A model for apoptotic interaction between white spot syndrome virus and shrimp

Leu, Jiann Horng; Lin, San‐Yih; Huang, Jiun Yan; Chen, Tsan‐Chi; Lo, Chu Fang

doi:10.1016/j.fsi.2012.05.030

Cited by 77 publications

(66 citation statements)

References 63 publications

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“…Both apoptosis and shrimp AMPs, including Penaeidins (PENs), crustins, and anti-lipopolysaccharide factors (ALFs), are important in the defense against WSSV infection [25–35]. Apoptosis induced by WSSV infection is important for successful WSSV pathogenesis [25–27,31]. To manipulate host apoptosis, WSSV modulates the expression of shrimp apoptosis-related genes, such as PmCasp , PjCaspase , Pm-fortilin and VDAC , to actively promote apoptosis to spread virus progeny to neighboring cells; in contrast, WSSV also encodes two anti-apoptosis proteins, AAP-1 (ORF390 or WSSV449) and WSSV222, to block apoptosis in order to prevent premature host cell death and maximize virus progeny [27–29,36–40].…”

Section: Introductionmentioning

confidence: 99%

“…Apoptosis induced by WSSV infection is important for successful WSSV pathogenesis [25–27,31]. To manipulate host apoptosis, WSSV modulates the expression of shrimp apoptosis-related genes, such as PmCasp , PjCaspase , Pm-fortilin and VDAC , to actively promote apoptosis to spread virus progeny to neighboring cells; in contrast, WSSV also encodes two anti-apoptosis proteins, AAP-1 (ORF390 or WSSV449) and WSSV222, to block apoptosis in order to prevent premature host cell death and maximize virus progeny [27–29,36–40]. The function of one shrimp IAP in regulating hemocyte apoptosis has been studied [41,42]; however, the roles of shrimp IAPs in defending against WSSV infection and regulating AMP expression through the NF-κB pathway remain unknown [41,42].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Expression, Cellular Localization, and Function of Three Inhibitors of Apoptosis (IAPs) from Litopenaeus vannamei during WSSV Infection and in Regulation of Antimicrobial Peptide Genes (AMPs)

Wang

Wan

et al. 2013

PLoS ONE

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Inhibitors of apoptosis (IAPs) play important roles in apoptosis and NF-κB activation. In this study, we cloned and characterized three IAPs (LvIAP1-3) from the Pacific white shrimp, Litopenaeusvannamei . LvIAP1-3 proteins shared signature domains and exhibited significant similarities with other IAP family proteins. The tissue distributions of LvIAP1-3 were studied. The expression of LvIAP1-3 was induced in the muscle after white spot syndrome virus (WSSV) infection. LvIAP1 expression in the gill, hemocytes, hepatopancreas, and intestine was responsive to WSSV and Vibrio alginolyticus infections. LvIAP2 expression in the gill, hemocytes, and hepatopancreas was also responsive to WSSV infection. The expression of LvIAP3 in the gill, hemocytes, and intestine was reduced after V . alginolyticus infection. When overexpressed in Drosophila S2 cells, GFP labeled-LvIAP2 was distributed in the cytoplasm and appeared as speck-like aggregates in the nucleus. Both LvIAP1 and LvIAP3 were widely distributed throughout the cytoplasm and nucleus. The expression of LvIAP1, LvIAP2, and LvIAP3 was significantly knocked down by dsRNA-mediated gene silencing. In the gill of LvIAP1- or LvIAP3-silenced shrimp, the expression of WSSV VP28 was significantly higher than that of the dsGFP control group, suggesting that LvIAP1 and LvIAP3 may play protective roles in host defense against WSSV infection. Intriguingly, the LvIAP2-silenced shrimp all died within 48 hours after dsLvIAP2 injection. In the hemocytes of LvIAP2-silenced shrimps, the expression of antimicrobial peptide genes (AMPs), including Penaeidins, lysozyme, crustins, Vibrio penaeicidae-induced cysteine and proline-rich peptides (VICPs), was significantly downregulated, while the expression of anti-lipopolysaccharide factors (ALFs) was upregulated. Moreover, LvIAP2 activated the promoters of the NF-κB pathway-controlled AMPs, such as shrimp Penaeidins and Drosophila drosomycin and attacin A, in Drosophila S2 cells. Taken together, these results reveal that LvIAP1 and LvIAP3 might participate in the host defense against WSSV infection, and LvIAP2 might be involved in the regulation of shrimp AMPs.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of Expression, Cellular Localization, and Function of Three Inhibitors of Apoptosis (IAPs) from Litopenaeus vannamei during WSSV Infection and in Regulation of Antimicrobial Peptide Genes (AMPs)

Wang

Wan

et al. 2013

PLoS ONE

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“…Hepatopancrea was an important organ of shrimp metabolize. Some research had focused on genes, biochemical and physiological changes related to WSSV infection in shrimpand differentially expressed proteins in the hepatopancrea of shrimp (Yoganandhan 2003;Leu et al 2007Leu et al , 2013Li et al 2013) and upregulation and down-regulation proteins were identified. The above studies show that the host's metabolism will be affected after the WSSV virus invades, and this negative effect will lead to the shrimp's immune capacity decline or lead to death.…”

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confidence: 99%

A metabolic study in hepatopancreas of Litopenaeus vannamei response to white spot syndrome virus

Zhang

et al. 2017

Int Aquat Res

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White spot syndrome virus (WSSV) cause great harm in shrimp aquaculture. To understand the impact of viral infection on the shrimp metabolism, we monitored the culture farms of Litopenaeus vannamei and collected the samples on different stages of WSSV infection. The hepatopancreas of shrimp were separated, and then used gas chromatography mass spectrometry to detect the metabolites. Through the mass spectrometric analysis combined with multivariate data analysis, including PCA and OPLS models, metabolism of the shrimp was significantly changed by WSSV infection. The data indicated that in the early stage of WSSV infection, the glycolysis changed significantly, the contents of glucose and lactate increased distinctly. The metabolites of TCA cycle did not show up obviously regularity. The organism of fatty acids showed the same situation with glycolysis. At the early stage of infection, 14 amino acids metabolism were up-regulated, and glycine still increased at later stage of infection and the concentration was increased twice. The data of this study may provide some information to further research of viral disease mechanism.

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Section: Discussionmentioning

confidence: 99%

“…WSSV infection modulates the expression of several shrimp apoptosis-related genes against viral infection (Leu et al, 2013). Caspase, one of main affector of apoptotic process, has been shown to participate in shrimp antiviral immunity (Leu et al, 2013). Down-regulation of this gene by siRNA silencing before WSSV challenge resulted in a decrease in the number of apoptotic hemocytes and an increase in the number of WSSV copies in the hemolymph in M. japonicas (L. .…”

Section: Discussionmentioning

confidence: 99%

Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA

et al. 2015

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A model for apoptotic interaction between white spot syndrome virus and shrimp

Cited by 77 publications

References 63 publications

Analysis of Expression, Cellular Localization, and Function of Three Inhibitors of Apoptosis (IAPs) from Litopenaeus vannamei during WSSV Infection and in Regulation of Antimicrobial Peptide Genes (AMPs)

Analysis of Expression, Cellular Localization, and Function of Three Inhibitors of Apoptosis (IAPs) from Litopenaeus vannamei during WSSV Infection and in Regulation of Antimicrobial Peptide Genes (AMPs)

A metabolic study in hepatopancreas of Litopenaeus vannamei response to white spot syndrome virus

Enhancement of shrimp immunity against white spot syndrome virus by Macrobrachium rosenbergii nodavirus-like particle encapsulated VP28 double-stranded RNA

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