A microperfusion investigation of sodium resorption and potassium secretion by the main excretory duct of the rat submaxillary gland

Ja, Young; Frömter, Eberhard; Schögel, E; Hamann, Karl-Friedrich

doi:10.1007/bf00362747

Cited by 100 publications

(26 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is also interesting to note that there is a striking similarity in the rate of K+ flux between the two epithelia. In the perfused submaxillary duct 48 K-SECRETION BY RAT ECCRINE SWEAT GLAND of 13 mm2 surface area (Young et al 1967), isoproterenol and carbachol-induced increase in net K+ flux (into the lumen) of 4-4 and 9 0 n-equiv./min. duct, respectively, near the steady-state condition, yield the K+ flux of 0 34 and 0*7 n-equiv/ min.…”

Section: Discussionmentioning

confidence: 99%

Secretion of a potassium‐rich fluid by the secretory coil of the rat paw eccrine sweat gland

Sato

1978

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Secretion of a potassium‐rich fluid by the secretory coil of the rat paw eccrine sweat gland

Sato

1978

The Journal of Physiology

View full text Add to dashboard Cite

show abstract

“…This implies not only that the excurrent ducts secrete potassium but also that they increase their secretoiy rate part passu with increasing rate of formation of primary fluid so that final salivary potassium concentration remains constant; this phenomenon has been termed acino-tubniar balance (Martin and Young, 1971a). Ductal secretion of potassium in the rat submaxillary gland was demonstrated directly by microperfrision (Young et al, 1967) and, when snbsequently parasympathomimetic drugs were .shown to stimulate dnctal potas.sium .secretion (Young et al, 1970), it was postulated that acino-tubular balance could lie due to this stimulation. Potassium concentrations in human saliva (Thaysen, Thorn and Schwartz, 1954;Knanf and Fromter, 1970) aud in the saliva of otlier primates (Brnckman and Mangos, 1970;Siegel, 1972) are high and ductal secretion of potassium can be presuined to behave similarly.…”

Section: Formation Of Final Mliva-fhe Role Of the Excurrant Ductsmentioning

confidence: 99%

Water and Electrolyte Excretion in the Cat Submaxillary Gland Studied Using Micropuncture and Duct Cannulation Techniques

Kaladelfos

1974

Aust J Exp Biol Med

View full text Add to dashboard Cite

Summary. Studies on the electrolyte compositioii of the adnar (or primary) secretion and the final saliva of the cat submaxillary gland untlerfjoing parasympatho-and sympathomimetic stimulation have been undertaken using uiicropuncture and duct cannulation techniques for sample collection. The work was undertaken to see to what extent the two-stage secretory model developed previously for rat salivary glands would hold for rather different glands of other species. We find that in genera! tenns the .same two-stage model is apphcable. The primary secretion in both species has plasma-like .sodium and chloride concentrations and potassium concentrations about twice those of plasma; gland stimulation does not greatly alter the composition of this fluid. In the secondary stage, during passage of this fluid altnig the gland excurrent ducts, there is. in both species, extensive ductai absorption of sodium and chloride, although for the cat the absorptive mechanism is more easily saturable. Unlike rat submaxillary ducts, however, those of the cat appear to secrete little potassium. and gland stimulation does not increase the secretory rate of this ion. so that the phenomenon termed acino-tubular balance is not observed. Although both ducts may secrete bicarbonate this is much more conspicuous for the rat. In contrast to parasympathomimetic stimulation, the effects of sympathomimetic stimulation on the two glands were totally different, In the cat a much more vigorous secretorj' response was obtained than in the rat but the striking elevation of salivar>' potassium and bicarbonate concentrations to values in excess of 100 niM seen in the rat did not occur. It Ls concluded that while a two-stage hypothesis may account for secretion of water and electrolytes in many salivary glands, the role of ductai stimulation in the secondary stage and the relative roles of sympathetic and parasympathetic stimulation may vary greatly among species. INTRODUGTION.The adaptation of renal micropuncture techniques to the study of electrolyte transport in .salivan' glands (Martinez, Holzgreve and Frick, 1966;Young and Schogel, 1966) has tlu^own a great deal of light on their secretory mechanisms. For reasons of convenience these micropuncture experiments were carried out 68on the salivary glands of only one species, the rat, although it was soon realized that most rat exocrlne secretions are rather atypical in ha\'ing extremely high potas.sium concentrations. This holds not only for the three major salivary glands, the submaxillary (Young and Schogel, 1966), the sublingual (Martin and Young, 1971a) and the parotid (Mangos and McSherry, 1969), but also for rat sweat glands (Brusilow, Ikai and Gordes, 196S) and lacrinial glands (Alexander, van Lennep and Young, 1972) as well as for the pancreas (Sewell and Young, unpublished observations). It seemed highly desirable to extend our .studies to include a hypotonicsecreting salivary gland from another species in which salivary potassium concentrations were more typical of exocrine glands in ge...

show abstract

“…The primary saliva is an isotonic plasma-like fluid, which is secreted from acinar cells and, in most species, is subsequently modified in the duct system by secretion of K ϩ and HCO 3 Ϫ and absorption of Na ϩ and Cl Ϫ (Young et al 1967(Young et al ,1970Young 1968;Knauf et al 1982;Paulais et al 1994). Current models of HCO 3 Ϫ secretion in the main excretory duct of the rat submandibular gland propose the presence of luminally located Na ϩ /H ϩ -, K ϩ /H ϩ -, and Cl Ϫ /HCO 3 Ϫ exchangers together with Na ϩ and Cl Ϫ channels and a basolaterally located Na ϩ pump, a K ϩ channel, and an additional Na ϩ /H ϩ exchanger (Young et al 1967(Young et al , 1970Young 1968;Schneyer 1968Schneyer ,1969.…”

mentioning

confidence: 99%

“…Studies of secretory mechanisms in the duct system were originally performed in the main excretory duct because of its accessibility for microperfusion studies (Young 1968;Schneyer 1968Schneyer ,1969Young et al 1967Young et al , 1970Knauf et al 1982). However, the main excretory duct represents only a small portion of the salivary duct tissue, the majority being made up of intralobular ducts.…”

mentioning

confidence: 99%

Immunolocalization of Vacuolar-type H⁺-ATPase in Rat Submandibular Gland and Adaptive Changes Induced by Acid–Base Disturbances

Roussa

Thévenod

Sabolić

et al. 1998

J Histochem Cytochem.

View full text Add to dashboard Cite

SUMMARYUsing antibodies against the 31-kD and 70-kD subunits of vacuolar type H ϩ -ATPase (V-ATPase) and light microscopic immunocytochemistry, we have demonstrated the presence of this V-ATPase in rat submandibular gland. We have also investigated the adaptive changes of this transporter during acid-base disturbances such as acute and chronic metabolic acidosis or alkalosis. Our results show intracellularly distributed V-ATPase in striated, granular, and main excretory duct cells in controls, but no V-ATPase immunoreaction in acinar cells. Both acute and chronic metabolic acidosis caused a shift in V-ATPase away from diffuse distribution towards apical localization in striated and granular duct cells, suggesting that a V-ATPase could be involved in the regulation of acid-base homeostasis. In contrast, during acidosis the main excretory duct cells showed no changes in the V-ATPase distribution compared to controls. With acute and chronic metabolic alkalosis, no changes in the V-ATPase distribution occurred. (J Histochem Cytochem 46:91-100, 1998)

show abstract

A microperfusion investigation of sodium resorption and potassium secretion by the main excretory duct of the rat submaxillary gland

Cited by 100 publications

References 16 publications

Secretion of a potassium‐rich fluid by the secretory coil of the rat paw eccrine sweat gland

Secretion of a potassium‐rich fluid by the secretory coil of the rat paw eccrine sweat gland

Water and Electrolyte Excretion in the Cat Submaxillary Gland Studied Using Micropuncture and Duct Cannulation Techniques

Immunolocalization of Vacuolar-type H⁺-ATPase in Rat Submandibular Gland and Adaptive Changes Induced by Acid–Base Disturbances

Contact Info

Product

Resources

About

A microperfusion investigation of sodium resorption and potassium secretion by the main excretory duct of the rat submaxillary gland

Cited by 100 publications

References 16 publications

Secretion of a potassium‐rich fluid by the secretory coil of the rat paw eccrine sweat gland

Secretion of a potassium‐rich fluid by the secretory coil of the rat paw eccrine sweat gland

Water and Electrolyte Excretion in the Cat Submaxillary Gland Studied Using Micropuncture and Duct Cannulation Techniques

Immunolocalization of Vacuolar-type H+-ATPase in Rat Submandibular Gland and Adaptive Changes Induced by Acid–Base Disturbances

Contact Info

Product

Resources

About

Immunolocalization of Vacuolar-type H⁺-ATPase in Rat Submandibular Gland and Adaptive Changes Induced by Acid–Base Disturbances