A multiplex RT-PCR assay for rapid and differential diagnosis of four porcine diarrhea associated viruses in field samples from pig farms in East China from 2010 to 2012

Zhao, Jin; Shi, Baojun; Huang, Xiao-Guo; MingYi, Peng; Zhang, Xiaomin; He, Danni; Pang, Ran; Zhou, Bin; Pu-yan, Chen

doi:10.1016/j.jviromet.2013.08.008

Cited by 41 publications

(42 citation statements)

References 24 publications

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“…While the pathogenesis of PKV is relatively unknown, Park et al suggested that PKV could be the etiological agent of gastroenteritis in pigs based on statistical analysis (Park et al, 2010). Infections with PEDV, PKV, TGEV, and PRoV were all reported in an epidemic of porcine diarrhea in 2010 in China (Cao et al, 2012;Zhao et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

Zp¹,

Yang²,

Wd³

et al. 2016

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Summary. -Piglet diarrhea epidemics result in major economic losses for the swine industry. Four viruses are closely linked to porcine diarrhea: porcine kobuvirus (PKV), porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PRoV). We have conducted an epidemiology study to determine the frequency of infection and co-infection with these viruses in China, and characterized the genetic variation of the isolated PEDV and PKV strains. Stool and intestinal samples (n = 314) were collected from piglets with diarrhea in China from years 2012 to 2014. RT-PCR was used to detect PKV, PEDV, TGEV, and PRoV. Phylogenetic relationships between reference strains and the isolated PEDV and PKV strains were determined based on the M and 3D gene sequence. Th e rates of infection with PKV, PEDV, TGEV and PRoV were 29.9%, 24.2%, 1.91%, and 0.31%, respectively. Co-infections with PKV and the other three viruses were very common. Co-infection of PKV and PEDV was detected in 15.0% (47/314) of the samples. Phylogenetic analysis of the PKV 3D gene indicated that there were some phylogenetic diff erences in the PKV strains across regions within China. However, according to the PEDV M gene, strains clustered into three groups and the primary group was distinct from the vaccine strain CV777. Th is study provides insights in to the prevalence of diarrhea viruses and their prevention and control in China.Keywords: porcine diarrhea viruses; co-infection; porcine kobuvirus; porcine epidemic diarrhea virus; porcine gastroenteritis; porcine rotavirus * Corresponding authors: E-mail: wenjiejin1@163.com, aijian@ yzu.edu.cn; phone: +8613815809571.# Th ese authors contributed equally to this work. Abbreviations: PKV = porcine kobuvirus; PEDV = porcine epidemic diarrhea virus; TGEV = porcine transmissible gastroenteritis virus; ProV = porcine rotavirus

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Section: Introductionmentioning

confidence: 99%

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

Zp¹,

Yang²,

Wd³

et al. 2016

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“…The positive rate of variant PEDV was higher than that of classical PEDV detected by both TaqMan and conventional RT-PCR, indicating that variant PEDV had a high prevalence in the past 3 years. Of course, those PEDV-negative and PEDV-positive samples might contain other diarrhea-causing agents, such as porcine group A rotavirus (GARV), and transmissible gastroenteritis virus (TGEV), which has been reported by others Zhao et al, 2013).…”

Section: Discussionmentioning

confidence: 93%

Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus

Zhao

Bai

Jiang

et al. 2014

Journal of Virological Methods

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“…The clinical symptoms of PED include severe enteritis, vomiting, and watery diarrhea, with high infectivity and lethality in piglets. Since the 2010s, there have been continuous outbreaks of this disease in swine farms in nearly all provinces in China (Chen et al, 2010;Sun et al, 2012;Wang et al, 2013;Zhang et al, 2013;Zhao et al, 2013;Jung and Saif, 2015;Song et al, 2015a). Specifically, during the initial prevalence of the disease in farrowing barns, the mortality of newborn piglets has been found to be approximately 100%, which is responsible for considerable financial losses faced by the swine industry (Shibata et al, 2000;Li et al, 2012;Jung and Saif, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Although most large-scale pig farms were vaccinated according to the proper immune program, immunized swine herds continue to be infected by PEDV (Li et al, 2012;Zhao et al, 2013;Jung and Saif, 2015;Song et al, 2015b) (Li et al, 2012;Zhao et al, 2013;Jung and Saif, 2015;Song et al, 2015b). Therefore, the genetic relationships between different strains must be elucidated to effectively control and prevent PEDV infection.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus samples obtained from farms in Gansu, China

Wang

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Porcine epidemic diarrhea poses significant sanitation problems in the porcine industry, and has negatively affected the economy in recent years. In this study, 48 fecal specimens were collected from piglets from four intensive swine farms located in the Gansu Province of China. The molecular diversity and phylogenetic relationships between porcine epidemic diarrhea viruses (PEDV) prevalent in Gansu were probed, and the resultant proteins were characterized. Sequence analysis of the spike protein (S) genes showed that each specimen had unique characteristics, and that the PEDV1/S/4 strain could be differentiated from the others via a unique mutation of the S gene. The phylogeny of S glycoprotein showed that all strains were clustered into two major groups. The four Gansu PEDV field strains were characterized into different groups; this finding was consistent with the results of the protein characterization prediction. This analysis additionally revealed the unique characteristics of each specimen. The results of this study could be used to elucidate the prevalence of PEDV and contribute to the prevention of PEDV in Gansu.

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A multiplex RT-PCR assay for rapid and differential diagnosis of four porcine diarrhea associated viruses in field samples from pig farms in East China from 2010 to 2012

Cited by 41 publications

References 24 publications

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

The rate of co-infection for piglet diarrhea viruses in China and the genetic characterization of porcine epidemic diarrhea virus and porcine kobuvirus

Development of a multiplex TaqMan probe-based real-time PCR for discrimination of variant and classical porcine epidemic diarrhea virus

Molecular characterization and phylogenetic analysis of porcine epidemic diarrhea virus samples obtained from farms in Gansu, China

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