A microsatellite marker based linkage map of tobacco

Abstract

We report the first linkage map of tobacco (Nicotiana tabacum L.) generated through microsatellite markers. The microsatellite markers were predominantly derived from genomic sequences of the Tobacco Genome Initiative (TGI) through bioinformatics screening for microsatellite motives. A total of 684 primer pairs were screened for functionality in a panel of 16 tobacco lines. Of those, 637 primer pairs were functional. Potential parents for mapping populations were evaluated for their polymorphism level through genetic similarity analysis. The similarity analysis revealed that the known groups of tobacco varieties (Burley, Flue-cured, Oriental and Dark) form distinct clusters. A mapping population, based on a cross between varieties Hicks Broad Leaf and Red Russian, and consisting of 186 F2 individuals, was selected for mapping. A total of 282 functional microsatellite markers were polymorphic in this population and 293 loci could be mapped together with the morphological trait flower color. Twenty-four tentative linkage groups spanning 1,920 cM could be identified. This map will provide the basis for the genetic mapping of traits in tobacco and for further analyses of the tobacco genome.

Mixed linear model association mapping for low chloride accumulation rate in oriental-type tobacco (Nicotiana tabaccum L.) germplasm

“…The D′ values ranged from 0.01 to 0.82 with the average value of 0.268. Using MLM procedure based on Q + K model 1 SSR locus (pt30027) from linkage group 13 (Bindler et al 2007) was identified that possessed significant association (P ≤ 0.04) with the QTL controlling chloride accumulation rates in oriental-type tobacco genotypes (Table 2).…”

“…Twenty-six SSR primer pairs out of 278 from the tobacco SSR database (Bindler et al 2007(Bindler et al , 2011 were used for DNA fingerprinting. The choice of SSR markers was based on their known genetic locations to obtain near-uniform coverage of the tobacco genome and clarity of produced bands (Bindler et al 2007(Bindler et al , 2011. PCR was performed in a 20 µl volume using a 96-well Eppendorf Mastercycler Gradient (Type 5331, Eppendorf AG, Hamburg, Germany).…”

High-throughput Method for Determination of Seed Paternity by Microsatellite Markers

“…However, this does not include a protocol for the novel marker selection/identification, but rather exploits the markers generated for a closely related species N.tabacum (Bindler et al, 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data.…”

“…tabacum (Bindler et al, 2007). This is a high-throughput protocol optimized and streamlined for one skilled person to process 384 (96 x 4) seeds in 5 days, from DNA isolation (from seedlings) to paternity assessment by microsatellite genotype data.…”

Desenvolvimento de marcador molecular para resistência a Tobacco mosaic virus e herança da resistência a Meloidogyne incognita raça 3 em tabaco

“…Nicotiana pertence à família das solanáceas e subdivide-se em três subgenêros: Rustica, Tabaccum e Petunioides (GOODSPEED, 1954 apud REN;TIMKO, 2001). O gênero Nicotiana, além da relevância econômica, é considerado um importante modelo para estudo na área de biotecnologia e, ainda, como auxiliar em estudos que envolvem princípios de resistência a doenças, síntese de metabólitos secundários e fisiologia de plantas (BINDLER et al, 2007).…”