A new phenanthrene derivative and two diarylheptanoids from the roots of Brassica rapa ssp. campestris inhibit the growth of cancer cell lines and LDL-oxidation

Wu, Qian; Cho, Jin‐Gyeong; Yoo, Ki-Hyun; Jeong, Tae‐Sook; Park, Ji‐Hae; Kim, Su-Yeon; Kang, Ji‐Houn; Chung, In‐Sik; Choi, Myung‐Sook; Lee, Kyung‐Tae; Chung, Hae‐Gon; Bang, Myun‐Ho; Baek, Nam‐In

doi:10.1007/s12272-013-0068-8

Cited by 21 publications

(18 citation statements)

References 13 publications

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“…Materials. BrPa was isolated from B. rapa by a previously described method (15). all cell culture reagents were purchased from Gibco; Thermo Fisher Scientific, inc.. all inhibitors were purchased from calbiochem.…”

Section: Brassicaphenanthrene a From Brassica Rapa Protects Ht22 Neuronal Cells Through The Regulation Of Nrf2-mediated Heme Oxygenase-1 mentioning

confidence: 99%

Brassicaphenanthrene A from Brassica�rapa protects HT22 neuronal cells through the regulation of Nrf2‑mediated heme oxygenase‑1 expression

Lee

Chowdhury

et al. 2019

Mol Med Report

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Brain cell damage that results from oxidative toxicity contributes to neuronal degeneration. The transcription factor nuclear factor-e2-related factor 2 (nrf2) regulates the expression of heme oxygenase (Ho)-1 and glutathione (GSH), and serves a key role in the pathogenesis of neurological diseases. Brassica rapa is a turnip that is unique to Ganghwa county, and is used mainly for making kimchi, a traditional Korean food. in the current study, brassicaphenanthrene a (BrPa) from B. rapa was demonstrated to exhibit protective effects against neurotoxicity induced by glutamate via nrf2-mediated Ho-1 expression. Similarly, BrPa increased the expression of cellular glutathione and glutamine-cysteine ligase genes. Furthermore, BrPa caused the nuclear translocation of nrf2 and increased antioxidant response element (are) promoter activity. nrf2 also mediated Ho-1 induction by BrPa through the Pi3K/akt and JnK regulatory pathways. The results of the present study indicated the neuroprotective effect of BrPa, a natural food component from B. rapa.

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Section: Brassicaphenanthrene a From Brassica Rapa Protects Ht22 Neuronal Cells Through The Regulation Of Nrf2-mediated Heme Oxygenase-1 mentioning

confidence: 99%

Brassicaphenanthrene A from Brassica�rapa protects HT22 neuronal cells through the regulation of Nrf2‑mediated heme oxygenase‑1 expression

Lee

Chowdhury

et al. 2019

Mol Med Report

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“…The results showed that BRARBP extract and identified compounds were not cytotoxic and are in agreement with the previously reported studies of Wu et al (2013) on Brassica rapa spp. Campestris (Wu et al, 2013).…”

Section: Effect Of Brarp Extract On C Elegans Gut Model Against Ementioning

confidence: 99%

Potential application of Brassica rapa subsp. pekinensis extract on fresh beef meat during refrigeration storage

Chelliah

Saravanakumar

Barathikannan

et al. 2019

J Food Process Preserv

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This study dealt about the volatile profile of Brassica rapa subsp. pekinensis (BRARP) using gas chromatography‐mass spectrometry (GC‐MS) and computer‐based molecular docking method. Its antimicrobial effect was also evaluated to determine the bioactive potential of BRARP (1.5% and 2% w/v) extract on the shelf life of beef for 16 days of refrigeration storage. The Caenorhabditis elegans was used as an in vivo model for chemotaxis and colonization in the intestinal lumen of the worm. The 1,2‐benzenedicarboxylic acid was the major bioactive compound revealed by the molecular docking method. The C. elegans model shows that extract was not cytotoxic; however, shows beneficial effect by reducing the E. coli O157 population in the gut of worm. The 2% BRARAP samples maintained significantly lower values (p < .05) for all quality attributes compared to control throughout storage. Thus, 2% of BRARP could be used as an effective natural antimicrobial agent for improving the microbiological safety of beef. Practical applications With the increasing demand for natural ingredients as an alternative to synthetic antimicrobial agents or preservative in food processing, the quest for innovative newer bioactive compounds is increasing. Recently, few researchers have explored the potential of using BRARP, a natural extract as an effective antimicrobial for controlling foodborne pathogens. The results of the present investigation explore the antimicrobial efficacy of BRARP in raw beef meat. The results suggested that BRARP can reduce the meat spoilage losses and maintain the shelf life in all quality attributes, and thus it can be used as a natural antimicrobial agent in the meat industry.

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“…Another investigation showed that 2‐phenylethyl isothiocyanate ( 21 ) and benzyl isothiocyanate ( 36 ) could reduce benzo( a ) pyrene‐induced lung tumorigenesis by the activation of MAP kinases, AP‐1 transcription factors and p53 phosphorylation when conjugated with N ‐acetylcysteine (Lopez‐herrera et al., ). Similar to isothiocyanates, brassicaphenanthrene A ( 110 ), a novel phenanthrene derivative, and two known diarylheptanoids, 6‐paradol ( 108 ) and trans ‐6‐shogaol ( 109 ) showed high inhibition against the growth of human cancer lines (HCT‐116, MCF‐7, and HeLa, IC 50 values ranging from 15.0 to 35.0 μM) and against LDL‐oxidation (IC 50 values ranging from 2.9 to 7.1 μM) (Wu et al., ). A further study on three cancer cell lines (Hep‐2, AMN‐3 and Hela) in vitro revealed that the aqueous extract of turnip showed high inhibition against ANM‐3 (63%) and Hep‐2 (42%) cells at a concentration of 1250 μg/mL, and against Hela cells (64%) at a concentration of 10 mg/mL (Barakat, Obaid, Ali, Hassan, & Abaas, ).…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Phytochemical and Health‐Beneficial Progress of Turnip (Brassica rapa)

Paul

Geng

Yang

et al. 2018

Journal of Food Science

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Despite the advancement of medical science, diseases are part‐and‐parcel of human life. Plants have provided humans with medicines since time immemorial, and are still one of the primary sources for drug discovery. Brassica rapa L., commonly known as turnip, is one of the world's oldest cultivated vegetables. Besides being an important vegetable and source of oil, turnip is also used as a traditional medicine for the treatment of headaches, chest complaints, rheumatisms, oedemas, gonorrhoea, syphilis, and rabies. Glucosinolates and isothiocyanates (mainly 2‐phenylethyl, 4‐pentenyl, and 3‐butenyl derivatives) are the main constituents of turnip with diverse bioactivities, especially for the protective effect against cancers. Besides, flavonoids, phenolics, indoles and volatiles are also concomitant in this plant. Pharmacological investigation on turnip revealed the antitumor, antihypertensive, antidiabetic, antioxidant, antiinflammatory, hepatoprotective, and nephroprotective effects. The anticancer property was found to be the most promising biological activity of turnip with 2‐phenylethyl isothiocyanate, phenylpropionitrile, brassicaphenanthrene A, 6‐paradol, and trans‐6‐shogaol as the major active constituents. Flavonoids and phenolics with high free radical scavenging activity should be corresponding to the antioxidant effects. Arvelexin, an indole derivative in turnip, was reported with various effects involving antiinflamatory, antihypertensive and hypolipidemic potency. In spite of many studies concerning either the chemical constituents or the biological activities of turnip, only a few cases disclosed the active ingredients responsible for diverse bioactivities. This review summarizes the research progress on the chemistry and health‐benefits of turnip over the past 20 years to provide a reference for the further investigation.

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A new phenanthrene derivative and two diarylheptanoids from the roots of Brassica rapa ssp. campestris inhibit the growth of cancer cell lines and LDL-oxidation

Cited by 21 publications

References 13 publications

Brassicaphenanthrene A from Brassica�rapa protects HT22 neuronal cells through the regulation of Nrf2‑mediated heme oxygenase‑1 expression

Brassicaphenanthrene A from Brassica�rapa protects HT22 neuronal cells through the regulation of Nrf2‑mediated heme oxygenase‑1 expression

Potential application of Brassica rapa subsp. pekinensis extract on fresh beef meat during refrigeration storage

Phytochemical and Health‐Beneficial Progress of Turnip (Brassica rapa)

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