A new endoplasmic reticulum-targeted two-photon fluorescent probe for imaging of superoxide anion in diabetic mice

Xiao, Haibin; Xiao, Liu; Wu, Chuanchen; Wu, Yaohuan; Li, Ping; Guo, Xiaomeng; Tang, Bo

doi:10.1016/j.bios.2016.12.068

Cited by 97 publications

(45 citation statements)

References 69 publications

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“…Indeed, as also reported by two other groups that modified hydrocyanines for imaging purposes (derivatizing HCy5 with PEG or coupling them to a nano‐carrier), the detected response towards superoxide and hydroxyl radicals was lower compared to the original report . All these findings suggest that coupling the (hydro)cyanines to a macromolecule may influence the fluorescence properties and/or sensing ability and may account for the reduced sensing capacity and a high LOD (3.5 and 15.3 μ m ) compared to other examples in literature (80–90 n m ) . However, cyanines are also known to form H‐aggregates in water and buffer especially at higher concentrations, which may also occur with hydrocyanines and alter their sensing behavior .…”

Section: Figuresupporting

confidence: 48%

See 1 more Smart Citation

Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide

Andina

Munzinger

Frei

et al. 2017

Chemistry A European J

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Extracellular detection of endogeneous analytes (e.g., superoxide) can provide important insights into mechanisms of homeostasis and diseases, such as tumorigenesis. A ratiometric probe with a fluorescent reference and an analyte-specific switch-on dye was developed. Detection of ROS in the extracellular milieu was ensured by connecting the two fluorophores with a modular peptide-nucleic-acid-based linker. The ROS-sensing ability was assessed and validated in cell-free assays and in cell culture.

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Section: Figuresupporting

confidence: 48%

“…Although selective superoxide detection has been improved recently, the sensing unit used in ratiometric probe designs is mostly hydroethidin (HE) . The reliability of this fluorophore indeed is plagued by several side reactions, for example, with cytochrome c .…”

Section: Figurementioning

confidence: 99%

Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide

Andina

Munzinger

Frei

et al. 2017

Chemistry A European J

View full text Add to dashboard Cite

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“…In addition, two‐photon fluorescence probes for O 2 •− have been developed. Tang and co‐workers synthesized a two‐photon fluorescent probe based on the fluorophore naphthalimide. The probe was designed for the detection of the O 2 •− in the endoplasmic reticulum (ER) of living cells through functionalization with a group that enables targeting of the ER.…”

Section: Fluorescent Probes For the Detection Of Ros‐related Diseasesmentioning

confidence: 99%

“…The fluorescence images in liver tissues of normal mice (Normal), STZ diabetic mice (STZ), STZ mice with tiron saline solution injection (STZ+Tiron), and STZ mice after oral gavage of metformin (STZ+Metf) stained with ER‐BZT (right). Reproduced with permission . Copyright 2016, Elsevier B.V.…”

Section: Fluorescent Probes For the Detection Of Ros‐related Diseasesmentioning

confidence: 99%

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

et al. 2019

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Reactive oxygen species (ROS) consist of a diverse range of oxidative small molecular ions and free radicals that are produced throughout the body during certain biological processes. Due to their high reactivity, these molecules result in the damage of tissues and cells. Therefore, ROS have been implicated in an array of diseases including cancer, inflammation, and neurodegenerative diseases such as Parkinson's disease and Alzheimer's disease. Owing to the simplicity, sensitivity, and selectivity of fluorescence‐based strategies, many small‐molecular sensors or imaging agents have been developed to sense and visualize ROS both in vitro and in vivo. Likewise, activatable drug delivery and prodrug systems that can be triggered by ROS for disease theranostics (diagnostic and therapeutic combined) have been developed. Herein, recently developed fluorescence‐based sensing and imaging agents for the detection of ROS both intracellularly and in vivo are summarized. In addition, drug delivery, which require activation by ROS to achieve disease theranostics is also discussed.

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“…[4] However, organelles are scattered or dispersed in cells in am anner that renders selective irradiation of ac omplete organelle (e.g., mitochondria or golgi apparatus) impractical, undermining the utility of the strategy in this context. Such motifs include,f or example,t riphenylphosphonium [5] (TPP), phenyl sulfonamide, [6] and tertiary/secondary amines [7] that tend to accumulate in mitochondria, endoplasmic reticulum (ER), and lysosomes/endosomes,respectively.Their coupling to small-and even macro-molecules was found to effectively impart sub-cellular specificity to the entire conjugate. Such motifs include,f or example,t riphenylphosphonium [5] (TPP), phenyl sulfonamide, [6] and tertiary/secondary amines [7] that tend to accumulate in mitochondria, endoplasmic reticulum (ER), and lysosomes/endosomes,respectively.Their coupling to small-and even macro-molecules was found to effectively impart sub-cellular specificity to the entire conjugate.…”

mentioning

confidence: 99%

Organelle‐Targeted BODIPY Photocages: Visible‐Light‐Mediated Subcellular Photorelease

et al. 2019

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Photocaging facilitates non-invasive and precise spatio-temporal control over the release of biologically relevant small-and macro-molecules using light. However,s ubcellular organelles are dispersed in cells in am anner that renders selective light-irradiation of ac omplete organelle impractical. Organelle-specific photocages could provide ap owerfulm ethod for releasing bioactive molecules in subcellular locations.H erein, we report ag eneral post-synthetic method for the chemical functionalization and further conjugation of meso-methyl BODIPY photocages and the synthesis of endoplasmic reticulum (ER)-, lysosome-, and mitochondria-targeted derivatives.W ea lso demonstrate that 2,4dinitrophenol, am itochondrial uncoupler,a nd puromycin, ap rotein biosynthesis inhibitor,c an be selectively photoreleased in mitochondria and ER, respectively,i nl ive cells by using visible light. Additionally,p hotocaging is shown to lead to higher efficacy of the released molecules,probably owingto alocalized and abrupt release.Supportinginformation and the ORCID identification number(s) for the author(s) of this article can be found under: https://doi.org/10.

show abstract

A new endoplasmic reticulum-targeted two-photon fluorescent probe for imaging of superoxide anion in diabetic mice

Cited by 97 publications

References 69 publications

Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide

Development of a Modular Ratiometric Fluorescent Probe for the Detection of Extracellular Superoxide

Sensors, Imaging Agents, and Theranostics to Help Understand and Treat Reactive Oxygen Species Related Diseases

Organelle‐Targeted BODIPY Photocages: Visible‐Light‐Mediated Subcellular Photorelease

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