A new approach to predict human intestinal absorption using porcine intestinal tissue and biorelevant matrices

Westerhout, Joost; Steeg, Evita van de; Grossouw, Dimitri; Zeijdner, Evelijn; Krul, Cyrille; Verwei, Miriam; Wortelboer, Heleen M.

doi:10.1016/j.ejps.2014.07.003

Cited by 111 publications

(103 citation statements)

References 38 publications

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“…13,14 Porcine intestines (ileum) provided by a local slaughterhouse were used as permeability barrier membrane for this study. The intestines were sectioned and washed with PBS to remove the lumen contents prior to the absorption study.…”

Section: In Vitro Intestinal Absorption Studymentioning

confidence: 99%

Development of a novel microemulsion for oral absorption enhancement of all-trans retinoic acid

Subongkot¹,

Ngawhirunpat²

2017

IJN

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This study was aimed to develop a novel microemulsion that contained oleth-5 as a surfactant to enhance the oral absorption of all-trans retinoic acid (ATRA). The prepared microemulsion was evaluated for its particle size, shape, zeta potential, in vitro release, in vitro intestinal absorption, intestinal membrane cytotoxicity and stability. The obtained microemulsion was spherical in shape with a particle size of <200 nm and a negative surface charge. The in vitro release of the ATRA-loaded microemulsion was best fit with the zero-order model. This microemulsion significantly improved the intestinal absorption of ATRA. Confocal laser scanning microscopy analysis using a fluorescent dye-loaded microemulsion also confirmed the intestinal absorption result. The intestinal membrane cytotoxicity of the ATRA-loaded microemulsion did not differ from an edible oil (fish oil). Stability testing showed that the ATRA-loaded microemulsion was more stable at 25°C than 40°C.

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Section: In Vitro Intestinal Absorption Studymentioning

confidence: 99%

Development of a novel microemulsion for oral absorption enhancement of all-trans retinoic acid

Subongkot¹,

Ngawhirunpat²

2017

IJN

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“…When diluting compounds in the KRB buffer, care should be taken that the levels of alcohol and DMSO are below 1 %. It has been shown that the use of undiluted digested food samples from the TIM model (including bile, pancreatic enzymes and/or food components) can be applied onto the apical side of the tissue segments if mounted in a chamber (Westerhout et al 2014 ). However, the use of undiluted digested food samples is not yet tested for the intestinal segment system.…”

Section: Incubation With Food Components and Digested Food Samplesmentioning

confidence: 99%

Porcine Ex Vivo Intestinal Segment Model

Ripken

Hendriks

2015

The Impact of Food Bioactives on Health

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This chapter describes the use of the porcine ex vivo intestinal segment model. This includes the advantages and disadvantages of the segment model and a detailed description of the isolation and culture as well as the applications of the porcine ex vivo intestinal segment model in practice. Compared to the Ussing chamber (Chap. 24 ) the porcine ex vivo small intestinal segment model is a relatively simple to use intestinal tissue model. The main difference being that the tissue segment is not mounted in a chamber, but is freely fl oating in a solution. Therefore the ex vivo intestinal segment model does not distinguish between the apical and basolateral side of the tissue. The intestinal segments can be obtained from various anatomical regions of the small intestine (e.g. duodenum, jejunum, ileum or even the colon) and the segments consist of various cell types (e.g. epithelial cells, paneth cells, goblet cells, enterochromaffi n cells and enteroendocrine cells). The intestinal segment model has been shown to be a suitable tool to study compound and location specifi c effects on the release of gastrointestinal hormones and gastrointestinal metabolism of endocannabinoids and related compounds.

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“…In a previous study we have shown that the intestinal absorption of chemicals from undiluted digestion matrix can be studied by using ex vivo intestinal tissue segments from pigs [27]. Since pigs also have an omnivorous diet, their GI tract is very much comparable to that of humans [6,[28][29][30][31].…”

Section: Introductionmentioning

confidence: 99%

Prediction of Oral Absorption of Nanoparticles from Biorelevant Matrices Using a Combination of Physiologically Relevant In Vitro and Ex Vivo Models

Westerhout¹,

Bellmann²,

Ee³

et al. 2017

J Food Chem Nanotechol

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The application of nanoparticles (NPs) in food products is ever increasing. For consumer safety it is essential to know the human oral bioavailability of these NPs. In the present study, a combination of physiologically relevant in vitro and ex vivo models are presented, which combined are considered to predict human intestinal digestion and absorption of NPs more accurately than commonly applied animal or cellular studies.First, a computer controlled dynamic in vitro gastrointestinal model (tiny-TIM) is used for simulation of the digestive processes upon gastro-intestinal passage of non-biodegradable aminated, carboxylated, or unmodified fluorescent polystyrene NPs (PS-NPs). Then, the pretreated (digested) PS-NPs were consequently evaluated for absorption using ex vivo porcine intestinal tissue.In this study, we found that the digestion of non-biodegradable aminated, carboxylated, or unmodified fluorescent polystyrene NPs (PS-NPs) leads to different bioaccessible concentrations, with unmodified PS-NP concentrations being 3.5-and 4.5-fold lower than the aminated and carboxylated PS-NPs, respectively. In addition, we observed a reduced absorption of PS-NPs by ex vivo porcine intestinal tissue from the digestive matrix when compared to the absorption of their undigested counterparts in Krebs-Ringer Bicarbonate (KRB) buffer. The current results prove that oral absorption of PS-NPs in humans is to be expected in vivo. In the risk assessment of potential oral exposure to NPs in food products it is therefore key that the exposure and hazard assessment are performed with the NPs in physiologically relevant conditions, since this may influence the absorption and hazard properties of the NPs.

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A new approach to predict human intestinal absorption using porcine intestinal tissue and biorelevant matrices

Cited by 111 publications

References 38 publications

Development of a novel microemulsion for oral absorption enhancement of all-trans retinoic acid

Development of a novel microemulsion for oral absorption enhancement of all-trans retinoic acid

Porcine Ex Vivo Intestinal Segment Model

Prediction of Oral Absorption of Nanoparticles from Biorelevant Matrices Using a Combination of Physiologically Relevant In Vitro and Ex Vivo Models

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