A New Mixed‐Mode Stationary Phase Derived from [2‐(3,4‐epoxycyclohexyl)ethyl]trimethoxysilane as a Coupling Reagent and Its RPLC/HILIC/IEC Applications
Abstract:A new mixed-mode stationary phase derived from [2-(3,4epoxycyclohexyl)ethyl]trimethoxysilane as a coupling reagent was synthesized and evaluated for hydrophilic-interaction/ reversed-phase/ion-exchange HPLC. This phase bearing polar groups as well as nonpolar aliphatic and aromatic groups was synthesized starting from [2-(3,4-epoxycyclohexyl)ethyl]trimethoxysilane and isoleucine amino acid, and it was characterized by elemental analysis, FTIR spectroscopy, SEM, and solid-state 13 C NMR. The new stationary phas… Show more
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