A new screening for hemagglutinins from Vietnamese marine macroalgae

Abstract

Aqueous extracts from 42 species of Vietnamese marine macroalgae, including 17 Chlorophyta, 22 Rhodophyta, and three Phaeophyta species, were examined for hemagglutination activity using native and enzyme-treated different animal and human erythrocytes. All extracts agglutinated at least one type of erythrocytes tested. Strong activity was detected in extracts from four Chlorophyta (Caulerpa serulata var. boryana, Caulerpa sertularioides f. longipes, Halimeda velasquezii, and Halimeda discoidea) and two Rhodophyta species (Gelidiella acerosa and Titanophora pulchra) with enzyme-treated rabbit and horse erythrocytes. The hemagglutinins of some active species were examined for sugar-binding specificity, pH, temperature stability, and divalent cation independency using ammonium sulfate precipitates prepared from their extracts. In a hemagglutination-inhibition test with various monosaccharides and glycoproteins, none of the hemagglutinins had affinity for monosaccharides. The activity of the hemagglutinins was inhibited by some glycoproteins tested. The inhibition profiles with glycoproteins were different depending on hemagglutinin species, suggesting the presence of lectins specific for complex N-glycans, high mannose N-glycans or O-glycans. On the other hand, the activities of almost all algal hemagglutinins were stable over a wide range of pH and temperature, and independent of the presence of divalent cations, except Gelidiopsis scoparia hemagglutinin, its activity was dependent on the presence of divalent cations. These results suggest that Vietnamese marine macroalgae may be good sources of useful lectins for many biological applications.

Extraction and Partial Characterization of Lectin from Indonesian Brown Algae Padina australis and Padina minor

“…However, we found that the HA of P. minor was stable on a wide range of pH and temperature. Lectin of Dictyospheria versluysii green algae was also reported stable in a wide range of temperature and pH (Hung et al, 2012). The HA of P. minor protein extract was not affected by the presence of chelating agent EDTA, indicating that it did not require divalent cation to maintain its biological activity (Oliveira et al, 2002).…”

“…However, up till now, report on brown algae lectin is still limited. Only few publications reported the screening of brown algae lectin (Anam, Praseptiangga, Fajarningsih, & Intaqta, 2016;Fajarningsih, Intaqta, Praaseptiangga, Anam, & Chasanah, 2018;Fajarningsih et al, 2015;Freitas et al, 1997;Hung et al, 2012) and to our knowledge only one publication reported the purification of brown algae lectin, an HFL lectin isolated from Hizikia fusiformis (Wu, Tong, Wu, Liu, & Li, 2016). According to Harrysson et al (2018), isolation of lectin or other protein from brown algae is difficult due to the high content of non-protein interfering compounds such as polyphenols and viscous polysaccharide.…”

Penapisan Senyawa Hemagglutinin dari Makroalga Asal Pantai Binuangeun, Banten, Indonesia

“…Hemagglutinin, aglutinin atau lektin merupakan protein non imun alami yang mengikat karbohidrat secara reversible dan mempunyai kemampuan dalam mengaglutinasi sel atau mengikat polisakarida dan glikoprotein (Goldstein et al, 1980;Le Hung et al, 2012). Penekanan pada non-imun, dimaksudkan untuk membedakan lektin dari antibodi-karbohidrat tertentu, seperti kinase, glicosidase, transferase ataupun transporter (Goldstein et al, 1980).…”

“…Dapat dilihat pada Tabel tersebut, eritrosit kelinci lebih sensitif untuk mendeteksi adanya aktivitas hemagglutinasi dibandingkan dengan eritrosit manusia. Berdasarkan penelitian Le Hung et al (2012), eritrosit kelinci memiliki sensitivitas paling tinggi untuk mendeteksi aktivitas hemagglutinasi lektin makroalga, kemudian disusul oleh eritrosit domba, ayam, manusia (A,B,O) dan kuda. Hasil tersebut, sedikit berbeda dengan yang dilaporkan oleh Freitas et al (1997) yang menunjukkan bahwa eritrosit domba justru paling sensitif untuk mendeteksi aktivitas hemagglutinasi ekstrak alga yang dipelajari.…”

“…Sementara itu, di antara keempat ekstrak makroalga merah yang diuji pada penelitian ini, hanya ekstrak L. tronoi yang mampu menghemagglutinasi golongan darah A (native). Hasil ini sedikit berbeda dengan yang dilaporkan Le Hung et al (2012), di mana beberapa sampel makroalga dari genus Laurencia hanya aktif menghemagglutinasi eritrosit kelinci. Ekstrak TBS Gracilaria lichenoides dan ekstrak TBS & PBS Gelidiella acerosa dapat menghemagglutinasi semua eritrosit yang diujikan.…”