A new approach to the construction of potentiometric immunosensors

Ghindilis, Andrey L.; Skorobogat'ko, O. V.; Gavrilova, V. P.; Yaropolov, A. I.

doi:10.1016/0956-5663(92)87008-d

Cited by 38 publications

(19 citation statements)

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“…7,36,37 An example is the use of laccase (enzyme label) for oxygen measurements. 38 The immobilization of intact microorganisms on the surface of an ISE has also been described in the literature. 7,36,37 In addition, potentiometric immunosensors have been developed, which are based on the change in potential when either an antibody or an antigen is bound to its specific partner that has been immobilized on the electrode.…”

Section: Potentiometric Sensorsmentioning

confidence: 99%

“…7,36,37 In addition, potentiometric immunosensors have been developed, which are based on the change in potential when either an antibody or an antigen is bound to its specific partner that has been immobilized on the electrode. 38 Recent advances can be illustrated by the use of carbon working and silver reference electrodes, supported in ceramic plates, for the analysis of human serum albumin (HSA). 39 The anti-HSA IgG is adsorbed onto the surface of a thick-film electrode.…”

Section: Potentiometric Sensorsmentioning

confidence: 99%

See 1 more Smart Citation

Electrochemical sensors: a powerful tool in analytical chemistry

Stradiotto¹,

Yamanaka²,

Zanoni³

2003

J. Braz. Chem. Soc.

327

154

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Sensores potenciométricos, amperométricos e condutométricos são sensores eletroquímicos com imensa aplicação nas áreas de meio ambiente, indústria e análises clínicas. O presente trabalho apresenta uma revisão generalizada dos aspectos fundamentais, os desenvolvimento e a contribuição destes sensores na área de química analítica, e reporta alguns aspectos relevantes dos avanços na área de sensores eletroquímicos no Brasil.Potentiometric, amperometric and conductometric electrochemical sensors have found a number of interesting applications in the areas of environmental, industrial, and clinical analyses. This review presents a general overview of the three main types of electrochemical sensors, describing fundamental aspects, developments and their contribution to the area of analytical chemistry, relating relevant aspects of the development of electrochemical sensors in Brazil.

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Section: Potentiometric Sensorsmentioning

confidence: 99%

Section: Potentiometric Sensorsmentioning

confidence: 99%

Electrochemical sensors: a powerful tool in analytical chemistry

Stradiotto¹,

Yamanaka²,

Zanoni³

2003

J. Braz. Chem. Soc.

327

154

View full text Add to dashboard Cite

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“…The decrease in rate is proportional to free antigen concentration in the solution. Ghindilis [37,38] described potentiometric immunoelectrodes with laccase as the enzyme-label. The enzyme laccase catalyses a reaction of oxygen electroreduction by a mediatorless mechanism.…”

Section: Potentiometric Biosensorsmentioning

confidence: 99%

Electrochemical Biosensors for Detection of Biological Warfare Agents

Shah

Wilkins

2003

Electroanalysis

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This review discusses current development in electrochemical biosensors for detection of biological warfare agents. This could include bacteria, viruses and toxins that are aerosoled deliberately in air, food or water to spread terrorism and cause disease or death to humans, animals or plants. The rapid and unequivocal detection and identification of biological warfare agents is a major challenge for any government including military, health and other government agents. Reliable, specific characterization and identification of the microorganism from sampling location, either air, water, soil or others is required. This review will survey different types of electrochemical biosensors has been developed based on the following: i) Immunosensors ii) PCR (DNA base Sensor) iii) Bacteria or whole cell sensor and iv) Enzyme sensor. This article gives an overview of electrochemical biosensor for detection of biological warfare agents. Electrochemical biosensors have the advantages of sensitivity, selectivity, to operate in turbid media, and amenable to miniaturization. Recent developments in immunofiltration, flow injection, and flow-through electrochemical biosensors for bacteria, viruses, and toxin detection are reviewed. The current research and development in biosensors for biological warfare agents detection is of interest to the public as well as to the defense is also discussed.

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“…As supporting electrolyte, 0.1 M phosphate buffer at pH 7 [29] was used when studying tree laccase, and 0.1 M acetate buffer at pH À 5 [3,5] was used when studying the fungal laccase.…”

Section: Instrumentationmentioning

confidence: 99%

“…Laccase (EC 1.10.3.2) is a member of the blue multi-copper oxidase family [1], which catalyzes the oxidation of a variety of organic substrates coupled to the reduction of molecular oxygen to water [2,3]. The most common uses of laccase are in the pulp and paper industries, wastewater treatment and textile industries [4].…”

Section: Introductionmentioning

confidence: 99%

Laccase Biosensor on Monolayer‐Modified Gold Electrode

Gupta¹,

Rajendran²,

Atanassov³

2003

Electroanalysis

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Laccase enzymes from two different sources, namely, tree laccase from Rhus vernicifera and fungal laccase from Coriolus hirsutus were used for the development of biosensor for catechol. Laccase was immobilized onto the amine terminated thiol monolayers on gold surface by glutaraldehyde coupling. From the different thiol monolayers investigated, cystamine was found to be optimal with respect to sensitivity, stability, reproducibility, and other electrochemical properties of the enzyme electrode. Linear calibration in the range between 1 and 400 mM for catechol was obtained for fungal laccase covalently coupled on the electrode surface. The kinetic parameters determined using the Lineweaver-Burk and Eadie-Hofstee plots were K m 0.65 mM and V max 24.5 mA for fungal laccase compared to K m 5.4 mM and V max 6.6 mA for tree laccase on cystamine monolayer. The electrode showed good stability for 1 month without loosing appreciable activity when stored dry in a refrigerator at À 20 8C.

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A new approach to the construction of potentiometric immunosensors

Cited by 38 publications

References 1 publication

Electrochemical sensors: a powerful tool in analytical chemistry

Electrochemical sensors: a powerful tool in analytical chemistry

Electrochemical Biosensors for Detection of Biological Warfare Agents

Laccase Biosensor on Monolayer‐Modified Gold Electrode

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