A new metabolic route for the fermentative production of 5-aminovalerate from glucose and alternative carbon sources

Jorge, João M. P.; Pérez-García, Fernando; Wendisch, Volker F.

doi:10.1016/j.biortech.2017.04.108

Cited by 64 publications

(77 citation statements)

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“…Heterologous expression of lysine decarboxylase ( ldcC ), putrescine transaminase ( patA ), and ϒ‐butyraldehyde dehydrogenase ( patD ) from E. coli enabled the production of 5.1 g L −1 5‐aminovalerate in flask culture (Fig. ; Table ) . 5‐Aminovalerate production from alternative carbon sources, such as d ‐xylose and l ‐arabinose, was also demonstrated by additional heterologous expression of xylA from X. campestris and the araBAD operon from E. coli .…”

Section: Production Of Polyamide Monomersmentioning

confidence: 95%

“…; Table ) . 5‐Aminovalerate production from alternative carbon sources, such as d ‐xylose and l ‐arabinose, was also demonstrated by additional heterologous expression of xylA from X. campestris and the araBAD operon from E. coli . Using biosugars from a Miscanthus hydrolysate, 33.93 g L −1 5‐aminovalerate was produced by the fed‐batch cultivation of recombinant C. glutamicum 1857 expressing codon‐optimized davBA genes under a strong H30 promoter (Table ) …”

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

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Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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The fermentative production of platform chemicals in biorefineries is a sustainable alternative to current petroleum‐refining processes. Industrial microorganisms, such as Escherichia coli, Saccharomyces cerevisiae, and Corynebacterium glutamicum, have been engineered as microbial cell factories that are able to utilize biomass for the production of value‐added platform chemicals and polymers. Compared to E. coli and S. cerevisiae, C. glutamicum displays weak carbon catabolite repression and can co‐utilize mixed sugars as carbon sources, without any significant growth retardation. Pathways for the utilization of alternative carbon sources, such as d‐xylose and l‐arabinose from lignocellulosic biomass, lactose and galactose from whey, glycerol from biodiesel, and methanol from natural gas refineries, have been evaluated for chemical production. However, the application of C. glutamicum in biorefineries is limited because it does not secrete hydrolases for the efficient utilization of cellulose, xylan, and starch from lignocellulosic and starch biomass. To solve the limitation, C. glutamicum has been engineered for the consolidated bioprocessing of biomass by the heterologous expression of amylolytic and cellulolytic enzymes. Recently, C. glutamicum has been extensively engineered for polyamide monomer production owing to its ability to produce l‐lysine and l‐glutamate. This review summarizes recent advances in the development of C. glutamicum strains that can utilize renewable biomass resources for the production of industrially important chemicals. It highlights recent progress in metabolic engineering for the production of polyamide monomers. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

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Section: Production Of Polyamide Monomersmentioning

confidence: 95%

Section: Production Of Polyamide Monomersmentioning

confidence: 99%

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Baritugo

Kim

David

et al. 2018

Biofuels Bioprod Bioref

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“…5AVA could be obtained at a higher titer of 63.2 g/L by overexpressing an L-lysine-specific permease (Li et al, 2016). As the transamination activity is limiting (Zhang et al, 2010), the final titer of 6ACA achieved in Turk et al ’s study was only 160 mg/L (Jorge et al, 2017; Turk et al, 2016). In our work, we aimed to develop a strategy to simultaneously produce C5, C6 and C7 NNSCAAs from L-lysine.…”

Section: Resultsmentioning

confidence: 93%

“…The precursors of the two pathways are all derived from the tricarboxylic acid (TCA) cycle which are scarce in cells. With inadequate transamination efficiency previously recognized (Zhang et al, 2010), the final titer achieved by Turk et al was only 160 mg/L (Jorge et al, 2017; Turk et al, 2016).…”

Section: Introductionmentioning

confidence: 91%

“…In addition to its utility in bioplastics, 6ACA was also implicated to promote blood clotting, suggesting potential applications as an antifibrinolytic agent (Lu et al, 2015; Schou-Pedersen et al, 2015). Whereas 5AVA biosynthesis is a viable approach for industrial production, effective methods to biosynthesize other NNSCAAs at scale has yet to be established (Jorge et al, 2017; Turk et al, 2016). Biosynthesis of 6ACA was first demonstrated to occur through the condensation of acetyl-CoA and succinyl-CoA (Turk et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Production of nonnatural straight-chain amino acid 6-aminocaproate via an artificial iterative carbon-chain-extension cycle

Cheng

Song²,

Wang³

et al. 2019

Preprint

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22Bioplastics produced from microbial source are promising green alternatives to 23 traditional petrochemical-derived plastics. Nonnatural straight-chain amino acids, 24 especially 5-aminovalerate, 6-aminocaproate and 7-aminoheptanoate are potential 25 monomers for the synthesis of polymeric bioplastics as their primary amine and 26 carboxylic acid are ideal functional groups for polymerization. Previous pathways for 27 5-aminovalerate and 6-aminocaproate biosynthesis in microorganisms are derived from 28 L-lysine catabolism and citric acid cycle, respectively. Here, we show the construction 29 of an artificial iterative carbon-chain-extension cycle in Escherichia coli for 30 simultaneous production of a series of nonnatural amino acids with varying chain length. 31 Overexpression of L-lysine α-oxidase in E. coli yields 2-keto-6-aminocaproate as a 32 non-native substrate for the artificial iterative carbon-chain-extension cycle. The chain-33 extended α-ketoacid is subsequently decarboxylated and oxidized by an α-ketoacid 34 decarboxylase and an aldehyde dehydrogenase, respectively, to yield the nonnatural 35 straight-chain amino acid products. The engineered system demonstrated simultaneous 36 in vitro production of 99.16 mg/L of 5-aminovalerate, 46.96 mg/L of 6-aminocaproate 37 and 4.78 mg/L of 7-aminoheptanoate after 8 hours of enzyme catalysis starting from 2-38 keto-6-aminocaproate as the substrate. Furthermore, simultaneous production of 2.15 39 g/L of 5-aminovalerate, 24.12 mg/L of 6-aminocaproate and 4.74 mg/L of 7-40 aminoheptanoate was achieved in engineered E. coli. This work illustrates a promising 41 metabolic-engineering strategy to access other medium-chain organic acids with -NH2, 42 -SCH3, -SOCH3, -SH, -COOH, -COH, or -OH functional groups through carbon-chain-43 elongation chemistry.44 Keywords: Nonnatural straight chain amino acid, 6-Aminocaproate, Carbon chain 45 elongation, Synthetic biology, Iterative cycle 46 3 47 Abbreviations: 48 NNSCAA, Nonnatural straight chain amino acid; 5AVA, 5-Aminovalerate; 6ACA, 6-49

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Metabolic Engineering of Corynebacterium glutamicum

Becker

Wittmann

2021

Metabolic Engineering

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A new metabolic route for the fermentative production of 5-aminovalerate from glucose and alternative carbon sources

Cited by 64 publications

References 50 publications

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Recent advances in metabolic engineering of Corynebacterium glutamicum as a potential platform microorganism for biorefinery

Production of nonnatural straight-chain amino acid 6-aminocaproate via an artificial iterative carbon-chain-extension cycle

Metabolic Engineering of Corynebacterium glutamicum

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