A new polyphosphate calcium material with morphogenetic activity

Müller, Werner; Tolba, Emad; Schröder, Heinz C.; Wang, S. F.; Glaßer, Gunnar; Muñoz‐Espí, Rafael; Link, Thorben; Wang, X. H.

doi:10.1016/j.matlet.2015.02.070

Cited by 95 publications

(191 citation statements)

References 24 publications

(23 reference statements)

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“…6,[34][35][36] PolyP-mediated modulation of osteoblast-specific gene expression has been studied in MC3T3E1, SaOS2 and MSCs. 20,34,37,38 To the best of our knowledge, there were no reports available to support the role of Pg and its composite scaffolds in bone formation. In this study, we developed CS-based scaffolds and tested the components, CaPP and Pg particles, for their ability to promote bone formation in vitro and in vivo.…”

Section: Discussionmentioning

confidence: 99%

Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering

et al. 2017

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Objectives: Treatment of critical-sized bone defects with cells and biomaterials offers an efficient alternative to traditional bone grafts. Chitosan (CS) is a natural biopolymer that acts as a scaffold in bone tissue engineering (BTE). Polyphosphate (PolyP), recently identified as an inorganic polymer, acts as a potential bone morphogenetic material, whereas pigeonite (Pg) is a novel iron-containing ceramic. In this study, we prepared and characterized scaffolds containing CS, calcium polyphosphate (CaPP) and Pg particles for bone formation in vitro and in vivo.

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Section: Discussionmentioning

confidence: 99%

Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering

et al. 2017

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“…The fabrication of amorphous Ca-phosphate microparticles (aCa-polyP-MP) was performed as previously described (Müller et al, 2015b;Müller et al, 2015d). In short, 2.8 g of CaCl 2 ·2 H 2 O (#223506, Sigma-Aldrich, Taufkirchen, Germany) were dissolved in 25 mL of distilled water and added dropwise to 1 g of Na-polyP in 25 mL distilled water at room temperature.…”

Section: Preparation Of Polyp Microparticlesmentioning

confidence: 99%

“…The patterns were registered on a Philips PW 1820 diffractometer (Philips, Eindhoven; the Netherlands) with CuKα radiation (λ = 1.5418 Å, 40 kV, 30 mA) in the range 2θ = 5-65° (Δ2θ = 0.02, Δt = 5 s). Fourier transformed infrared spectroscopic (FTIR) analyses were performed with micromilled powder in an ATR (attenuated total reflectance)-FTIR spectroscope/Varian 660-IR spectrometer (Agilent, Santa Clara, CA, USA), fitted with a Golden Gate ATR unit (Specac, Orpington, UK), as previously described (Müller et al, 2015b).…”

Section: Preparation Of Polyp Microparticlesmentioning

confidence: 99%

“…However, polyP is not present in the crystalline form in intact cells but as an amorphous polymer (Morrissey et al, 2012). In order to approach the physiological situation in a biomimetic way, we introduced a fabrication process that allows the preparation of amorphous microparticles of polyP (Müller et al, 2015b). Initially, those particles have been prepared with Ca 2+ as the counterion to the phosphate groups within the polyP.…”

Section: Introductionmentioning

confidence: 99%

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Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

Wang¹,

Ackermann²,

Tolba³

et al. 2016

eCM

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Here we show that inorganic polyphosphate (polyP), a polyanionic metabolic regulator consisting of multiple phosphate residues linked by energy-rich phosphoanhydride bonds, is present in the synovial fluid. In a biomimetic approach, to enhance cartilage synthesis and regeneration, we prepared amorphous polyP microparticles with Mg 2+ as counterions. The particles were characterised by X-ray diffraction (XRD), energy-dispersive X-ray (EDX) and Fourier transformed infrared spectroscopic (FTIR) analyses. Similar particles were obtained after addition of Mg 2+ ions to a solution containing hyaluronic acid, as a major component of the synovial fluid, and soluble NapolyP. The viscous paste-like material formed, composed of globular microparticles with diameter of 400 nm, strongly promoted the adhesion of chondrocytes and caused a significant upregulation of the expression of the genes encoding collagen type 3A1, as a marker for chondrocyte differentiation, and SOX9, a transcription factor that regulates chondrocyte differentiation and proliferation. The expression level of the collagen type 3A1 gene was also enhanced by exposure of chondrocytes to synovial fluid that was found to contain polyP with a size of about 80 phosphate residues. This stimulatory effect was abolished after pre-incubation of the synovial fluid with the polyP degrading alkaline phosphatase. We propose a strategy for treatment of joint dysfunctions caused by osteoarthritis based on the application of amorphous Mg 2+ -polyP microparticles that prevent calcium crystal formation in the synovial fluid using scavenging Ca -polyP to hyaluronic acid at the cartilage surface.

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“…This approach has already been used by several groups to assess the mechanical properties of tissues and cells. 11,[31][32][33][34][35][36][37][38] Furthermore, in 2013, we have demonstrated that the ferrule-top probes used for indentation can be modi¯ed to host another optical ber that, connected with an OCT system, enables the user to look at how the subsurface features of a sample deform when the indenter is pushed into the sample. 39 Those studies have never been brought beyond a¯rst proof-of-concept, whose practical relevance in life sciences was very limited: the use of new fabrication techniques and materials lead to a reduction of the sti®ness of the cantilever by three orders of magnitude with respect to those initial studies, enabling measurements on biologicallyrelevant soft materials.…”

Section: Introductionmentioning

confidence: 99%

Multimodal probe for optical coherence tomography epidetection and micron-scale indentation

Bartolini

Feroldi

Weda

et al. 2017

J. Innov. Opt. Health Sci.

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We present a multimodal ferrule-top sensor designed to perform the integrated epidetection of Optical Coherence Tomography (OCT) depth-pro¯les and micron-scale indentation by all-optical detection. By scanning a sample under the probe, we can obtain structural cross-section images and identify a region-of-interest in a nonhomogeneous sample. Then, with the same probe and setup, we can immediately target that area with a series of spherical-indentation measurements, in which the applied load is known with a N precision, the indentation depth with sub-m precision and a maximum contact radius of 100 m. Thanks to the visualization of the internal structure of the sample, we can gain a better insight into the observed mechanical behavior. The ability to impart a small, con¯ned load, and perform OCT A-scans at the same time, could lead to an alternative, high transverse resolution, Optical Coherence Elastography (OCE) sensor.

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A new polyphosphate calcium material with morphogenetic activity

Cited by 95 publications

References 24 publications

Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering

Proliferation and differentiation of mesenchymal stem cells on scaffolds containing chitosan, calcium polyphosphate and pigeonite for bone tissue engineering

Artificial cartilage bio-matrix formed of hyaluronic acid and Mg2+-polyphosphate

Multimodal probe for optical coherence tomography epidetection and micron-scale indentation

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