A modified QuEChERS method as sample treatment before the determination of isoflavones in foods by ultra-performance liquid chromatography–triple quadrupole mass spectrometry

Delgado-Zamarreño, M.M.; Pérez‐Martín, Lara; Bustamante-Rangel, Myriam; Carabias‐Martínez, Rita

doi:10.1016/j.talanta.2012.07.070

Cited by 36 publications

(22 citation statements)

References 41 publications

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“…However, there are also examples of adoptions of this approach for extraction of compounds such as isoflavones and carotenoids (fucoxanthin), which are naturally occurring substances deemed as beneficial to health. Delgado-Zamarreño et al developed an analytical method for the determination of isoflavones in legumes using LC-MS/MS [98]. A modified QuEChERS was used by authors to extract the analytes from the food samples such as chickpeas, lentils, and beans.…”

Section: Natural Compounds Analysismentioning

confidence: 99%

“…Under these conditions, the clean-up step was not necessary. The method proposed by DelgadoZamarreño et al was precise, selective and not timeconsuming, with recoveries ranging from 72 to 119% and standard deviations lower than 25% for the interday precision [98]. Piovan et al applied the QuEChERS method to obtain fucoxanthin extracts from Undaria pinnatifida, a seaweed rich in this carotenoid [99].…”

Section: Natural Compounds Analysismentioning

confidence: 99%

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A review of recent developments and trends in the QuEChERS sample preparation approach

2015

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Abstract:A comprehensive review is presented on the recent developments and trends in the QuEChERS (quick, easy, cheap, effective, rugged, and safe) sample preparation approach. This technique involves liquidliquid partitioning using acetonitrile and purifying the extract using dispersive solid-phase extraction (d-SPE). Originally, the QuEChERS was introduced for pesticides residues analysis in high moisture fruits and vegetables, but more recently it is gaining significant popularity in the analysis of broad spectrum of analytes in huge variety of samples. The wide range of the technique applications is possible due to introducing various modifications based on the use of different extraction solvent and salt formulation and buffer additions for salting-out partitioning step and the application of various d-SPE sorbents for clean-up step. Therefore, the QuEChERS approach is useful for analysis of, among others pesticides, veterinary drugs and other pharmaceuticals, mycotoxins, polycyclic aromatic hydrocarbons (PAHs), dyes, acrylamide, synthetic musks and UV filters, bisphenols, polybrominated diphenyl ethers and other flame retardants, endocrine disruptors, and other chemical compounds. Thanks to the QuEChERS approach, high-throughput multiresidue methods operate in a routine contaminant control of food products, feedstuff, and environmental samples.

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Section: Natural Compounds Analysismentioning

confidence: 99%

Section: Natural Compounds Analysismentioning

confidence: 99%

A review of recent developments and trends in the QuEChERS sample preparation approach

2015

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“…In a previous work chromatographic separation and MS/MS detection were optimised (Delgado‐Zamarreño et al ., ). The mobile phase consisted of an acetonitrile (solvent A) and 0.01% aqueous formic acid (solvent B) gradient at a flow rate of 0.5 mL/min.…”

Section: Methodsmentioning

confidence: 99%

“…In previous work we extracted isoflavones from both liquid (Bustamante‐Rangel et al ., ) and solid (Delgado‐Zamarreño et al ., ) samples using solvent extraction, either alone or with the addition of salts, using a modified QuEChERS (Quick Easy Cheap Effective Rugged Safe) methodology. This latter approach was applied to the extraction of eight isoflavones, including aglycones and glucosides, from legume samples.…”

Section: Introductionmentioning

confidence: 97%

Comparative Study of the Methodology Used in the Extraction of Isoflavones from Legumes Applying a Modified QuEChERS Approach

Bustamante‐Rangel

Pérez‐Martín

Delgado-Zamarreño

2013

Phytochemical Analysis

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“…By binding to estrogen receptors, they mimic or modulate the effect on the endogenous receptors. Studies have shown that daidzein is the isoflavone with the highest biological activity, followed by genistein . They are present not only in high concentrations in soy, clover, beans and peas but also in other legumes such as lentils and chickpeas.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a high-resolution LTQ Orbitrap MS method for the quantification of isoflavones in wastewater effluent

et al. 2015

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Isoflavones and coumestranes are the most important classes of compounds among phytoestrogens; by binding to estrogen receptors, they mimic or modulate the effect on the endogenous receptors. Little information can be found in literature about the presence of isoflavones and coumestrol in the environment, even if it is known that this may have significance, being these substances classified as endocrine disrupting compounds. In this research, we aim to explore the capabilities of the LTQ Orbitrap Discovery hybrid MS in full-scan acquisition mode, with high resolution, to validate an analytical method for the quantification of nine isoflavones (genistein, genistin, glycitein, daidzein, daidzin, (R,S)-equol, biochanin A, formononetin and coumestrol) in wastewater samples. The correlation coefficients of calibration curves of the nine analyzed compounds were in a range of 0.996-0.999; recoveries at two different levels of concentration (0.05 and 0.5 µg/l) were in the range 73-98%, and the limits of detection ranged between 0.0014 and 0.017 µg/l, proving that this method is sensitive enough in comparison with other methods available in literature. This method has been applied for the analysis of 20 wastewater treatment plants in County Cork, Ireland.

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A modified QuEChERS method as sample treatment before the determination of isoflavones in foods by ultra-performance liquid chromatography–triple quadrupole mass spectrometry

Cited by 36 publications

References 41 publications

A review of recent developments and trends in the QuEChERS sample preparation approach

A review of recent developments and trends in the QuEChERS sample preparation approach

Comparative Study of the Methodology Used in the Extraction of Isoflavones from Legumes Applying a Modified QuEChERS Approach

Development and validation of a high-resolution LTQ Orbitrap MS method for the quantification of isoflavones in wastewater effluent

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