A new and efficient micropropagation method and its breeding applications in Asparagus genera

Martín, E.; Regalado, J. J.; Padilla, Isabel; Westendorp, N.; Encina, Carlos López

doi:10.1007/s11240-014-0548-7

Cited by 13 publications

(15 citation statements)

References 33 publications

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“…Snowcap. We had reached 100% of rooted explants (small and big size explants maintained in MSM-1), incubating these explants during 4-5 weeks in MSM-3, a medium supplemented with a low level of sucrose (1%) plus 5 mg l − 1 ancymidol, in agreement with positive effect on rooting induction obtained in other species such in Pinus strobus( Burkhart and Meyer, 1991) or several species of Asparagus(Desjardins et al, 1987, Stajner et al, 2002, Carmona et al, 2014, Regalado et al, 2015, this growth retardant, an inhibitor of gibberellin biosynthesis, is able to reduce the growth of the aerial part of the plants and promote rooting in Snowcap plants, developing a good root system in each explant (Table…”

supporting

confidence: 71%

In Vitro Mass Micropropagation of Mammillaria Vetula ssp. Gracilis var. Arizonica Snowcap

Lopez-Granero

Arana

González

et al. 2021

Preprint

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A mass micropropagation method was developed for Mammillaria vetula ssp. gracilis cv. Arizonica Snowcap, a cactus species with high ornamental value. A culture media based in MS medium (Murashige and Skoog, 1962) supplemented with 0.1 mg l− 1 NAA + 0.3 mg l− 1 KIN + 5.0 mg l− 1 GA3 + 30 g l− 1 sucrose (MSM-1), was developed specifically for the establishment and multiplication of Mammillaria vetula cv. Snowcap offshoots, let us to produce a proliferation rate of 34.9 ± 5.9 new offshoots per explant. The non-rooted offshoots were rooted in MSM-2, consisting in MS salts supplemented with 0.1 mg l− 1 NAA + 0.3 mg l− 1 KIN plus 5 mg l− 1 ancymidol and a lower dose (10 g l− 1) of sucrose, with a rooting rate of 100%. The rate of acclimatization of the rooted offshoots was 100%. The full process of micropropagation from aseptic establishment in vitro to the end of plant acclimation takes approximately between 5 and 6 months and the plant production can be maintained continuously during all year.

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supporting

confidence: 71%

In Vitro Mass Micropropagation of Mammillaria Vetula ssp. Gracilis var. Arizonica Snowcap

Lopez-Granero

Arana

González

et al. 2021

Preprint

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“…However, a general protocol has not been reported that is valid for all the species of Asparagus and interspecific hybrids between them. The only method that has been used for the micropropagation of multiple species is the protocol developed by Carmona-Martin et al (2014). This procedure was developed for micropropagation of the Spanish landrace ''Morado de Hue ´tor'' (2n = 4x = 40).…”

Section: Introductionmentioning

confidence: 99%

Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

Regalado

Martín

Castro

et al. 2015

Plant Cell Tiss Organ Cult

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An efficient micropropagation method for asparagus species was developed in this study. The method allows the fast cloning of the elite genotypes from different asparagus species and the interspecific hybrids obtained from these species. Rhizome bud explants were disinfected using 3 g l -1 of benomyl and 20 g l -1 of sodium hypochlorite. Then, they were cultured on Asparagus Rhizome Bud Medium 3 (ARBM-3) consisting in modified Murashige and Skoog medium with salts with EDDHA-Fe (85.7 mg l -1 ) instead of EDTA-Fe and vitamins, supplemented with 0.3 mg l -1 NAA, 0.1 mg l -1 KIN, 2 mg l -1 ancymidol and 6 % sucrose. Results showed that the method developed produced high disinfection rates (70-95 %). More than 70 % of the explants developed shoots and the rooting rate on ARBM-3 medium was 30-45 %. The rooting rate increased to 60-85 % when the unrooted shoots were subjected to an additional cycle of rooting, reaching 100 % after two cycles of rooting. The multiplication was achieved through mechanical division of rooted shoot clusters growing in ARBM-3. The acclimatization rate of the micropropagated plantlets was higher than 90 %. The micropopagated plantlets were screened for somaclonal variation using 12 expressed sequence tag derived simple sequence repeat markers. The results confirmed the character ''true to type'' of the plantlets, indicating that the method developed in this study can be used to successfully micropropagate asparagus species.

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“…So, the use of the PM5 and PM6 in the anther culture supposes an important advantage in plantlet regeneration, shortening the time needed and the number of subcultures, reducing the possibilities of somaclonal variation (Bairu et al 2011). The rooting of the unrooted shoots in the PMs media, the multiplication of the PRACs and the acclimatization of plantlets reach high success rates following the methods previously developed for our group (Carmona-Martin et al 2014;Regalado et al 2015a).…”

Section: Discussionmentioning

confidence: 96%

“…The same plantlets characterized with the sex-linked marker Asp1-T7 were characterized with these EST-SSRs. PCR conditions used to carry out this characterization were described by Carmona-Martin et al (2014).…”

Section: Characterization With Est-ssrs Of the Plantlets Regenerated mentioning

confidence: 99%

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

Regalado

Martín

Madrid

et al. 2015

Plant Cell Tiss Organ Cult

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Anther culture is used to develop asparagus ''super-male'' (di-haploids) in asparagus, which can be used to develop ''all-male'' varieties, by crossing them with suitable females; their progenies will be formed only by males which is advantageous for producers. This report describe a new anther culture protocol adapted to ''Morado de Huétor'', a Spanish tetraploid landrace, and studied the different factors involved in callus proliferation success from anther explants such as the microspore development stage, or the type of stress used to induce the symmetric division of the microspores, to obtain a high success rate (90 %). For plantlets regenerates from anther culture (PRACs) regeneration we develop a proliferation media supplemented with a combination of pCPA and BA able to induce callus proliferation and plantlet regeneration in the same step in a 50 % of calli, simplifying the procedure. The high percentage of heterozygous male recovery, originated from somatic cells, is an important problem in the anther culture, and to elucidate the origin of PRACs we have combined different tools: ploidy analysis, characterization with the linked sex-marker Asp1-T7 and with ESTSRRs. We can establish that 50 % of PRACs obtained in this work were regenerated from diploid microspores of ''Morado de Huétor'', regenerating diploid, di-diploid and tetra-diploid plantlets. The di-diploids males (MMmm) would generate a ratio male:female of 5:1 (83.3 %) and the tetra-diploid males (MMMMmmmm) a ratio male:female of 69:1 (98.6 %), so the tetra-diploid males could be considered ''super-males'' and be used to develop ''all-male'' varieties of ''Morado de Huétor''.

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A new and efficient micropropagation method and its breeding applications in Asparagus genera

Cited by 13 publications

References 33 publications

In Vitro Mass Micropropagation of Mammillaria Vetula ssp. Gracilis var. Arizonica Snowcap

In Vitro Mass Micropropagation of Mammillaria Vetula ssp. Gracilis var. Arizonica Snowcap

Micropropagation of wild species of the genus Asparagus L. and their interspecific hybrids with cultivated A. officinalis L., and verification of genetic stability using EST-SSRs

Production of “super-males” of asparagus by anther culture and its detection with SSR-ESTs

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