A new spin label method for the measurement of erythrocyte internal microviscosity

Daveloose, Denis; Fabre, G.; Berleur, François; Testylier, Guy; Leterrier, F

doi:10.1016/0167-4889(83)90023-x

Cited by 22 publications

(16 citation statements)

References 19 publications

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“…2) can also be explained in spite of the polymerization process that takes place in these samples under our experimental conditions. We state asa hypothesis that the insensitivity of the r/~ value around 4MT plus Glu to the interactions among the macromolecules is related to the small volume of this probe (0.033.10 -19 cm 3) [11] with respect to Hb (1.02.10 -19 cm 3) [9]. Its volume is approximately 31 times smaller than that of Hb, which could cause it to remain in the solvent without being affected by the interactions among the Hb molecules, a fundamental element during HbS polymerization.…”

Section: Discussionmentioning

confidence: 99%

EPR study of the hemoglobin rotational correlation time and microviscosity during the polymerization of hemoglobin S

et al. 2006

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Abstraet. The microviscosity and the protein rotational correlation time are analyzed in samples of hemoglobin A and hemoglobin S with the intracellular concentration at 36 •C and during spontaneous deoxygenation. With this purpose, we use glutathione and carbonmonoxy hemoglobin labeled with 4-maleimido-2,2,6,6-tetramethyl-piperidine-l-oxyl (TEMPO) as probes and 4-maleimido TEMPO bound to the hemoglobin (A and S) asa spin label. The saturation transfer electron paramagnetic resonance experiment showed a sigmoidal behavior, and an increase (about twice) of the hemoglobin rotational correlation time and microviscosity during the polymerization process of hemoglobin S. The delay time determined by this method coincides with that obtained in proton magnetic resonance experiments. These results help to explain the temporal behavior of the proton relaxation times obtained in samples of hemoglobin A and S under the same experimental eonditions.

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Section: Discussionmentioning

confidence: 99%

EPR study of the hemoglobin rotational correlation time and microviscosity during the polymerization of hemoglobin S

et al. 2006

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“…The high intracellular red cell viscosity causes a decrease in h l/h,, associated with reduced rates of tumbling, and a concomitant increase in the rotational correlation time (Daveloose et al, 1983;Morse, 1985). According to the Kivelson theory, increasing viscosity should increase the width and decrease the height of each ESR line (Kivelson, 1960); this is only true if there is no line broadening from collisional self-quenching.…”

Section: ]~Quilibrium Volumesmentioning

confidence: 97%

“…In this paper we show that electron spin resonance (ESR) techniques can be used effectively to measure red cell volume transients as well as equi-librium volumes. Earlier studies using the spin probes tempamine and MAL-3 measured changes in the microviscosity of red cells by monitoring the probe correlation times (Daveloose et al, 1983;Morse, 1985). Similar measurements were made on solutions of normal and sickle hemoglobin with and without oxygenation using the probe Tempone (Beaudoin & Mizukami, 1978).…”

Section: Introductionmentioning

confidence: 96%

ESR measurement of time-dependent and equilibrium volumes in red cells

et al. 1990

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Red cell water volumes were measured using ESR methods during transient osmotic perturbation, and under equilibrium conditions. Cell water contents were determined using the spin label Tempone (2,2,6,6-tetramethyl piperidine-N-oxyl) and the membrane impermeable quencher potassium chromium oxalate. With appropriate corrections for intracellular viscosity and changes in cavity sensitivity, equilibrium cell water measured both by electron spin resonance (ESR) and wet minus dry weight methods gave excellent agreement in solutions from 243-907 mOsm. Intracellular viscosities determined from the Tempone correlation times in the same cells gave values ranging from 9-47 centipoise at 21 degrees C. Osmotically induced transient volume changes were measured using Tempone and an ESR stopped-flow configuration. The Tempone response time was estimated at 17 msec compared to 250-350 msec for normal water relaxations. Nonlinear least square solutions to the Kedem-Katchalsky equations including a correction for the finite Tempone permeability gave 0.029 and 0.030 cm/sec for the osmotic permeability of RBCs in swell and shrink experiments, respectively. In stopped-flow experiments accurate water flux data are obtained very soon after challenging cells and do not require baseline subtractions. These results represent significant improvements over conventional light scattering techniques which necessitate corrections for long lasting optical artifacts (200-300 msec), and baseline drifts.

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“…Its correlation time τ gives information of RBC internal microviscosity. [9] Our results showed that the treatment of RBCs with Al(III) 10 µM decreased the internal microviscosity (Fig. 7).…”

Section: Discussionmentioning

confidence: 55%

In vitro effect of aluminium upon erythrocyte membrane properties

Hernández

Bollini

Huarte

et al. 2008

Clinical Hemorheology and Microcirculation

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The link between aluminium (Al(III)) and a range of disorders in organisms (plants and animals including human beings) has been stated in diverse studies. As regards as human beings in particular, there are numerous studies on this metal's toxicity in relation to pathological processes. Only few references to the metal's effect upon cell rheological properties can be found. In this study, we present evidence for alterations in the rheological properties of cells as consequence of the Al(III)'s interaction with human red blood cell membrane. Al(III) could damage membrane functions of the red blood cell by favouring lipid peroxidation reactions due to the presence of Fe(II) as an initiator. The metal's effect on lipid bilayer, and probably on the cytoskeleton as well, would constitute the cause for the impaired erythrocyte rheology.

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A new spin label method for the measurement of erythrocyte internal microviscosity

Cited by 22 publications

References 19 publications

EPR study of the hemoglobin rotational correlation time and microviscosity during the polymerization of hemoglobin S

EPR study of the hemoglobin rotational correlation time and microviscosity during the polymerization of hemoglobin S

ESR measurement of time-dependent and equilibrium volumes in red cells

In vitro effect of aluminium upon erythrocyte membrane properties

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