A new perfusion culture method with a self-organized capillary network

Sugihara, Kei; Yamaguchi, Yoshimi; Usui, Shiori; Nashimoto, Yuji; Hanada, Sanshiro; Kiyokawa, Etsuko; Yokokawa, Ryuji; Nishiyama, Koichi; Miura, Takashi

doi:10.1371/journal.pone.0240552

Cited by 23 publications

(20 citation statements)

References 34 publications

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“…Using organ setting on a chip, a single gastric organoid has been successfully established ( 56 ). Not singly but in pairs, the use of lumen flow and pressure circulation to induce peristaltic movement showed the feasibility of integrating engineering in organoid culture ( 57 ). Coincidentally, kidney organoids on chips were exposed to shear stress by applying fluid flow, thereby mimicking the kidney environment in vivo ( 58 ).…”

Section: Organoid Culture From Primary Tissuesmentioning

confidence: 99%

Application of Organoids in Carcinogenesis Modeling and Tumor Vaccination

et al. 2022

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Organoids well recapitulate organ-specific functions from their tissue of origin and remain fundamental aspects of organogenesis. Organoids are widely applied in biomedical research, drug discovery, and regenerative medicine. There are various cultivated organoid systems induced by adult stem cells and pluripotent stem cells, or directly derived from primary tissues. Researchers have drawn inspiration by combination of organoid technology and tissue engineering to produce organoids with more physiological relevance and suitable for translational medicine. This review describes the value of applying organoids for tumorigenesis modeling and tumor vaccination. We summarize the application of organoids in tumor precision medicine. Extant challenges that need to be conquered to make this technology be more feasible and precise are discussed.

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Section: Organoid Culture From Primary Tissuesmentioning

confidence: 99%

Application of Organoids in Carcinogenesis Modeling and Tumor Vaccination

et al. 2022

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“…The blood flow is simulated using a pulsatile pump which injects the medium inside the blood vessel and reproduces the rheological features like those observed in vivo . The flow can also be driven by gravity and by capillary effect to eliminate the need for pumps for a more robust and scalable model (Sugihara et al, 2020 ; Yu et al, 2020 ). ECM channels can display an array of blood vessels embedded in a hydrogel, usually type I collagen (Kim et al, 2015 ; Partyka et al, 2017 ; Yu et al, 2020 ).…”

Section: Modeling the Healthy Brain Microvasculaturementioning

confidence: 99%

Three-Dimensional in vitro Models of Healthy and Tumor Brain Microvasculature for Drug and Toxicity Screening

2021

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Tissue vascularization is essential for its oxygenation and the homogenous diffusion of nutrients. Cutting-edge studies are focusing on the vascularization of three-dimensional (3D) in vitro models of human tissues. The reproduction of the brain vasculature is particularly challenging as numerous cell types are involved. Moreover, the blood-brain barrier, which acts as a selective filter between the vascular system and the brain, is a complex structure to replicate. Nevertheless, tremendous advances have been made in recent years, and several works have proposed promising 3D in vitro models of the brain microvasculature. They incorporate cell co-cultures organized in 3D scaffolds, often consisting of components of the native extracellular matrix (ECM), to obtain a micro-environment similar to the in vivo physiological state. These models are particularly useful for studying adverse effects on the healthy brain vasculature. They provide insights into the molecular and cellular events involved in the pathological evolutions of this vasculature, such as those supporting the appearance of brain cancers. Glioblastoma multiform (GBM) is the most common form of brain cancer and one of the most vascularized solid tumors. It is characterized by a high aggressiveness and therapy resistance. Current conventional therapies are unable to prevent the high risk of recurrence of the disease. Most of the new drug candidates fail to pass clinical trials, despite the promising results shown in vitro. The conventional in vitro models are unable to efficiently reproduce the specific features of GBM tumors. Recent studies have indeed suggested a high heterogeneity of the tumor brain vasculature, with the coexistence of intact and leaky regions resulting from the constant remodeling of the ECM by glioma cells. In this review paper, after summarizing the advances in 3D in vitro brain vasculature models, we focus on the latest achievements in vascularized GBM modeling, and the potential applications for both healthy and pathological models as platforms for drug screening and toxicological assays. Particular attention will be paid to discuss the relevance of these models in terms of cell-cell, cell-ECM interactions, vascularization and permeability properties, which are crucial parameters for improving in vitro testing accuracy.

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“…A fundamental problem in tissue engineering is the lack of vasculature within synthetic tissues. Takashi Miura (Kyushu University, Japan) addressed this limitation by developing a simple system for generating a perfusable capillary network in vitro (Sugihara et al, 2020). Finally, Alejandro Aguilera-Castrejon (Hanna group, Weizmann Institute of Science, Rehovot, Israel) described his recent work on advancing the conditions for ex vivo culturing of E5.5 to E11 mouse embryos (Aguilera-Castrejon et al, 2021).…”

Section: Development In a Dishmentioning

confidence: 99%

Engineering life in synthetic systems

Oriola

Spagnoli

2021

Development

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The second EMBO-EMBL Symposium ‘Synthetic Morphogenesis: From Gene Circuits to Tissue Architecture’ was held virtually in March 2021, with participants from all over the world joining from the comfort of their sofas to discuss synthetic morphogenesis at large. Leading scientists from a range of disciplines, including developmental biology, physics, chemistry and computer science, covered a gamut of topics from the principles of cell and tissue organization, patterning and gene regulatory networks, to synthetic approaches for exploring evolutionary and developmental biology principles. Here, we describe some of the high points.

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A new perfusion culture method with a self-organized capillary network

Cited by 23 publications

References 34 publications

Application of Organoids in Carcinogenesis Modeling and Tumor Vaccination

Application of Organoids in Carcinogenesis Modeling and Tumor Vaccination

Three-Dimensional in vitro Models of Healthy and Tumor Brain Microvasculature for Drug and Toxicity Screening

Engineering life in synthetic systems

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