Waltraud; Sänger, Heinz L.

doi:10.1007/bf00019169

Cited by 52 publications

(36 citation statements)

References 20 publications

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“…Transmission could have occurred by mechanical contact with breeding stock or, because PSTVd is vertically transmissible in some hosts (17)(18)(19), through the (true) seed or pollen from infected wild plants. PSTVd variants have indeed been identified in several major germplasm collections, but the source of these viroids cannot be traced back to wild solanaceous plants (20)(21)(22)(23). The cultivated potato (Solanum tuberosum L.) originated in the Andes of South America (24), but a search for the presence of PSTVd (or of similar viroids) in more than 5000 specimens of Andean solanaceous plants, including some known to have been used in potato breeding, remained fruitless (22).…”

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confidence: 99%

Mexican papita viroid: putative ancestor of crop viroids.

Martı́nez-Soriano

Galindo-Alonso²,

Maroon³

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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The potato spindle tuber disease was first observed early in the 20th century in the northeastern United States and shown, in 1971, to be incited by a viroid, potato spindle tuber viroid (PSTVd). No wild-plant PSTVd reservoirs have been identified; thus, the initial source of PSTVd infecting potatoes has remained a mystery. Several variants of a novel viroid, designated Mexican papita viroid (MPVd), have now been isolated from Solanum cardiophyllum Lindl. (papita güera, cimantli) plants growing wild in the Mexican state of Aguascalientes. MPVd's nucleotide sequence is most closely related to those of the tomato planta macho viroid (TPMVd) and PSTVd. From TPMVd, MPVd may be distinguished on the basis of biological properties, such as replication and symptom formation in certain differential hosts. Phylogenetic and ecological data indicate that MPVd and certain viroids now affecting crop plants, such as TPMVd, PSTVd, and possibly others, have a common ancestor. We hypothesize that commercial potatoes grown in the United States have become viroid-infected by chance transfer of MPVd or a similar viroid from endemically infected wild solanaceous plants imported from Mexico as germplasm, conceivably from plants known to have been introduced from Mexico to the United States late in the 19th century in efforts to identify genetic resistance to the potato late blight fungus, Phytophthora infestans.

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mentioning

confidence: 99%

Mexican papita viroid: putative ancestor of crop viroids.

Martı́nez-Soriano

Galindo-Alonso²,

Maroon³

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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“…The first exception to this presumed rule became the recently sequenced PSTVd-N from pepino (Solanum muricatum) plants [ 15]. PSTVd-N was found to exhibit not only major sequence differences as compared to all other sequenced PSTVd strains from potato, but also the length of its RNA chain differs in that it is 356 nt long and thus 3 nt shorter than the presumed 'unit length' of P STVd [15].…”

mentioning

confidence: 99%

“…PSTVd-N was found to exhibit not only major sequence differences as compared to all other sequenced PSTVd strains from potato, but also the length of its RNA chain differs in that it is 356 nt long and thus 3 nt shorter than the presumed 'unit length' of P STVd [15]. It was tacitly assumed that these gross differences reflected the adaptation of PSTVd-N to the host plant pepino.…”

mentioning

confidence: 99%

“…Total nucleic acids were phenolextracted from the infected tomato leaves, and the viroid RNA was separated from the cellular lowmolecular-weight RNAs by polyacrylamide gel electrophoresis (PAGE) using the 'bidirectional' method [22]. The separated circular viroid RNA was isolated from the gel [28] and reversely transcribed into overlapping cDNA as previously described [12,15,20,27]. To initiate cDNA synthesis the following three [32p]-end-labelled PSTVd-specific DNA primers were used: p M T S E 2 (5'-dGCCGCTGGGC ACTCCCC-ACC GTCCTTTTTT GCCAG-3'), pMTSE 3 (5'-GAGGTCAGGT GTGAACCACA-3') and pMTSE5 (5'-dTTGTTTCCAC CGGGTAG-TAG-3').…”

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confidence: 99%

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Sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (PSTVd) is not strictly conserved but as variable as in other viroids

Herold

Haas

Singh³

et al. 1992

Plant Mol Biol

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The sequence analysis of five new field isolates of potato spindle tuber viroid (PSTVd) of different virulence revealed that the length of their RNA chain is not strictly conserved to 359 nucleotides (nts), as one could have inferred from the previously sequenced PSTVd strains. It was now found that the chain length is strain-specific like in the case of practically all other viroids, and that it may vary, so far, between 356 and 360 nts. Taking our previously sequenced and least virulent mild strain PSTVd KF6-M as standard, the new mild strains PSTVd WA-M and PSTVd F-M differ from it by one or two nts. The new intermediate-severe strains PSTVd F-IS and PSTV-F 88-IS differ from the standard mild strain by eight and nine nts, respectively, whereas the new severe-lethal strain PSTVd F-SL differs in seven nts. Most of these mutations are located within the virulence-modulating (VM) region and within the variable region (VR), and only in two strains a single mutation is found in the right terminal domain.

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“…El PSTVd se ha detectado en Australia (Walter y McLeod, 1982), Brasil (Ávila et al, 1990), Canadá (Singh y Crowley, 1985;Singh et al, 1988), Chile (Shamloul et al, 1997), China (Tien, 1985;Singh et al, 1991), Costa Rica (Badilla et al, 1999), Grecia (Puchta et al, 1990), Reino Unido (Harris et al, 1979), Estados Unidos (Martin, 1922;Diener, 1971a;Singh y Clark, 1971), India , Nueva Zelanda (Puchta et al, 1990) y Rusia (Huttinga et al, 1987). Sin embargo, su distribución geográfica actual es limitada a causa de las estrictas medidas adoptadas por los países desarrollados para su control y erradicación (Singh et al, 2003a).…”

Section: Corteunclassified

Resistencia a viroides inducida por ribozimas de cabeza de martillo y RNAs interferentes

Olivares¹

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A new strain of potato spindle tuber viroid (PSTVd-N) exhibits major sequence differences as compared to all other PSTVd strains sequenced so far

Cited by 52 publications

References 20 publications

Mexican papita viroid: putative ancestor of crop viroids.

Mexican papita viroid: putative ancestor of crop viroids.

Sequence analysis of five new field isolates demonstrates that the chain length of potato spindle tuber viroid (PSTVd) is not strictly conserved but as variable as in other viroids

Resistencia a viroides inducida por ribozimas de cabeza de martillo y RNAs interferentes

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