A neuroprotective role of glial cell line-derived neurotrophic factor following moderate spinal cord contusion injury

Iannotti, Christopher A.; Zhang, Y. Ping; Shields, Christopher B.; Han, Yingchun; Burke, Darlene A.; Xu, Xiao–Ming

doi:10.1016/j.expneurol.2004.05.033

Cited by 90 publications

(43 citation statements)

References 63 publications

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“…In addition, the upregulation of GFRα1 and c-Ret gene expression, two GDNF receptors, following SCI may further increase the responsiveness of injured neurons and axons to GDNF (Widenfalk et al, 2001). Iannotti et al demonstrated that long-term GDNF intrathecal infusion elicited neuroprotective effects on contused adult rat spinal cords, resulting in a substantial degree of tissue and axonal sparing (Iannotti et al, 2004). GDNF mRNA expression is highly upregulated 8 hours post-SCI and downregulated from 12 hours to 12 weeks post-SCI (Gerin et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Ansorena

Berdt

Ucakar

et al. 2013

International Journal of Pharmaceutics

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We hypothesized that local delivery of GDNF in spinal cord lesion via an injectable alginate hydrogel gelifying in situ would support spinal cord plasticity and functional recovery. The GDNF release from the hydrogel was slowed by GDNF encapsulation in microspheres compared to non-formulated GDNF (free GDNF). When injected in a rat spinal cord hemisection model, more neurofilaments were observed in the lesion when the rats were treated with free GDNF-loaded hydrogels. More growing neurites were detected in the tissues surrounding the lesion when the animals were treated with GDNF microsphere-loaded hydrogels. Intense GFAP (astrocytes), low βIII tubulin (neural cells) and RECA-1 (endothelial cells) stainings were observed for non-treated lesions while GDNF-treated spinal cords presented less GFAP staining and more endothelial and nerve fiber infiltration in the lesion site. The animals treated with free GDNF-loaded hydrogel presented superior functional recovery compared with the animals treated with the GDNF microsphere-loaded hydrogels and non-treated animals.

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Section: Introductionmentioning

confidence: 99%

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Ansorena

Berdt

Ucakar

et al. 2013

International Journal of Pharmaceutics

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“…23 Cross-sections of the injured spinal cord were dehydrated with 70% and 95% alcohol for 2 min each and then were immersed with 0.1% LFB solution at 37°C for 4 h. After cooling at 4°C for 20 min, the slides were dipped in 95% alcohol 5 times and dH 2 O for 1 min and then were cleared and cover-slipped.…”

Section: Resultsmentioning

confidence: 99%

Assessment of White Matter Loss Using Bond-Selective Photoacoustic Imaging in a Rat Model of Contusive Spinal Cord Injury

Wang

Cheng

et al. 2014

Journal of Neurotrauma

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White matter (WM) loss is a critical event after spinal cord injury (SCI). Conventionally, such loss has been measured with histological and histochemical approaches, although the procedures are complex and may cause artifact. Recently, coherent Raman microscopy has been proven to be an emerging technology to study de-and remyelination of the injured spinal cord; however, limited penetration depth and small imaging field prevent it from comprehensive assessments of large areas of damaged tissues. Here, we report the use of bond-selective photoacoustic (PA) imaging with 1730-nm excitation, where the first overtone vibration of CH 2 bond is located, to assess WM loss after a contusive SCI in adult rats. By employing the first overtone vibration of CH 2 bond as the contrast, the mapping of the WM in an intact spinal cord was achieved in a label-free three-dimensional manner, and the physiological change of the spinal cord before and after injury was observed. Moreover, the recovery of the spinal cord from contusive injury with the treatment of a neuroprotective nanomedicine ferulic-acid-conjugated glycol chitosan (FA-GC) was also observed. Our study suggests that bond-selective PA imaging is a valuable tool to assess the progression of WM pathology after SCI as well as neuroprotective therapeutics in a label-free manner.

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“…Neurotrophic growth factors are small molecules NT-3/NT-4 Brain-derived neurotrophic factor BDNF Fibroblast growth factor FGF Glia cell line derived neurotrophic factor GDNF with a wide range of activities, including the promotion of neuron survival and axonal outgrowth, as well as the regulation of neuron-target interactions (Table 5; 15, 16). The most interesting activities of these factors in the context of spinal cord injury seem to be their ability to stimulate survival of injured neurons, as well as to regulate the expression of RAGs in a way that increase the regeneration capacity of injured axon (17)(18)(19)(20). Although several growth factors have well documented effects on axonal growth, there are problems with their application in spinal cord injury.…”

Section: Promoting the Intrinsic Neuron Capacity For Regenerationmentioning

confidence: 99%

Spinal cord injury – scientific challenges for the unknown future

Anderberg¹,

Aldskogius²,

Holtz³

2007

Upsala Journal of Medical Sciences

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A neuroprotective role of glial cell line-derived neurotrophic factor following moderate spinal cord contusion injury

Cited by 90 publications

References 63 publications

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

Assessment of White Matter Loss Using Bond-Selective Photoacoustic Imaging in a Rat Model of Contusive Spinal Cord Injury

Spinal cord injury – scientific challenges for the unknown future

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