A multi-site clinical trial evaluating PICSI® versus intracytoplasmic sperm injection (ICSI): positive clinical outcomes observed in a prospective, randomized and double-blinded study

Worrilow, K.C.; Eid, Sherrine; Matthews, J.M.; Pelts, E.J.; Khoury, C.; Liebermann, J.

doi:10.1016/j.fertnstert.2009.07.141

Cited by 8 publications

(3 citation statements)

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“…The HspA 2 also acts as a hyaluronic acid (HA) receptor during normal fertilisation (Huszar et al ., ; Parmegiani et al ., ). It has been shown that HA‐bound spermatozoa have increased developmental maturity, increased chromatin integrity, increased morphology, increased functional competence (Keel et al ., ), decreased aneuploidy and decreased active caspase‐3 (Cayli et al ., ; Jakab et al ., ; Worrilow et al ., ). This protein localised in several organs, including the spermatozoa's surface (Ghosh & Datta, ; Ghosh et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…In a multicentre clinical trial of Worrilow et al . (), it was demonstrated that when patients with <65% binding efficiency are pre‐screened and HA‐bound spermatozoa is selected for ICSI, their success rates and implantation rates were slightly higher. However, a significant reduction in pregnancy loss was seen by them for the HA group, 3.3% versus 15.1% for the conventional ICSI group ( P = 0.021).…”

Section: Discussionmentioning

confidence: 99%

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Relationship between human spermatozoa-hyaluronan-binding assay, conventional semen parameters and fertilisation rates in intracytoplasmic spermatozoa injection

Esterhuizen¹,

Franken

Bosman³

et al. 2014

Andrologia

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Selecting the best spermatozoa for intracytoplasmic spermatozoa injection (ICSI) has recently been a topic of great interest among embryologists. The study aimed to evaluate the relationship between the spermatozoa-hyaluronan-binding assay (HBA), routine semen analysis results and fertilisation rates as recorded during conventional ICSI therapy. Ninety-one patients undergoing conventional ICSI treatment in the Medfem Fertility Clinic in Johannesburg (South Africa) were included in the study. A total of 797 oocytes were injected of which 457 oocytes fertilised (57.3%, range 0-100%). None of the semen parameters correlated with the fertilisation rates (Table 2). HBA scores, however, revealed a highly significant association (p ≤ 0.0001) with the fertilisation rates. The HBA scores also correlated significantly with the biochemical pregnancy values (Spearman r = 0.24, P = 0.02, 95% CI 0.039-0.43); however, the HBA scores did not correlate with the clinical pregnancy rates (Spearman r = 0.14, P = 0.16, 95% CI -0.06 to 0.34). No correlation was recorded between HBA and the standard semen parameters. The study showed that HBA is significantly associated with fertilisation in conventional ICSI. The HBA scores were also significantly associated with the fertilisation rates and biochemical pregnancies.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Relationship between human spermatozoa-hyaluronan-binding assay, conventional semen parameters and fertilisation rates in intracytoplasmic spermatozoa injection

Esterhuizen¹,

Franken

Bosman³

et al. 2014

Andrologia

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“…The head of motile mature spermatozoa presenting sufficient numbers of PH20 or HA receptors firmly binds to the solid state HA without showing forward motion of the head whereas freely moving spermatozoa are immature or defective with insufficient HA receptors. Literature studies on the PICSI procedure are mainly related to oral and poster presentations, which suggest that the PICSI procedure improves fertilization, implantation and pregnancy rates (Sanchez et al, 2005;Janssens et al, 2006;Worrilow et al, 2009). In these studies, clinical pregnancy was significantly improved when the PICSI procedure was implemented in the cases with low HA-binding ability.…”

Section: Selection Of Spermatozoa Based On Their Ability To Bind Hyalmentioning

confidence: 99%

New era in sperm selection for ICSI

2011

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Spermatozoa contribute to approximately half of the genome of future progeny, and therefore, have a profound impact on embryo development post-fertilization. Sperm selection based on viability and normal morphology does not eliminate the chance for DNA damaged spermatozoa to be inseminated and may account for a considerable percentage of failed embryo development post-ICSI (Intra cytoplasmic sperm injection). Therefore, sperm selection based on functional sperm characteristics to preclude insemination of DNA damaged spermatozoa have paved the way for successful ICSI outcomes. In regard to this, different laboratories have introduced novel procedures to replace traditional or orthodox sperm selection methods. This review attempts to provide information on the scientific bases of each procedure, and pinpoint their advantages and disadvantages. In addition to data from our research, a systematic search on the literature, publications and presentations was carried out using such databases as PubMed and ISI-Web.

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