A New Galactose-Specific Lectin from Clerodendrum infortunatum

Abstract

Background The ethno-medical significance of Clerodendrum genus raises the interest towards the characterization of its seed lectin by inexpensive and most effective technique. Objective The focus of this study is the purification, characterization, and evaluation of the antioxidant and antiproliferative potential of a galactose-specific lectin from Clerodendrum infortunatum L. seeds. Materials and Methods The crude extract, homogenized in 6 volumes of the saline containing 10 mM β-mercaptoethanol was subjected to pigment removal by Toyopeal HW-55 column prior to ammonium sulfate fractionation (40-80 %). The crude protein extract was then loaded to the gel filtration column Sephadex G-200 followed by affinity chromatography using activated galactose coupled Sepharose-4B. Results The SDS-PAGE analysis showed a single band of about 30 kDa which further determined by MALDI-TOF analysis. The MALDI-TOF spectra revealed that Clerodendrum infortunatum lectin (CIL) is a homo-tetramer of 120 kDa consisting of four identical subunits of 30 kDa. The haemagglutination inhibition assay was done with purified lectin by many sugars, among which N-acetyl-D-galactosmine (NAG), D-galactose and lactose exhibited high inhibition. NAG showed the highest inhibition amongst the tested sugars, having the minimum inhibitory concentration of about 0.97 mM. The lectin exhibited a moderate antioxidant activity with an IC 50 value of 6.1 ± 0.1 mg.mL -1 and induced cell death with IC 50 of 82.8 μg.mL -1 against human gastric cancer cell line, AGS, indicated the potential of CIL for clinical and therapeutic applications. Conclusion The present study demonstrated the moderate ability of the CIL to inhibit the growth of human gastric cancer cells, AGS either by causing cytotoxic or anti-proliferative effects. Thus, CIL due to its remarkable properties may be considered as a potential bio-molecule in tumor research and glycobiology.

A new lectin from Crotalaria incana seeds and studies of toxicity in Artemia salina nauplii

“…Based on the activity of CiL at different pH, it was possible to verify optimum activity at acidic and neutral pH, results similar to those of other reports on lectins of the Fabaceae family, such as the lectins of Dioclea reflexa (Pinto-Junior et al 2016), Canavalia villosa (Lossio et al 2017) and Parkia panurensis (Cavada et al 2019). Other studies report lectins from other botanical families with maximum activity in more acidic and basic regions, e.g., Clerodendrum infortunatum from the Lamiaceae family with optimum activity at pH 2.0 (Surya & Haridas 2018) and Litchi chinensis from the Sapindaceae family with optimum activity at pH 8 and 9 (Bose et al 2016).…”

Polyphenol Mediated Suppression of Hepatocellular Carcinoma (HepG2) Cell Proliferation by Clerodendrum infortunatum L. Root

“…Clerodendrum L. exerts selective cytotoxic activity with phenolic and flavonoids present in the plant extract (leaves, seeds) where they interfere with multiple signal transduction pathways in cells such as TRAIL-induced cytotoxicity, increased expression of p53 and p21, and by increased concentration of cytochrome c [ 53 , 62 ]. Therefore, we investigated dose dependent HepG2 cell growth inhibition using AECIR after removing polyphenols from the AECIR.…”