A new polymorphic determinant on HLA-DQ molecules

Shipp, Margaret A.; Ahmed, Parveen; Kannapell, Carol C.; Ford, Jeanne C.; McCourt, David W.; Leykam, Joseph F.; Zacheis, M.; Bono, Chris; Davie, Joseph M.; Nahm, Moon H.; Schwartz, Benjamin D.

doi:10.1016/0198-8859(86)90033-9

Cited by 3 publications

(1 citation statement)

References 23 publications

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“…Hybridoma cells secreting the anti-MHC class I monoclonal antibody W6/32 (Parham et al, 1979) were obtained from ATCC. Hybridoma cells secreting the anti-MHC class II monoclonal antibodies HK14 (Shipp et al, 1986) and DA6.147 (Guy et al, 1982) were the kind gifts of Dr. M. Nahm (Washington University School of Medicine) and Dr. B. D Schwartz (Monsanto Chem.…”

Section: Cells and Antibodiesmentioning

confidence: 99%

The biogenesis of the MHC class II compartment in human I-cell disease B lymphoblasts.

Glickman

Morton

Slot

et al. 1996

The Journal of Cell Biology

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Abstract. The localization and intracellular transport of major histocompatibility complex (MHC) class II molecules and lysosomal hydrolases were studied in I-Cell Disease (ICD) B lymphoblasts, which possess a mannose 6-phosphate (Man-6-P)-independent targeting pathway for lysosomal enzymes. In the trans-Golgi network (TGN), MHC class II-invariant chain complexes colocalized with the lysosomal hydrolase cathepsin D in buds and vesicles that lacked markers of clathfin-coated vesicle-mediated transport. These vesicles fused with the endocytic pathway leading to the formation of "early" MHC class II-rich compartments (MIICs). Similar structures were observed in the TGN of normal [3 lymphoblasts although they were less abundant. Metabolic labeling and subcellular fractionation experiments indicated that newly synthesized cathepsin D and MHC class II-invariant chain complexes enter a non-clathfin-coated vesicular structure after their passage through the TGN and segregation from the secretory pathway. These vesicles were also devoid of the cation-dependent mannose 6-phosphate (Man-6-P) receptor, a marker of early and late endosomes. These findings suggest that in ICD B lymphoblasts the majority of MHC class II molecules are transported directly from the TGN to "early" MIICs and that acid hydrolases can be incorporated into MIICs simultaneously by a Man-6-P-independent process. M AJOR histocompatibility complex (MHC) 1 molecules mediate the presentation of antigenic peptides by antigen presenting cells (APCs) to T lymphocytes. Almost all cell types express MHC class I molecules, which display a variety of cytoplasmically derived peptides for presentation to CD8-positive T lymphocytes. MHC class II molecules are found on a restricted set of cell types such as B lymphocytes, macrophages, dendritic cells including epidermal Langerhans cells, and thymic epithelial cells. MHC class II molecules bind peptides generated by the proteolysis of exogenous antigens for presentation to CD4-positive T lymphocytes (reviewed in Cresswell, 1994). The MHC class II complex consists of ct and 13 chains that associate in the endoplasmic reticulum (ER) with the 31-33-kD invariant (I) chain (Marks et al., 1990). It is generally accepted that the association of I-chain with MHC class II prevents the binding of endoge-

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Section: Cells and Antibodiesmentioning

confidence: 99%