A new quantitative LC tandem mass spectrometry assay for serum 25-hydroxy vitamin D

Herrmann, Markus; Harwood, D. Tim; Gaston-Parry, Olivia; Kouzios, Dorothy; Wong, Tak Ming; Lih, Anna; Jimenez, Mark; Janu, Margaret R.; Seibel, Markus J.

doi:10.1016/j.steroids.2010.07.006

Cited by 32 publications

(19 citation statements)

References 21 publications

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“…The outstanding performance of the DiaSorin RIA confirmed the findings of a previous study (12 ) and was most likely attributable to the acetonitrile extraction step used in this assay. This step released all 25OH-D from VDBP and eliminated important sources of interference, including heterophile antibodies, before incubation with the capture antibody.…”

Section: Distribution) Which Demonstrated Interlaboratory Cvs For Lcsupporting

confidence: 84%

“…Unfortunately, the poor precision of the Roche test at the higher concentration could not be reevaluated owing to discontinuation of this test between the 2 precision experiments. The poor precision seen with the Roche assay may be specific to the analyzer used in our study, because a previous study had shown excellent precision for this test (12 ). During the first experiment the LIAISON assay also showed suboptimal precision for the low serum pool but improved markedly during the second experiment.…”

Section: Distribution) Which Demonstrated Interlaboratory Cvs For Lcmentioning

confidence: 71%

“…Automated 25OH-D immunoassays from various manufacturers including DiaSorin and Roche Diagnostics have been available for some time, but until recently the accuracy and precision of some of these tests were unsatisfactory (9,(11)(12)(13). There are 2 main difficulties in establishing an automated immunoassay for 25OH-D:…”

Section: -Hydroxy Vitamin D (25oh-d)mentioning

confidence: 99%

“…This is of particular relevance in regard to homogenous 1-step assays, in which 25OH-D and VDBP are not completely separated. Such assays have been shown to agree poorly with higher-order methods, such as LC-MS/MS (12 ).…”

Section: -Hydroxy Vitamin D (25oh-d)mentioning

confidence: 99%

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State-of-the-Art Vitamin D Assays: A Comparison of Automated Immunoassays with Liquid Chromatography–Tandem Mass Spectrometry Methods

Farrell¹,

Martin²,

et al. 2012

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Section: Distribution) Which Demonstrated Interlaboratory Cvs For Lcsupporting

confidence: 84%

Section: Distribution) Which Demonstrated Interlaboratory Cvs For Lcmentioning

confidence: 71%

Section: -Hydroxy Vitamin D (25oh-d)mentioning

confidence: 99%

Section: -Hydroxy Vitamin D (25oh-d)mentioning

confidence: 99%

See 2 more Smart Citations

State-of-the-Art Vitamin D Assays: A Comparison of Automated Immunoassays with Liquid Chromatography–Tandem Mass Spectrometry Methods

Farrell¹,

Martin²,

et al. 2012

Self Cite

View full text Add to dashboard Cite

“…Notably, there were differences in the data obtained from LC/MS/MS 25OHD measurement compared with CLIA, wherein the 25OHD levels were higher with the LC/MS/MS measure. These differences are likely caused by differences in assay performance, which have been previously reported (50). Notably, the majority of subjects (60/90) still had 25OHD levels #20 ng/ml as measured by LC/MS/MS; 27 of 90 subjects had 25OHD levels .20 and #30 ng/ml by LC/MS/MS.…”

Section: Discussionmentioning

confidence: 74%

Randomized Trial Assessing the Effects of Ergocalciferol Administration on Circulating FGF23

Burnett-Bowie

Leder

Henao

et al. 2012

Clinical Journal of the American Society of Nephrology

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SummaryBackground and objectives Fibroblast growth factor 23 is a phosphate-and vitamin D-regulating hormone. The objective of this study was to determine the effect of ergocalciferol administration on fibroblast growth factor 23 levels in healthy vitamin D-deficient subjects.Design, setting, participants, & measurements In this 12-week trial conducted in a clinical research center, 18-to 45-year-old subjects (n=90) with 25-hydroxyvitamin D levels #20 ng/ml (by chemiluminescent immunoassay) were randomized to weekly ergocalciferol treatment of 50,000 international units or placebo, while consuming a self-selected diet. Changes in fibroblast growth factor 23, 25-hydroxyvitamin D (by liquid chromatography/ tandem mass spectroscopy), 1,25-dihydroxyvitamin D, parathyroid hormone, and serum phosphate were measured.Results Mean 25-hydroxyvitamin D (P,0.0001), 1,25-dihydroxyvitamin D (P=0.01), and fibroblast growth factor 23 (P=0.003) increased in the treatment versus placebo group. In the treatment group, 25-hydroxyvitamin D increased from 1867 to 40612 ng/ml at week 4 (P,0.0001) and remained stable at 43612 ng/ml at week 12 (P,0.0001); 1,25-dihydroxyvitamin D increased from 42617 to 52618 pg/ml at week 4 (P,0.001) and then remained stable, and fibroblast growth factor 23 increased from 43617 to 60633 pg/ml at week 8 (P=0.001) and 74642 pg/ml at week 12 (P,0.0001). Urinary phosphate excretion increased within the treatment group, but parathyroid hormone and serum phosphate were unchanged.Conclusions Ergocalciferol administration increases circulating fibroblast growth factor 23. When measuring fibroblast growth factor 23, concurrent 25-hydroxyvitamin D measurements should be obtained, because vitamin D deficiency may lower circulating fibroblast growth factor 23 levels.

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Biomarkers for Vitamin Status and Deficiency

Zhang

Crowther

2017

Targeted Biomarker Quantitation by LC–MS

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A new quantitative LC tandem mass spectrometry assay for serum 25-hydroxy vitamin D

Cited by 32 publications

References 21 publications

State-of-the-Art Vitamin D Assays: A Comparison of Automated Immunoassays with Liquid Chromatography–Tandem Mass Spectrometry Methods

State-of-the-Art Vitamin D Assays: A Comparison of Automated Immunoassays with Liquid Chromatography–Tandem Mass Spectrometry Methods

Randomized Trial Assessing the Effects of Ergocalciferol Administration on Circulating FGF23

Biomarkers for Vitamin Status and Deficiency

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